| 产品: | CNOT3 抗体 |
| 货号: | DF12583 |
| 描述: | Rabbit polyclonal antibody to CNOT3 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Rabbit, Dog, Xenopus |
| 分子量: | 120 kDa; 82kD(Calculated). |
| 蛋白号: | O75175 |
| RRID: | AB_2845545 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12583, RRID:AB_2845545.
展开/折叠
CCR4 associated factor 3; CCR4-associated factor 3; CCR4-NOT transcription complex subunit 3; CNOT3; CNOT3_HUMAN; KIAA0691; LENG2; Leukocyte receptor cluster member 2; negative regulator of transcription 3, S. cerevisiae, homolog of; NOT3 (negative regulator of transcription 3, yeast) homolog; NOT3; NOT3H;
抗原和靶标
Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
- O75175 CNOT3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADKRKLQGEIDRCLKKVSEGVEQFEDIWQKLHNAANANQKEKYEADLKKEIKKLQRLRDQIKTWVASNEIKDKRQLIDNRKLIETQMERFKVVERETKTKAYSKEGLGLAQKVDPAQKEKEEVGQWLTNTIDTLNMQVDQFESEVESLSVQTRKKKGDKDKQDRIEGLKRHIEKHRYHVRMLETILRMLDNDSILVDAIRKIKDDVEYYVDSSQDPDFEENEFLYDDLDLEDIPQALVATSPPSHSHMEDEIFNQSSSTPTSTTSSSPIPPSPANCTTENSEDDKKRGRSTDSEVSQSPAKNGSKPVHSNQHPQSPAVPPTYPSGPPPAASALSTTPGNNGVPAPAAPPSALGPKASPAPSHNSGTPAPYAQAVAPPAPSGPSTTQPRPPSVQPSGGGGGGSGGGGSSSSSNSSAGGGAGKQNGATSYSSVVADSPAEVALSSSGGNNASSQALGPPSGPHNPPPSTSKEPSAAAPTGAGGVAPGSGNNSGGPSLLVPLPVNPPSSPTPSFSDAKAAGALLNGPPQFSTAPEIKAPEPLSSLKSMAERAAISSGIEDPVPTLHLTERDIILSSTSAPPASAQPPLQLSEVNIPLSLGVCPLGPVPLTKEQLYQQAMEEAAWHHMPHPSDSERIRQYLPRNPCPTPPYHHQMPPPHSDTVEFYQRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHEEPKTITDEFEQGTYIYFDYEKWGQRKKEGFTFEYRYLEDRDLQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75175 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K41 | Ubiquitination | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| T100 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| T292 | Phosphorylation | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| S506 | Phosphorylation | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| S541 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| R635 | Methylation | Uniprot | |
| T645 | Phosphorylation | Uniprot | |
| Y685 | Phosphorylation | Uniprot | |
| K689 | Ubiquitination | Uniprot | |
| K737 | Ubiquitination | Uniprot |
研究背景
Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity.
Cytoplasm. Nucleus. Cytoplasm>P-body.
Note: NANOS2 promotes its localization to P-body.
Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT2. Interacts with TIP120B and NANOS2.
Belongs to the CNOT2/3/5 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
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