| 产品: | TRA2B 抗体 |
| 货号: | DF12519 |
| 描述: | Rabbit polyclonal antibody to TRA2B |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 34 kDa; 34kD(Calculated). |
| 蛋白号: | P62995 |
| RRID: | AB_2845481 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12519, RRID:AB_2845481.
展开/折叠
arginine/serine-rich 10; Arginine/serine-rich splicing factor 10; hTRA2-beta; SFRS10; Splicing factor; Splicing factor arginine/serine rich 10; SRFS10; TRA-2 beta; TRA2-beta; Tra2b; TRA2B_HUMAN; TRAN2B; Transformer 2 beta homolog; Transformer-2 protein homolog B; Transformer-2 protein homolog beta; Transformer-2-beta;
抗原和靶标
Highest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta and brain. Lowest expression in kidney and liver.
- P62995 TRA2B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDSGEQNYGERESRSASRSGSAHGSGKSARHTPARSRSKEDSRRSRSKSRSRSESRSRSRRSSRRHYTRSRSRSRSHRRSRSRSYSRDYRRRHSHSHSPMSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGRPTYGSSRRRDYYDRGYDRGYDDRDYYSRSYRGGGGGGGGWRAAQDRDQIYRRRSPSPYYSRGGYRSRSRSRSYSPRRY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P62995 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| T33 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| T103 | Phosphorylation | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| R187 | Methylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| T201 | Phosphorylation | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| R210 | Methylation | Uniprot | |
| T212 | Phosphorylation | Uniprot | |
| Y213 | Phosphorylation | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| Y221 | Phosphorylation | Uniprot | |
| Y222 | Phosphorylation | Uniprot | |
| R224 | Methylation | Uniprot | |
| Y226 | Phosphorylation | Uniprot | |
| R228 | Methylation | Uniprot | |
| Y230 | Phosphorylation | Uniprot | |
| R233 | Methylation | Uniprot | |
| Y235 | Phosphorylation | Uniprot | |
| Y236 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| R238 | Methylation | Uniprot | |
| Y240 | Phosphorylation | Uniprot | |
| R241 | Methylation | Uniprot | |
| R251 | Methylation | Uniprot | |
| R256 | Methylation | Uniprot | |
| Y260 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| Y268 | Phosphorylation | Uniprot | |
| Y269 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| R271 | Methylation | Uniprot | |
| Y274 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| Y283 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| Y288 | Phosphorylation | Uniprot |
研究背景
Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA.
Phosphorylated in the RS domains.
Dimethylation at Arg-241 is probably asymmetric.
Nucleus.
Highest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta and brain. Lowest expression in kidney and liver.
Interacts with SAFB/SAFB1 (By similarity). Found in a pre-mRNA exonic splicing enhancer (ESE) complex with TRA2B/SFRS10, SNRNP70, SNRPA1 and SRRM1. Binds to A3 enhancer proteins SFRS4, SFRS5, SFRS6 and SFRS9. Interacts with CPSF6, RBMY1A1, RBMX, RNPS1 and phosphorylated SFRS13A.
Belongs to the splicing factor SR family.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
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