BNIP1 Antibody - #DF12569

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产品: BNIP1 抗体
货号: DF12569
描述: Rabbit polyclonal antibody to BNIP1
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 26 kDa; 26kD(Calculated).
蛋白号: Q12981
RRID: AB_2845531

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(92%), Bovine(83%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(100%), Xenopus(83%)
克隆:
Polyclonal
特异性:
BNIP1 Antibody detects endogenous levels of total BNIP1.
RRID:
AB_2845531
引用格式: Affinity Biosciences Cat# DF12569, RRID:AB_2845531.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BCL2 adenovirus E1B 19kD interacting protein 1; BCL2/adenovirus E1B 19 kDa protein-interacting protein 1; BNIP1; MGC41600; Nip1; OTTHUMP00000161077; OTTHUMP00000161078; OTTHUMP00000161079; OTTHUMP00000223580; sec20; SEC20_HUMAN; SEC20L; Transformation-related gene 8 protein; TRG-8; TRG8; Vesicle transport protein SEC20;

抗原和靶标

免疫原:
Uniprot:

Q12981(SEC20_HUMAN) >>Visit HPA database.

基因/基因ID:
BNIP1(662)
表达:
Q12981 SEC20_HUMAN:

Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine.

序列:
MAAPQDVHVRICNQEIVKFDLEVKALIQDIRDCSGPLSALTELNTKVKEKFQQLRHRIQDLEQLAKEQDKESEKQLLLQEVENHKKQMLSNQASWRKANLTCKIAIDNLEKAELLQGGDLLRQRKTTKESLAQTSSTITESLMGISRMMAQQVQQSEEAMQSLVTSSRTILDANEEFKSMSGTIQLGRKLITKYNRRELTDKLLIFLALALFLATVLYIVKKRLFPFL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Chicken
100
Pig
92
Horse
92
Sheep
92
Dog
92
Rabbit
92
Bovine
83
Xenopus
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q12981 作为底物

Site PTM Type Enzyme
K46 Acetylation
K46 Ubiquitination
K48 Acetylation
K48 Methylation
K50 Methylation
K50 Ubiquitination
K66 Ubiquitination
K74 Ubiquitination
K86 Ubiquitination
S90 Phosphorylation
S94 Phosphorylation
K103 Ubiquitination
K111 Ubiquitination
T126 Phosphorylation
T127 Phosphorylation
K128 Ubiquitination
K178 Ubiquitination
S181 Phosphorylation
K189 Acetylation
K193 Acetylation
T200 Phosphorylation
T215 Phosphorylation
Y218 Phosphorylation

研究背景

功能:

As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization. Plays also a role in apoptosis. It is for instance required for endoplasmic reticulum stress-induced apoptosis. As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy (Probable).

翻译修饰:

Polyubiquitinated. 'Lys-63'-linked polyubiquitination by RNF185 increases the interaction with the autophagy receptor SQSTM1. Undergoes 'Lys-29'- and 'Lys-63'-linked polyubiquitination by RNF186 that may regulate BNIP1 localization to the mitochondrion.

细胞定位:

Endoplasmic reticulum membrane>Single-pass type IV membrane protein. Mitochondrion membrane>Single-pass type IV membrane protein.
Note: Localization to the mitochondrion is regulated by RNF186.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine.

亚基结构:

Component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B. Interacts directly with STX18, RINT1/TIP20L and NAPA. Interacts with ZW10 through RINT1. Interacts with BCL2. Interacts with RNF186. Interacts with RNF185. Interacts with SQSTM1; increased by 'Lys-63'-linked polyubiquitination of BNIP1.

(Microbial infection) Interacts with adenovirus E1B 19K protein; plays a role in the suppression of cell apoptosis by the viral protein.

蛋白家族:

Belongs to the SEC20 family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

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