(1)
(3)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12492, RRID:AB_2845297.
展开/折叠
CHNG1; hTSHR I; hTSHRI; LGR 3; LGR3; MGC75129; Seven transmembrane helix receptor; Thyroid adenoma hyperfunctioning; Thyroid carcinoma with thyrotoxicosis; Thyroid stimulating hormone receptor; Thyroid stimulating hormone receptor, isoform 2; Thyroid-stimulating hormone receptor; Thyrotropin receptor; Thyrotropin receptor I; Thyrotropin receptor I, hTSHR I; TSH R; TSH-R; TSHR; TSHR_HUMAN;
抗原和靶标
Expressed in thyroide cells (at protein level) (PubMed:11847099). Expressed in the thyroid (PubMed:2610690).
- P16473 TSHR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRPADLLQLVLLLDLPRDLGGMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQTSVTALPSKGLEHLKELIARNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSMQSLRQRKSVNALNSPLHQEYEENLGDSIVGYKEKSKFQDTHNNAHYYVFFEEQEDEIIGFGQELKNPQEETLQAFDSHYDYTICGDSEDMVCTPKSDEFNPCEDIMGYKFLRIVVWFVSLLALLGNVFVLLILLTSHYKLNVPRFLMCNLAFADFCMGMYLLLIASVDLYTHSEYYNHAIDWQTGPGCNTAGFFTVFASELSVYTLTVITLERWYAITFAMRLDRKIRLRHACAIMVGGWVCCFLLALLPLVGISSYAKVSICLPMDTETPLALAYIVFVLTLNIVAFVIVCCCYVKIYITVRNPQYNPGDKDTKIAKRMAVLIFTDFICMAPISFYALSAILNKPLITVSNSKILLVLFYPLNSCANPFLYAIFTKAFQRDVFILLSKFGICKRQAQAYRGQRVPPKNSTDIQVQKVTHDMRQGLHNMEDVYELIENSHLTPKKQGQISEEYMQTVL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P16473 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K42 | Ubiquitination | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| N77 | N-Glycosylation | Uniprot | |
| N99 | N-Glycosylation | Uniprot | |
| N113 | N-Glycosylation | Uniprot | |
| N177 | N-Glycosylation | Uniprot | |
| N198 | N-Glycosylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| N302 | N-Glycosylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| Y706 | Phosphorylation | Uniprot | |
| Y739 | Phosphorylation | Uniprot | |
| S745 | Phosphorylation | Uniprot | |
| T748 | Phosphorylation | Uniprot |
研究背景
Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Plays a central role in controlling thyroid cell metabolism (By similarity).
Glycosylated.
Sulfated. Sulfation on Tyr-385 plays a role in thyrotropin receptor binding and activation.
Cell membrane>Multi-pass membrane protein. Basolateral cell membrane>Multi-pass membrane protein.
Expressed in thyroide cells (at protein level). Expressed in the thyroid.
Interacts with heterodimer GPHA2:GPHB5; this interaction stimulates cAMP production. Interacts (via the PDZ-binding motif) with SCRIB; regulates TSHR trafficking and function.
Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
研究领域
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
· Human Diseases > Immune diseases > Autoimmune thyroid disease.
· Organismal Systems > Endocrine system > Thyroid hormone synthesis.
· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.
文献引用
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