| 产品: | USP33 抗体 |
| 货号: | DF12497 |
| 描述: | Rabbit polyclonal antibody to USP33 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 103 kDa,107 kDa; 107kD(Calculated). |
| 蛋白号: | Q8TEY7 |
| RRID: | AB_2845302 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12497, RRID:AB_2845302.
展开/折叠
Deubiquitinating enzyme 33; EC 3 1 2 15; hVDU1; KIAA1097; MGC16868; OTTHUMP00000011234; OTTHUMP00000011330; OTTHUMP00000011331; pVHL interacting deubiquitinating enzyme 1; Ubiquitin carboxyl terminal hydrolase 33; Ubiquitin carboxyl-terminal hydrolase 33; Ubiquitin specific peptidase 33; Ubiquitin specific processing protease 33; ubiquitin specific protease 33; Ubiquitin thioesterase 33; Ubiquitin thiolesterase 33; Ubiquitin-specific-processing protease 33; UBP33_HUMAN; USP 33; Usp33; VDU 1; VDU1; VHL interacting deubiquitinating enzyme 1; VHL-interacting deubiquitinating enzyme 1;
抗原和靶标
- Q8TEY7 UBP33_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTGSNSHITILTLKVLPHFESLGKQEKIPNKMSAFRNHCPHLDSVGEITKEDLIQKSLGTCQDCKVQGPNLWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTQPSLPHVRQPHQIQENSVQDFKIPSNTTLKTPLVAVFDDLDIEADEEDELRARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPTTLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEVEEDPQTITTEETMEEDKSQSDVDFQSCESCSNSDRAENENGSRCFSEDNNETTMLIQDDENNSEMSKDWQKEKMCNKINKVNSEGEFDKDRDSISETVDLNNQETVKVQIHSRASEYITDVHSNDLSTPQILPSNEGVNPRLSASPPKSGNLWPGLAPPHKKAQSASPKRKKQHKKYRSVISDIFDGTIISSVQCLTCDRVSVTLETFQDLSLPIPGKEDLAKLHSSSHPTSIVKAGSCGEAYAPQGWIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQNFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQKERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGVPPRKAGYIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIEAEKIEKRRKTELEIFIRLNRAFQKEDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGNVMLRQGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDILQAEEKIEVETRSL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8TEY7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K50 | Ubiquitination | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K242 | Acetylation | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| T422 | Phosphorylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| Y588 | Phosphorylation | Uniprot | |
| K617 | Ubiquitination | Uniprot | |
| T668 | Phosphorylation | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| S671 | Phosphorylation | Uniprot | |
| K714 | Acetylation | Uniprot | |
| S727 | Phosphorylation | Uniprot | |
| S736 | Phosphorylation | Uniprot | |
| Y741 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | Uniprot | |
| K877 | Ubiquitination | Uniprot | |
| K882 | Ubiquitination | Uniprot | |
| S907 | Phosphorylation | Uniprot | |
| Y909 | Phosphorylation | Uniprot |
研究背景
Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.
Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Associates with centrosomes predominantly in S and G2 phases but less in G1 phase (PubMed:23486064).
Golgi apparatus.
Widely expressed.
Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with ARRB1, ARRB2, ADRB2, DIO2 and ROBO1. Interacts with SELENBP1; in a selenium-dependent manner. Interacts with CCP110.
The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 86 is replaced by a Glu residue.
Belongs to the peptidase C19 family. USP20/USP33 subfamily.
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