| 产品: | BNIP1 抗体 |
| 货号: | DF12569 |
| 描述: | Rabbit polyclonal antibody to BNIP1 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 26 kDa; 26kD(Calculated). |
| 蛋白号: | Q12981 |
| RRID: | AB_2845531 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12569, RRID:AB_2845531.
展开/折叠
BCL2 adenovirus E1B 19kD interacting protein 1; BCL2/adenovirus E1B 19 kDa protein-interacting protein 1; BNIP1; MGC41600; Nip1; OTTHUMP00000161077; OTTHUMP00000161078; OTTHUMP00000161079; OTTHUMP00000223580; sec20; SEC20_HUMAN; SEC20L; Transformation-related gene 8 protein; TRG-8; TRG8; Vesicle transport protein SEC20;
抗原和靶标
Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine.
- Q12981 SEC20_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAPQDVHVRICNQEIVKFDLEVKALIQDIRDCSGPLSALTELNTKVKEKFQQLRHRIQDLEQLAKEQDKESEKQLLLQEVENHKKQMLSNQASWRKANLTCKIAIDNLEKAELLQGGDLLRQRKTTKESLAQTSSTITESLMGISRMMAQQVQQSEEAMQSLVTSSRTILDANEEFKSMSGTIQLGRKLITKYNRRELTDKLLIFLALALFLATVLYIVKKRLFPFL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q12981 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K46 | Acetylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| K48 | Acetylation | Uniprot | |
| K48 | Methylation | Uniprot | |
| K50 | Methylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| T126 | Phosphorylation | Uniprot | |
| T127 | Phosphorylation | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| K189 | Acetylation | Uniprot | |
| K193 | Acetylation | Uniprot | |
| T200 | Phosphorylation | Uniprot | |
| T215 | Phosphorylation | Uniprot | |
| Y218 | Phosphorylation | Uniprot |
研究背景
As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization. Plays also a role in apoptosis. It is for instance required for endoplasmic reticulum stress-induced apoptosis. As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy (Probable).
Polyubiquitinated. 'Lys-63'-linked polyubiquitination by RNF185 increases the interaction with the autophagy receptor SQSTM1. Undergoes 'Lys-29'- and 'Lys-63'-linked polyubiquitination by RNF186 that may regulate BNIP1 localization to the mitochondrion.
Endoplasmic reticulum membrane>Single-pass type IV membrane protein. Mitochondrion membrane>Single-pass type IV membrane protein.
Note: Localization to the mitochondrion is regulated by RNF186.
Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine.
Component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B. Interacts directly with STX18, RINT1/TIP20L and NAPA. Interacts with ZW10 through RINT1. Interacts with BCL2. Interacts with RNF186. Interacts with RNF185. Interacts with SQSTM1; increased by 'Lys-63'-linked polyubiquitination of BNIP1.
(Microbial infection) Interacts with adenovirus E1B 19K protein; plays a role in the suppression of cell apoptosis by the viral protein.
Belongs to the SEC20 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.