| 产品: | UBE2E2 抗体 |
| 货号: | DF12493 |
| 描述: | Rabbit polyclonal antibody to UBE2E2 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Zebrafish, Chicken, Xenopus |
| 分子量: | 22 kDa; 22kD(Calculated). |
| 蛋白号: | Q96LR5 |
| RRID: | AB_2845298 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12493, RRID:AB_2845298.
展开/折叠
FLJ25157; UB2E2_HUMAN; UBC4/5 homolog yeast; UBCH 8; UbcH8; Ube2e2; Ubiquitin carrier protein E2; Ubiquitin conjugating enzyme E2 E2; Ubiquitin conjugating enzyme E2E 2 (homologous to yeast UBC4/5); Ubiquitin conjugating enzyme E2E 2 (UBC4/5 homolog yeast); Ubiquitin conjugating enzyme E2E 2; Ubiquitin protein ligase E2; Ubiquitin-conjugating enzyme E2 E2; Ubiquitin-protein ligase E2;
抗原和靶标
- Q96LR5 UB2E2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTEAQRVDDSPSTSGGSSDGDQRESVQQEPEREQVQPKKKEGKISSKTAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96LR5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| T3 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K44 | Methylation | Uniprot | |
| K48 | Acetylation | Uniprot | |
| T49 | Phosphorylation | Uniprot | |
| K52 | Methylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| Y85 | Phosphorylation | Uniprot | |
| K144 | Ubiquitination | Uniprot |
研究背景
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein.
Autoubiquitinated in vitro.
Belongs to the ubiquitin-conjugating enzyme family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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