产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12442, RRID:AB_2845247.
展开/折叠
EC 3.1.1.47; HSD PLA2; HSDPLA2; Platelet activating factor acetylhydrolase 2; Platelet activating factor acetylhydrolase 2 cytoplasmic; platelet-activating factor acetylhydrolase 2, 40kDa; SD PLA2; Serine dependent phospholipase A2;
抗原和靶标
Broadly expressed in different tissues, but high in B- and T-lymphocytes. In brain, expression is restricted to amygdala and frontal cortex.
- Q99487 PAFA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGVNQSVGFPPVTGPHLVGCGDVMEGQNLQGSFFRLFYPCQKAEETMEQPLWIPRYEYCTGLAEYLQFNKRCGGLLFNLAVGSCRLPVSWNGPFKTKDSGYPLIIFSHGLGAFRTLYSAFCMELASRGFVVAVPEHRDRSAATTYFCKQAPEENQPTNESLQEEWIPFRRVEEGEKEFHVRNPQVHQRVSECLRVLKILQEVTAGQTVFNILPGGLDLMTLKGNIDMSRVAVMGHSFGGATAILALAKETQFRCAVALDAWMFPLERDFYPKARGPVFFINTEKFQTMESVNLMKKICAQHEQSRIITVLGSVHRSQTDFAFVTGNLIGKFFSTETRGSLDPYEGQEVMVRAMLAFLQKHLDLKEDYNQWNNLIEGIGPSLTPGAPHHLSSL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99487 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y101 | Phosphorylation | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| K176 | Acetylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| K364 | Ubiquitination | Uniprot |
研究背景
Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme.
Cytoplasm. Membrane>Lipid-anchor. Endoplasmic reticulum membrane>Lipid-anchor.
Note: In resting cells, localizes to intracellular membranes and cytoplasm. Translocates from the cytoplasm to intracellular membranes upon oxidative stress.
Broadly expressed in different tissues, but high in B- and T-lymphocytes. In brain, expression is restricted to amygdala and frontal cortex.
Monomer.
Belongs to the serine esterase family.
研究领域
· Metabolism > Lipid metabolism > Ether lipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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