产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12474, RRID:AB_2845279.
展开/折叠
CSHMT; Cytoplasmic serine hydroxymethyltransferase; cytosolic; GLYC_HUMAN; Glycine hydroxymethyltransferase; Serine hydroxymethyltransferase 1 (soluble); Serine hydroxymethyltransferase; Serine hydroxymethyltransferase, cytosolic; Serine methylase; SHMT; Shmt1;
抗原和靶标
- P34896 GLYC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTMPVNGAHKDADLWSSHDKMLAQPLKDSDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELETLCQKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINYDQLEENARLFHPKLIIAGTSCYSRNLEYARLRKIADENGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVKSVDPKTGKEILYNLESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTLEFKVYQHQVVANCRALSEALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASLFPLPGLPDF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P34896 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K10 | Acetylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| Y34 | Phosphorylation | Uniprot | |
| K38 | Sumoylation | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| K39 | Sumoylation | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| Y118 | Phosphorylation | Uniprot | |
| K157 | Acetylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K158 | Acetylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| Y180 | Phosphorylation | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K271 | Acetylation | Uniprot | |
| K274 | Acetylation | Uniprot | |
| K279 | Acetylation | Uniprot | |
| K375 | Acetylation | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| T405 | Phosphorylation | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| K449 | Ubiquitination | Uniprot | |
| K456 | Ubiquitination | Uniprot |
研究背景
Interconversion of serine and glycine.
Cytoplasm.
Homotetramer. Identified in complex with ABRAXAS2 and the other subunits of the BRISC complex, at least composed of ABRAXAS2, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1.
Belongs to the SHMT family.
研究领域
· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.
· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.
· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.
· Metabolism > Metabolism of cofactors and vitamins > One carbon pool by folate.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
限制条款
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