Seladin 1 Antibody - #DF12472

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产品: Seladin 1 抗体
货号: DF12472
描述: Rabbit polyclonal antibody to Seladin 1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 60 kDa; 60kD(Calculated).
蛋白号: Q15392
RRID: AB_2845277

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(90%)
克隆:
Polyclonal
特异性:
Seladin 1 Antibody detects endogenous levels of total Seladin 1.
RRID:
AB_2845277
引用格式: Affinity Biosciences Cat# DF12472, RRID:AB_2845277.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

24 dehydrocholesterol reductase; 24-dehydrocholesterol reductase; 3 beta hydroxysterol delta 24 reductase; 3-beta-hydroxysterol delta-24-reductase; DCE; Delta(24)-sterol reductase; Desmosterol to cholesterol enzyme; DHC24_HUMAN; Dhcr24; Diminuto dwarf1 homolog; Diminuto/dwarf1 homolog; KIAA0018; Nbla03646; Seladin-1; SELADIN1; Selective AD indicator 1; Selective Alzheimer's Disease Indicator 1;

抗原和靶标

免疫原:
Uniprot:

Q15392(DHC24_HUMAN) >>Visit HPA database.

基因/基因ID:
DHCR24(1718)
表达:
Q15392 DHC24_HUMAN:

Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. In the brain, strongly expressed in cortical regions, substantia nigra, caudate nucleus, hippocampus, medulla oblongata and pons. In brains affected by Alzheimer disease, expression in the inferior temporal lobe is substantially lower than in the frontal cortex.

序列:
MEPAVSLAVCALLFLLWVRLKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKTHKNIMINLMDILEVDTKKQIVRVEPLVTMGQVTALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRCTPSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGLEAICAKFTHESQRQENHFVEGLLYSLDEAVIMTGVMTDEAEPSKLNSIGNYYKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCPFILPSQPGLVHPKGNEAELYIDIGAYGEPRVKHFEARSCMRQLEKFVRSVHGFQMLYADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAARH

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
90
Zebrafish
80
Chicken
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15392 作为底物

Site PTM Type Enzyme
K76 Ubiquitination
K82 Ubiquitination
K87 Ubiquitination
T110 Phosphorylation
Y180 Phosphorylation
T188 Phosphorylation
Y190 Phosphorylation
S198 Phosphorylation
K254 Ubiquitination
Y299 Phosphorylation
Y300 Phosphorylation
K301 Ubiquitination
K313 Methylation
K313 Ubiquitination
Y321 Phosphorylation
K367 Ubiquitination
T373 Phosphorylation
K376 Ubiquitination
K427 Ubiquitination
K446 Sumoylation
K446 Ubiquitination
K459 Ubiquitination
K492 Ubiquitination
K496 Ubiquitination
Y507 Phosphorylation
K509 Ubiquitination
K512 Ubiquitination

研究背景

功能:

Catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. In addition to its cholesterol-synthesizing activity, can protects cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis.

细胞定位:

Endoplasmic reticulum membrane>Single-pass membrane protein. Golgi apparatus membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. In the brain, strongly expressed in cortical regions, substantia nigra, caudate nucleus, hippocampus, medulla oblongata and pons. In brains affected by Alzheimer disease, expression in the inferior temporal lobe is substantially lower than in the frontal cortex.

亚基结构:

Interacts with DHCR7; this interaction regulates DHCR7 activity.

蛋白家族:

Belongs to the FAD-binding oxidoreductase/transferase type 4 family.

研究领域

· Metabolism > Lipid metabolism > Steroid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Effects of Fdft 1 gene silencing and VD3 intervention on lung injury in hypoxia-stressed rats. Genes & Genomics, 2022 (PubMed: 35947298) [IF=2.1]

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