产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12479, RRID:AB_2845284.
展开/折叠
Signal recognition particle 54 kDa protein; Signal recognition particle 54kDa; SRP 54; SRP54; SRP54_HUMAN;
抗原和靶标
- P61011 SRP54_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLNKRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTFRAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVANAIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHIDDFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNEALIEKLKHGQFTLRDMYEQFQNIMKMGPFSQILGMIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKQPGRIQRVARGSGVSTRDVQELLTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMGGMAGLQSMMRQFQQGAAGNMKGMMGFNNM
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P61011 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R8 | Methylation | Uniprot | |
| T11 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| K33 | Acetylation | Uniprot | |
| K50 | Acetylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K81 | Acetylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| K321 | Ubiquitination | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| S451 | Phosphorylation | Uniprot | |
| S453 | Phosphorylation | Uniprot | |
| K457 | Ubiquitination | Uniprot | |
| K464 | Ubiquitination | Uniprot | |
| K496 | Methylation | Uniprot | |
| K496 | Ubiquitination | Uniprot |
研究背景
Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).
Nucleus speckle. Cytoplasm.
Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. Interacts with RNPS1.
Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.
Belongs to the GTP-binding SRP family. SRP54 subfamily.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein export.
限制条款
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