TRAPPC9 Antibody - #DF12489

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产品: TRAPPC9 抗体
货号: DF12489
描述: Rabbit polyclonal antibody to TRAPPC9
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Zebrafish
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 128-140 kDa; 129kD(Calculated).
蛋白号: Q96Q05
RRID: AB_2845294

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Zebrafish
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(83%), Rabbit(100%), Dog(100%), Chicken(83%)
克隆:
Polyclonal
特异性:
TRAPPC9 Antibody detects endogenous levels of total TRAPPC9.
RRID:
AB_2845294
引用格式: Affinity Biosciences Cat# DF12489, RRID:AB_2845294.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

1810044A24Rik; 2900005P22Rik; 4632408O18Rik; Ibp; IKBKBBP; Ikk2 binding protein; KIAA1882; MGC4737; MGC4769; mKIAA1882; MRT13; Nibp; NIK and IKK(beta) binding protein; NIK and IKK{beta} binding protein; RGD1309461; T1; Trafficking protein particle complex 9; TRAPP 120 kDa subunit; TRS130; Tularik gene 1 protein;

抗原和靶标

免疫原:
Uniprot:

Q96Q05(TPPC9_HUMAN) >>Visit HPA database.

基因/基因ID:
TRAPPC9(83696)
表达:
Q96Q05 TPPC9_HUMAN:

Expressed at high levels in muscle and kidney and to a lower extent in brain, heart and placenta.

序列:
MSVPDYMQCAEDHQTLLVVVQPVGIVSEENFFRIYKRICSVSQISVRDSQRVLYIRYRHHYPPENNEWGDFQTHRKVVGLITITDCFSAKDWPQTFEKFHVQKEIYGSTLYDSRLFVFGLQGEIVEQPRTDVAFYPNYEDCQTVEKRIEDFIESLFIVLESKRLDRATDKSGDKIPLLCVPFEKKDFVGLDTDSRHYKKRCQGRMRKHVGDLCLQAGMLQDSLVHYHMSVELLRSVNDFLWLGAALEGLCSASVIYHYPGGTGGKSGARRFQGSTLPAEAANRHRPGAQEVLIDPGALTTNGINPDTSTEIGRAKNCLSPEDIIDKYKEAISYYSKYKNAGVIELEACIKAVRVLAIQKRSMEASEFLQNAVYINLRQLSEEEKIQRYSILSELYELIGFHRKSAFFKRVAAMQCVAPSIAEPGWRACYKLLLETLPGYSLSLDPKDFSRGTHRGWAAVQMRLLHELVYASRRMGNPALSVRHLSFLLQTMLDFLSDQEKKDVAQSLENYTSKCPGTMEPIALPGGLTLPPVPFTKLPIVRHVKLLNLPASLRPHKMKSLLGQNVSTKSPFIYSPIIAHNRGEERNKKIDFQWVQGDVCEVQLMVYNPMPFELRVENMGLLTSGVEFESLPAALSLPAESGLYPVTLVGVPQTTGTITVNGYHTTVFGVFSDCLLDNLPGIKTSGSTVEVIPALPRLQISTSLPRSAHSLQPSSGDEISTNVSVQLYNGESQQLIIKLENIGMEPLEKLEVTSKVLTTKEKLYGDFLSWKLEETLAQFPLQPGKVATFTINIKVKLDFSCQENLLQDLSDDGISVSGFPLSSPFRQVVRPRVEGKPVNPPESNKAGDYSHVKTLEAVLNFKYSGGPGHTEGYYRNLSLGLHVEVEPSVFFTRVSTLPATSTRQCHLLLDVFNSTEHELTVSTRSSEALILHAGECQRMAIQVDKFNFESFPESPGEKGQFANPKQLEEERREARGLEIHSKLGICWRIPSLKRSGEASVEGLLNQLVLEHLQLAPLQWDVLVDGQPCDREAVAACQVGDPVRLEVRLTNRSPRSVGPFALTVVPFQDHQNGVHNYDLHDTVSFVGSSTFYLDAVQPSGQSACLGALLFLYTGDFFLHIRFHEDSTSKELPPSWFCLPSVHVCALEAQA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Sheep
83
Chicken
83
Xenopus
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q96Q05 作为底物

Site PTM Type Enzyme
S45 Phosphorylation
K98 Ubiquitination
K103 Ubiquitination
K146 Ubiquitination
K174 Ubiquitination
K185 Ubiquitination
S274 Phosphorylation
K315 Ubiquitination
K326 Ubiquitination
Y327 Phosphorylation
K328 Ubiquitination
Y334 Phosphorylation
K336 Ubiquitination
Y337 Phosphorylation
K359 Ubiquitination
K408 Acetylation
K446 Ubiquitination
Y469 Phosphorylation
S480 Phosphorylation
K501 Ubiquitination
K558 Ubiquitination
S559 Phosphorylation
S566 Phosphorylation
T567 Phosphorylation
K568 Ubiquitination
S569 Phosphorylation
Y573 Phosphorylation
S574 Phosphorylation
K748 Ubiquitination
S753 Phosphorylation
K754 Ubiquitination
T757 Phosphorylation
T758 Phosphorylation
K759 Ubiquitination
K761 Ubiquitination
K835 Ubiquitination
K944 Ubiquitination
S953 Phosphorylation
K957 Ubiquitination
K964 Ubiquitination

研究背景

功能:

Functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. May function in neuronal cells differentiation. May play a role in vesicular transport from endoplasmic reticulum to Golgi.

细胞定位:

Golgi apparatus>cis-Golgi network. Endoplasmic reticulum. Cytoplasm.
Note: Processes and cell bodies of neurons.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed at high levels in muscle and kidney and to a lower extent in brain, heart and placenta.

亚基结构:

Component of the multisubunit TRAPP (transport protein particle) complex, which includes at least TRAPPC2, TRAPPC2L, TRAPPC3, TRAPPC3L, TRAPPC4, TRAPPC5, TRAPPC8, TRAPPC9, TRAPPC10, TRAPPC11 and TRAPPC12. Directly interacts with IKBKB and MAP3K14.

蛋白家族:

Belongs to the NIBP family.

文献引用

1). Synaptic and Genetic Bases of Impaired Motor Learning Associated with Modified Experience-Dependent Cortical Plasticity in Heterozygous Reeler Mutants. Cerebral Cortex, 2022 (PubMed: 34339488) [IF=3.7]

Application: IF/ICC    Species: Mouse    Sample:

Figure 6. Gene enrichment of M1 in WT and HRM. (A) MA plots showing enriched genes in the comparative analyses of (Left) HRM-untrained versus WT-untrained, and (right) HRM-trained versus WT-trained group, identified by the RNA-seq (n = 3 mice). There was no gene enrichment in the comparative analysis of WT-trained versus WT-untrained, and HRM-trained versus HRM-untrained. (B) Left: gene expression levels of Smn1, measured by RNA-sequencing (n = 3 mice, ∗P < 0.05 Wald tests). Right: validation of the gene expression by qRT-PCR, normalized by the geometric mean of the expression levels of Actb and Rps18 (n = 6–8 mice, ∗∗P < 0.01, 1-way ANOVA Tukey’s post hoc tests). (C) Left: gene expression levels of Trappc9, measured by RNA-sequencing. (n = 3 mice, ∗∗P < 0.01 Wald tests). Right: validation of the gene expression by qRT-PCR (n = 8 mice, ∗∗∗P < 0.001, 1-way ANOVA Tukey’s post hoc tests). (D) Smn1 immunohistochemistry on frozen M1 sections, contralateral to the trained forepaw, of trained WT mouse and trained HRM mouse, showing less intensive and less extensive staining in both layers II/III and layer V of trained HRM mouse. Left: cortical layer I to V, scale bar = 100 μm; Middle: layer II/III, scale bar = 50 μm; Right: layer V, scale bar = 50 μm. (E) Trappc9 immunohistochemistry on frozen M1 sections of trained WT mouse and trained HRM mouse, showing less intensive staining in both layers II/III and layer V of trained HRM mouse

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