| 产品: | PDHX 抗体 |
| 货号: | DF12448 |
| 描述: | Rabbit polyclonal antibody to PDHX |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat, Monkey |
| 预测: | Pig, Bovine, Horse, Sheep, Dog |
| 分子量: | 54 kDa; 54kD(Calculated). |
| 蛋白号: | O00330 |
| RRID: | AB_2845253 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12448, RRID:AB_2845253.
展开/折叠
Dihydrolipoamide dehydrogenase binding protein of pyruvate dehydrogenase complex; Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; DLDBP; E3 binding protein; E3-binding protein; E3BP; Lipoyl containing pyruvate dehydrogenase complex component X; Lipoyl-containing pyruvate dehydrogenase complex component X; mitochondrial; ODPX_HUMAN; OPDX; PDHX; PDX 1; PDX1; Pro X; proX; Pyruvate dehydrogenase complex component X; Pyruvate dehydrogenase complex lipoyl containing component X; Pyruvate dehydrogenase complex, E3 binding protein subunit; Pyruvate dehydrogenase protein X component; Pyruvate dehydrogenase protein X component mitochondrial;
抗原和靶标
- O00330 ODPX_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASWRLGCDPRLLRYLVGFPGRRSVGLVKGALGWSVSRGANWRWFHSTQWLRGDPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVEEGSKNIRLGSLIGLIVEEGEDWKHVEIPKDVGPPPPVSKPSEPRPSPEPQISIPVKKEHIPGTLRFRLSPAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTPAPTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEIPASNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRQDLVKDDIKVSVNDFIIKAAAVTLKQMPDVNVSWDGEGPKQLPFIDISVAVATDKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTEDEEGNAKLQQRQLITVTMSSDSRVVDDELATRFLKSFKANLENPIRLA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O00330 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y16 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| K120 | Acetylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| T179 | Phosphorylation | Uniprot | |
| K194 | Acetylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| R208 | Methylation | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| T369 | Phosphorylation | Uniprot | |
| T375 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K460 | Ubiquitination | Uniprot | |
| K488 | Acetylation | Uniprot | |
| K491 | Acetylation | Uniprot | |
| K491 | Methylation | Uniprot |
研究背景
Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.
Delipoylated at Lys-97 by SIRT4, delipoylation decreases the PHD complex activity.
Mitochondrion matrix.
Part of the inner core of the multimeric pyruvate dehydrogenase complex that is composed of about 48 DLAT and 12 PDHX molecules. This core binds multiple copies of pyruvate dehydrogenase (subunits PDH1A and PDHB, E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). Interacts with SIRT4. Interacts with DLD.
Belongs to the 2-oxoacid dehydrogenase family.
研究领域
· Metabolism > Global and overview maps > Metabolic pathways.
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