| 产品: | AMOTL1 抗体 |
| 货号: | DF12556 |
| 描述: | Rabbit polyclonal antibody to AMOTL1 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Horse, Rabbit, Dog |
| 分子量: | 107 kDa; 107kD(Calculated). |
| 蛋白号: | Q8IY63 |
| RRID: | AB_2845518 |
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
预测:
Pig(82%), Horse(91%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
AMOTL1 Antibody detects endogenous levels of total AMOTL1.
RRID:
AB_2845518
引用格式: Affinity Biosciences Cat# DF12556, RRID:AB_2845518.
引用格式: Affinity Biosciences Cat# DF12556, RRID:AB_2845518.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
2310010G08Rik; 2310067L22Rik; 4932416D09Rik; AMOL1_HUMAN; Amotl1; Angiomotin-like protein 1; FLJ00155; JEAP; Junction enriched and associated protein; mFLJ00155;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
序列:
- Q8IY63 AMOL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MWRAKLRRGTCEPAVKGSPSACYSPSSPVQVLEDSTYFSPDFQLYSGRHETSALTVEATSSIREKVVEDPLCNFHSPNFLRISEVEMRGSEDAAAGTVLQRLIQEQLRYGTPTENMNLLAIQHQATGSAGPAHPTNNFSSTENLTQEDPQMVYQSARQEPQGQEHQVDNTVMEKQVRSTQPQQNNEELPTYEEAKAQSQFFRGQQQQQQQQGAVGHGYYMAGGTSQKSRTEGRPTVNRANSGQAHKDEALKELKQGHVRSLSERIMQLSLERNGAKQHLPGSGNGKGFKVGGGPSPAQPAGKVLDPRGPPPEYPFKTKQMMSPVSKTQEHGLFYGDQHPGMLHEMVKPYPAPQPVRTDVAVLRYQPPPEYGVTSRPCQLPFPSTMQQHSPMSSQTSSASGPLHSVSLPLPLPMALGAPQPPPAASPSQQLGPDAFAIVERAQQMVEILTEENRVLHQELQGYYDNADKLHKFEKELQRISEAYESLVKSTTKRESLDKAMRNKLEGEIRRLHDFNRDLRDRLETANRQLSSREYEGHEDKAAEGHYASQNKEFLKEKEKLEMELAAVRTASEDHRRHIEILDQALSNAQARVIKLEEELREKQAYVEKVEKLQQALTQLQSACEKREQMERRLRTWLERELDALRTQQKHGNGQPANMPEYNAPALLELVREKEERILALEADMTKWEQKYLEESTIRHFAMNAAATAAAERDTTIINHSRNGSYGESSLEAHIWQEEEEVVQANRRCQDMEYTIKNLHAKIIEKDAMIKVLQQRSRKDAGKTDSSSLRPARSVPSIAAATGTHSRQTSLTSSQLAEEKKEEKTWKGSIGLLLGKEHHEHASAPLLPPPPTSALSSIASTTAASSAHAKTGSKDSSTQTDKSAELFWPSMASLPSRGRLSTTPAHSPVLKHPAAKGTAEKLENSPGHGKSPDHRGRVSSLLHKPEFPDGEMMEVLI
种属预测
种属预测:
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
Species
Results
Score
Rabbit
100
Dog
100
Horse
91
Pig
82
Bovine
73
Sheep
73
Chicken
73
Xenopus
60
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8IY63 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K16 | Ubiquitination | Uniprot | |
| T51 | Phosphorylation | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| Y191 | Phosphorylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| Y218 | Phosphorylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| K316 | Methylation | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| K318 | Methylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| T373 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| K503 | Ubiquitination | Uniprot | |
| K540 | Ubiquitination | Uniprot | |
| Y546 | Phosphorylation | Uniprot | |
| K551 | Ubiquitination | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| K611 | Ubiquitination | Uniprot | |
| K625 | Ubiquitination | Uniprot | |
| K649 | Ubiquitination | Uniprot | |
| Y661 | Phosphorylation | Uniprot | |
| K686 | Ubiquitination | Uniprot | |
| K690 | Ubiquitination | Uniprot | |
| T707 | Phosphorylation | Uniprot | |
| T714 | Phosphorylation | Uniprot | |
| T715 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S724 | Phosphorylation | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| K761 | Ubiquitination | Uniprot | |
| K765 | Ubiquitination | Uniprot | |
| K782 | Ubiquitination | Uniprot | |
| S793 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
| S796 | Phosphorylation | Uniprot | |
| T803 | Phosphorylation | Uniprot | |
| S805 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| S813 | Phosphorylation | Uniprot | |
| S828 | Phosphorylation | Uniprot | |
| S876 | Phosphorylation | Uniprot | |
| T877 | Phosphorylation | Uniprot | |
| T879 | Phosphorylation | Uniprot | |
| S892 | Phosphorylation | Uniprot | |
| S895 | Phosphorylation | Uniprot | |
| S900 | Phosphorylation | Uniprot | |
| T901 | Phosphorylation | Uniprot | |
| T902 | Phosphorylation | Uniprot | |
| S906 | Phosphorylation | Uniprot | |
| T917 | Phosphorylation | Uniprot | |
| S924 | Phosphorylation | Uniprot | |
| S930 | Phosphorylation | Uniprot | |
| S939 | Phosphorylation | Uniprot |
研究背景
功能:
Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus.
翻译修饰:
Polyubiquitinated by NEDD4, leading to proteasomal degradation.
细胞定位:
Cell junction>Tight junction.
蛋白家族:
Belongs to the angiomotin family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
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