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| 产品: | M-CSF 抗体 |
| 货号: | DF12536 |
| 描述: | Rabbit polyclonal antibody to M-CSF |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Sheep, Rabbit |
| 分子量: | 70 kDa; 60kD(Calculated). |
| 蛋白号: | P09603 |
| RRID: | AB_2845498 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12536, RRID:AB_2845498.
展开/折叠
Colony stimulating factor 1 (macrophage); Colony stimulating factor 1; Colony stimulating factor macrophage specific; CSF 1; CSF-1; CSF1; CSF1_HUMAN; Csfm; Lanimostim; M CSF; M-CSF; Macrophage Colony Stimulating Factor 1; Macrophage colony stimulating factor; MCSF; MGC31930; Processed macrophage colony-stimulating factor 1;
抗原和靶标
- P09603 CSF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTAPGAAGRCPPTTWLGSLLLLVCLLASRSITEEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQDVVTKPDCNCLYPKAIPSSDPASVSPHQPLAPSMAPVAGLTWEDSEGTEGSSLLPGEQPLHTVDPGSAKQRPPRSTCQSFEPPETPVVKDSTIGGSPQPRPSVGAFNPGMEDILDSAMGTNWVPEEASGEASEIPVPQGTELSPSRPGGGSMQTEPARPSNFLSASSPLPASAKGQQPADVTGTALPRVGPVRPTGQDWNHTPQKTDHPSALLRDPPEPGSPRISSLRPQGLSNPSTLSAQPQLSRSHSSGSVLPLGELEGRRSTRDRRSPAEPEGGPASEGAARPLPRFNSVPLTDTGHERQSEGSFSPQLQESVFHLLVPSVILVLLAVGGLLFYRWRRRSHQEPQRADSPLEQPEGSPLTQDDRQVELPV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P09603 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S256 | O-Glycosylation | Uniprot | |
| T257 | O-Glycosylation | Uniprot | |
| T266 | O-Glycosylation | Uniprot | |
| T266 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| T363 | O-Glycosylation | Uniprot | |
| T365 | O-Glycosylation | Uniprot | |
| T376 | O-Glycosylation | Uniprot | |
| T383 | O-Glycosylation | Uniprot | |
| T387 | O-Glycosylation | Uniprot | |
| S391 | O-Glycosylation | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| S426 | Phosphorylation | Uniprot | |
| S428 | O-Glycosylation | Uniprot | |
| S430 | O-Glycosylation | Uniprot | |
| S431 | O-Glycosylation | Uniprot | |
| S433 | O-Glycosylation | Uniprot | |
| S451 | O-Glycosylation | Uniprot | |
| S461 | O-Glycosylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| S473 | O-Glycosylation | Uniprot | |
| T477 | O-Glycosylation | Uniprot | |
| S533 | Phosphorylation | Uniprot |
研究背景
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
N- and O-glycosylated. Glycosylation and proteolytic cleavage yield different soluble forms. One high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1 is N- and O-glycosylated. Isoform 3 is only N-glycosylated.
Cell membrane>Single-pass type I membrane protein.
Secreted>Extracellular space.
Homodimer or heterodimer; disulfide-linked. Interacts with CSF1R.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Human Diseases > Immune diseases > Rheumatoid arthritis.
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)
文献引用
Application: WB Species: Human Sample: SaOS-2 cells
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