| 产品: | APOB 抗体 |
| 货号: | DF12533 |
| 描述: | Rabbit polyclonal antibody to APOB |
| 应用: | WB |
| 反应: | Human, Mouse |
| 分子量: | 250 kDa; 516kD(Calculated). |
| 蛋白号: | P04114 |
| RRID: | AB_2845495 |
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse
克隆:
Polyclonal
特异性:
APOB Antibody detects endogenous levels of total APOB.
RRID:
AB_2845495
引用格式: Affinity Biosciences Cat# DF12533, RRID:AB_2845495.
引用格式: Affinity Biosciences Cat# DF12533, RRID:AB_2845495.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
Apo B-100; Apo B-48; ApoB 100; ApoB 48; APOB; APOB_HUMAN; Apolipoprotein B (including Ag(x) antigen); Apolipoprotein B 100; Apolipoprotein B; Apolipoprotein B-48; Apolipoprotein B48; FLDB; LDLCQ4;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
序列:
- P04114 APOB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDPPRPALLALLALPALLLLLLAGARAEEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSEEFAAAMSRYELKLAIPEGKQVFLYPEKDEPTYILNIKRGIISALLVPPETEEAKQVLFLDTVYGNCSTHFTVKTRKGNVATEISTERDLGQCDRFKPIRTGISPLALIKGMTRPLSTLISSSQSCQYTLDAKRKHVAEAICKEQHLFLPFSYKNKYGMVAQVTQTLKLEDTPKINSRFFGEGTKKMGLAFESTKSTSPPKQAEAVLKTLQELKKLTISEQNIQRANLFNKLVTELRGLSDEAVTSLLPQLIEVSSPITLQALVQCGQPQCSTHILQWLKRVHANPLLIDVVTYLVALIPEPSAQQLREIFNMARDQRSRATLYALSHAVNNYHKTNPTGTQELLDIANYLMEQIQDDCTGDEDYTYLILRVIGNMGQTMEQLTPELKSSILKCVQSTKPSLMIQKAAIQALRKMEPKDKDQEVLLQTFLDDASPGDKRLAAYLMLMRSPSQADINKIVQILPWEQNEQVKNFVASHIANILNSEELDIQDLKKLVKEALKESQLPTVMDFRKFSRNYQLYKSVSLPSLDPASAKIEGNLIFDPNNYLPKESMLKTTLTAFGFASADLIEIGLEGKGFEPTLEALFGKQGFFPDSVNKALYWVNGQVPDGVSKVLVDHFGYTKDDKHEQDMVNGIMLSVEKLIKDLKSKEVPEARAYLRILGEELGFASLHDLQLLGKLLLMGARTLQGIPQMIGEVIRKGSKNDFFLHYIFMENAFELPTGAGLQLQISSSGVIAPGAKAGVKLEVANMQAELVAKPSVSVEFVTNMGIIIPDFARSGVQMNTNFFHESGLEAHVALKAGKLKFIIPSPKRPVKLLSGGNTLHLVSTTKTEVIPPLIENRQSWSVCKQVFPGLNYCTSGAYSNASSTDSASYYPLTGDTRLELELRPTGEIEQYSVSATYELQREDRALVDTLKFVTQAEGAKQTEATMTFKYNRQSMTLSSEVQIPDFDVDLGTILRVNDESTEGKTSYRLTLDIQNKKITEVALMGHLSCDTKEERKIKGVISIPRLQAEARSEILAHWSPAKLLLQMDSSATAYGSTVSKRVAWHYDEEKIEFEWNTGTNVDTKKMTSNFPVDLSDYPKSLHMYANRLLDHRVPQTDMTFRHVGSKLIVAMSSWLQKASGSLPYTQTLQDHLNSLKEFNLQNMGLPDFHIPENLFLKSDGRVKYTLNKNSLKIEIPLPFGGKSSRDLKMLETVRTPALHFKSVGFHLPSREFQVPTFTIPKLYQLQVPLLGVLDLSTNVYSNLYNWSASYSGGNTSTDHFSLRARYHMKADSVVDLLSYNVQGSGETTYDHKNTFTLSYDGSLRHKFLDSNIKFSHVEKLGNNPVSKGLLIFDASSSWGPQMSASVHLDSKKKQHLFVKEVKIDGQFRVSSFYAKGTYGLSCQRDPNTGRLNGESNLRFNSSYLQGTNQITGRYEDGTLSLTSTSDLQSGIIKNTASLKYENYELTLKSDTNGKYKNFATSNKMDMTFSKQNALLRSEYQADYESLRFFSLLSGSLNSHGLELNADILGTDKINSGAHKATLRIGQDGISTSATTNLKCSLLVLENELNAELGLSGASMKLTTNGRFREHNAKFSLDGKAALTELSLGSAYQAMILGVDSKNIFNFKVSQEGLKLSNDMMGSYAEMKFDHTNSLNIAGLSLDFSSKLDNIYSSDKFYKQTVNLQLQPYSLVTTLNSDLKYNALDLTNNGKLRLEPLKLHVAGNLKGAYQNNEIKHIYAISSAALSASYKADTVAKVQGVEFSHRLNTDIAGLASAIDMSTNYNSDSLHFSNVFRSVMAPFTMTIDAHTNGNGKLALWGEHTGQLYSKFLLKAEPLAFTFSHDYKGSTSHHLVSRKSISAALEHKVSALLTPAEQTGTWKLKTQFNNNEYSQDLDAYNTKDKIGVELTGRTLADLTLLDSPIKVPLLLSEPINIIDALEMRDAVEKPQEFTIVAFVKYDKNQDVHSINLPFFETLQEYFERNRQTIIVVLENVQRNLKHINIDQFVRKYRAALGKLPQQANDYLNSFNWERQVSHAKEKLTALTKKYRITENDIQIALDDAKINFNEKLSQLQTYMIQFDQYIKDSYDLHDLKIAIANIIDEIIEKLKSLDEHYHIRVNLVKTIHDLHLFIENIDFNKSGSSTASWIQNVDTKYQIRIQIQEKLQQLKRHIQNIDIQHLAGKLKQHIEAIDVRVLLDQLGTTISFERINDILEHVKHFVINLIGDFEVAEKINAFRAKVHELIERYEVDQQIQVLMDKLVELAHQYKLKETIQKLSNVLQQVKIKDYFEKLVGFIDDAVKKLNELSFKTFIEDVNKFLDMLIKKLKSFDYHQFVDETNDKIREVTQRLNGEIQALELPQKAEALKLFLEETKATVAVYLESLQDTKITLIINWLQEALSSASLAHMKAKFRETLEDTRDRMYQMDIQQELQRYLSLVGQVYSTLVTYISDWWTLAAKNLTDFAEQYSIQDWAKRMKALVEQGFTVPEIKTILGTMPAFEVSLQALQKATFQTPDFIVPLTDLRIPSVQINFKDLKNIKIPSRFSTPEFTILNTFHIPSFTIDFVEMKVKIIRTIDQMLNSELQWPVPDIYLRDLKVEDIPLARITLPDFRLPEIAIPEFIIPTLNLNDFQVPDLHIPEFQLPHISHTIEVPTFGKLYSILKIQSPLFTLDANADIGNGTTSANEAGIAASITAKGESKLEVLNFDFQANAQLSNPKINPLALKESVKFSSKYLRTEHGSEMLFFGNAIEGKSNTVASLHTEKNTLELSNGVIVKINNQLTLDSNTKYFHKLNIPKLDFSSQADLRNEIKTLLKAGHIAWTSSGKGSWKWACPRFSDEGTHESQISFTIEGPLTSFGLSNKINSKHLRVNQNLVYESGSLNFSKLEIQSQVDSQHVGHSVLTAKGMALFGEGKAEFTGRHDAHLNGKVIGTLKNSLFFSAQPFEITASTNNEGNLKVRFPLRLTGKIDFLNNYALFLSPSAQQASWQVSARFNQYKYNQNFSAGNNENIMEAHVGINGEANLDFLNIPLTIPEMRLPYTIITTPPLKDFSLWEKTGLKEFLKTTKQSFDLSVKAQYKKNKHRHSITNPLAVLCEFISQSIKSFDRHFEKNRNNALDFVTKSYNETKIKFDKYKAEKSHDELPRTFQIPGYTVPVVNVEVSPFTIEMSAFGYVFPKAVSMPSFSILGSDVRVPSYTLILPSLELPVLHVPRNLKLSLPDFKELCTISHIFIPAMGNITYDFSFKSSVITLNTNAELFNQSDIVAHLLSSSSSVIDALQYKLEGTTRLTRKRGLKLATALSLSNKFVEGSHNSTVSLTTKNMEVSVATTTKAQIPILRMNFKQELNGNTKSKPTVSSSMEFKYDFNSSMLYSTAKGAVDHKLSLESLTSYFSIESSTKGDVKGSVLSREYSGTIASEANTYLNSKSTRSSVKLQGTSKIDDIWNLEVKENFAGEATLQRIYSLWEHSTKNHLQLEGLFFTNGEHTSKATLELSPWQMSALVQVHASQPSSFHDFPDLGQEVALNANTKNQKIRWKNEVRIHSGSFQSQVELSNDQEKAHLDIAGSLEGHLRFLKNIILPVYDKSLWDFLKLDVTTSIGRRQHLRVSTAFVYTKNPNGYSFSIPVKVLADKFIIPGLKLNDLNSVLVMPTFHVPFTDLQVPSCKLDFREIQIYKKLRTSSFALNLPTLPEVKFPEVDVLTKYSQPEDSLIPFFEITVPESQLTVSQFTLPKSVSDGIAALDLNAVANKIADFELPTIIVPEQTIEIPSIKFSVPAGIVIPSFQALTARFEVDSPVYNATWSASLKNKADYVETVLDSTCSSTVQFLEYELNVLGTHKIEDGTLASKTKGTFAHRDFSAEYEEDGKYEGLQEWEGKAHLNIKSPAFTDLHLRYQKDKKGISTSAASPAVGTVGMDMDEDDDFSKWNFYYSPQSSPDKKLTIFKTELRVRESDEETQIKVNWEEEAASGLLTSLKDNVPKATGVLYDYVNKYHWEHTGLTLREVSSKLRRNLQNNAEWVYQGAIRQIDDIDVRFQKAASGTTGTYQEWKDKAQNLYQELLTQEGQASFQGLKDNVFDGLVRVTQEFHMKVKHLIDSLIDFLNFPRFQFPGKPGIYTREELCTMFIREVGTVLSQVYSKVHNGSEILFSYFQDLVITLPFELRKHKLIDVISMYRELLKDLSKEAQEVFKAIQSLKTTEVLRNLQDLLQFIFQLIEDNIKQLKEMKFTYLINYIQDEINTIFSDYIPYVFKLLKENLCLNLHKFNEFIQNELQEASQELQQIHQYIMALREEYFDPSIVGWTVKYYELEEKIVSLIKNLLVALKDFHSEYIVSASNFTSQLSSQVEQFLHRNIQEYLSILTDPDGKGKEKIAELSATAQEIIKSQAIATKKIISDYHQQFRYKLQDFSDQLSDYYEKFIAESKRLIDLSIQNYHTFLIYITELLKKLQSTTVMNPYMKLAPGELTIIL
翻译修饰 - P04114 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Acetylation | Uniprot | ||
| Y52 | Phosphorylation | Uniprot | |
| Y129 | Phosphorylation | Uniprot | |
| N185 | N-Glycosylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| K252 | Acetylation | Uniprot | |
| Y276 | Phosphorylation | Uniprot | |
| T285 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| Y562 | Phosphorylation | Uniprot | |
| R567 | Methylation | Uniprot | |
| K612 | Acetylation | Uniprot | |
| Y740 | Phosphorylation | Uniprot | |
| T741 | Phosphorylation | Uniprot | |
| S767 | Phosphorylation | Uniprot | |
| S928 | Phosphorylation | Uniprot | |
| T1102 | Phosphorylation | Uniprot | |
| T1114 | Phosphorylation | Uniprot | |
| S1135 | Phosphorylation | Uniprot | |
| Y1157 | Phosphorylation | Uniprot | |
| Y1169 | Phosphorylation | Uniprot | |
| T1190 | Phosphorylation | Uniprot | |
| S1191 | Phosphorylation | Uniprot | |
| S1198 | Phosphorylation | Uniprot | |
| Y1200 | Phosphorylation | Uniprot | |
| S1235 | Phosphorylation | Uniprot | |
| Y1287 | Phosphorylation | Uniprot | |
| T1288 | Phosphorylation | Uniprot | |
| N1523 | N-Glycosylation | Uniprot | |
| S1709 | Phosphorylation | Uniprot | |
| Y1714 | Phosphorylation | Uniprot | |
| S1723 | Phosphorylation | Uniprot | |
| Y1774 | Phosphorylation | Uniprot | |
| S1775 | Phosphorylation | Uniprot | |
| K1778 | Acetylation | Uniprot | |
| Y1840 | Phosphorylation | Uniprot | |
| Y1946 | Phosphorylation | Uniprot | |
| K2004 | Acetylation | Uniprot | |
| T2013 | Phosphorylation | Uniprot | |
| T2018 | Phosphorylation | Uniprot | |
| S2022 | Phosphorylation | Uniprot | |
| S2211 | Phosphorylation | Uniprot | |
| N2239 | N-Glycosylation | Uniprot | |
| K2265 | Acetylation | Uniprot | |
| Y2480 | Phosphorylation | Uniprot | |
| T2515 | Phosphorylation | Uniprot | |
| S2603 | Phosphorylation | Uniprot | |
| T2707 | Phosphorylation | Uniprot | |
| N2779 | N-Glycosylation | Uniprot | |
| Y2834 | Phosphorylation | Uniprot | |
| S2859 | Phosphorylation | Uniprot | |
| T2862 | Phosphorylation | Uniprot | |
| K2911 | Ubiquitination | Uniprot | |
| K2915 | Ubiquitination | Uniprot | |
| K2926 | Ubiquitination | Uniprot | |
| N2982 | N-Glycosylation | Uniprot | |
| N3101 | N-Glycosylation | Uniprot | |
| S3185 | Phosphorylation | Uniprot | |
| S3198 | Phosphorylation | Uniprot | |
| T3220 | O-Glycosylation | Uniprot | |
| N3224 | N-Glycosylation | Uniprot | |
| S3279 | Phosphorylation | Uniprot | |
| S3282 | Phosphorylation | Uniprot | |
| S3288 | Phosphorylation | Uniprot | |
| N3358 | N-Glycosylation | Uniprot | |
| T3397 | Phosphorylation | Uniprot | |
| S3400 | Phosphorylation | Uniprot | |
| S3402 | Phosphorylation | Uniprot | |
| N3411 | N-Glycosylation | Uniprot | |
| N3465 | N-Glycosylation | Uniprot | |
| T3472 | Phosphorylation | Uniprot | |
| T3626 | Phosphorylation | Uniprot | |
| S3643 | Phosphorylation | Uniprot | |
| S3646 | Phosphorylation | Uniprot | |
| Y3680 | Phosphorylation | Uniprot | |
| T3776 | Phosphorylation | Uniprot | |
| S3778 | Phosphorylation | Uniprot | |
| T3785 | Phosphorylation | Uniprot | |
| N3895 | N-Glycosylation | Uniprot | |
| Y3958 | Phosphorylation | Uniprot | |
| S4000 | Phosphorylation | Uniprot | |
| Y4025 | Phosphorylation | Uniprot | |
| Y4026 | Phosphorylation | Uniprot | |
| S4027 | Phosphorylation | Uniprot | |
| S4030 | Phosphorylation | Uniprot | |
| K4040 | Acetylation | Uniprot | |
| T4041 | Phosphorylation | Uniprot | |
| S4048 | Phosphorylation | Uniprot | |
| T4052 | Phosphorylation | Uniprot | |
| Y4116 | Phosphorylation | Uniprot | |
| T4140 | Phosphorylation | Uniprot | |
| S4229 | Phosphorylation | Uniprot | |
| S4409 | Phosphorylation | Uniprot | |
| S4507 | Phosphorylation | Uniprot | |
| Y4510 | Phosphorylation | Uniprot |
研究背景
功能:
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
翻译修饰:
Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
细胞定位:
Cytoplasm. Secreted.
亚基结构:
Interacts with PCSK9. Interacts with MTTP.
研究领域
· Organismal Systems > Digestive system > Fat digestion and absorption.
· Organismal Systems > Digestive system > Vitamin digestion and absorption.
· Organismal Systems > Digestive system > Cholesterol metabolism.
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