KLF4 Antibody - #AF2737

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产品: KLF4 抗体
货号: AF2737
描述: Rabbit polyclonal antibody to KLF4
应用: WB IHC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Rabbit, Dog
分子量: 50-60kDa; 55kD(Calculated).
蛋白号: O43474
RRID: AB_2845457

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
KLF4 Antibody detects endogenous levels of total KLF4.
RRID:
AB_2845457
引用格式: Affinity Biosciences Cat# AF2737, RRID:AB_2845457.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Endothelial Kruppel like zinc finger protein; Epithelial zinc finger protein EZF; EZF; GKLF; gut Kruppel-like factor; Gut-enriched krueppel-like factor; KLF; KLF4; KLF4_HUMAN; Krueppel-like factor 4; Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor); Kruppel like factor 4 (gut);

抗原和靶标

免疫原:
Uniprot:

O43474(KLF4_HUMAN) >>Visit HPA database.

基因/基因ID:
KLF4(9314)
序列:
MRQPPGESDMAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSHMKRLPPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQGQSRGFVARAGEPCVCWPHFGTHGMMLTPPSSPLELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
100
Pig
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O43474 作为底物

Site PTM Type Enzyme
K32 Ubiquitination
S234 Phosphorylation
S236 Phosphorylation
S240 Phosphorylation
S244 Phosphorylation
S254 Phosphorylation
S258 Phosphorylation
K278 Sumoylation
T316 Phosphorylation
S327 Phosphorylation
T429 Phosphorylation
S444 Phosphorylation
S445 Phosphorylation
K448 Sumoylation
K448 Ubiquitination
T453 Phosphorylation
T455 Phosphorylation

研究背景

功能:

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with POU5F1/OCT4 and SOX2 (By similarity). Interacts with MUC1 (via the C-terminal domain). Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a. Interacts with ZNF296 (By similarity). Interacts with GLIS1.

蛋白家族:

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the krueppel C2H2-type zinc-finger protein family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

文献引用

1). Artesunate inhibits macrophage-like phenotype switching of vascular smooth muscle cells and attenuates vascular inflammatory injury in atherosclerosis via NLRP3. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38325261) [IF=7.5]

Application: WB    Species: Human    Sample: HVSMCs

Fig. 7. Artesunate inhibits the inflammatory phenotypic transformation of HVSMCs and is related to NLRP3 inhibition. (A) Oil Red O staining of HVSMCs in vitro. (B) Immunofluorescence assay to determine the levels of α-SMA and CD68 in vitro. (C) Relative protein expression of KLF4 in vitro. (D) Immunofluorescence for α-SMA and CD68 expression in the aorta. (E) Immunohistochemistry for KLF4 expression in the aorta. ***P 
2). A Potent Protective Effect of Baicalein on Liver Injury by Regulating Mitochondria-Related Apoptosis. APOPTOSIS, 2020 (PubMed: 32409930) [IF=7.2]

Application: WB    Species: human    Sample: L02 cells

Fig. 5 |The protective mechanism of baicalein on oxidative stress induced hepatotoxicity. L02 cells were exposed to 100 μM H­2O2 for diferent indicated time. a The expression level of Drp1 and KLF4 were detected by western blotting and densitometry.
3). Succinate pretreatment attenuates intestinal ischemia-reperfusion injury by inhibiting necroptosis and inflammation via upregulating Klf4. International Immunopharmacology, 2023 (PubMed: 37285681) [IF=5.6]
4). Baicalein inhibits mitochondrial apoptosis induced by oxidative stress in cardiomyocytes by stabilizing MARCH5 expression. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020 (PubMed: 31880404) [IF=5.3]

Application: WB    Species: mouse    Sample: H9C2 cells

FIGURE 6|The cardioprotective pathway regulated by baicalein. H9C2 cells were exposed to 100μM H2O2 for 24 h after pre-treated with 50 μM baicalein for 4 h, transfected with MARCH5-cDNA or MARCH5-siRNA for 24 h, respectively. KLF4 and Drp1 proteins were detected by Western blotting. (A) The expression level of KLF4 and Drp1 in different time groups after treatment with H2O2

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