| 产品: | DBNL 抗体 |
| 货号: | DF12594 |
| 描述: | Rabbit polyclonal antibody to DBNL |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 55 kDa; 48kD(Calculated). |
| 蛋白号: | Q9UJU6 |
| RRID: | AB_2845556 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12594, RRID:AB_2845556.
展开/折叠
ABP1; Actin-binding protein 1; Cervical mucin associated protein; Cervical mucin-associated protein; Cervical SH3P7; CMAP; Dbnl; DBNL_HUMAN; Drebrin F; drebrin like; Drebrin like protein; Drebrin-F; Drebrin-like protein; HIP 55; HIP-55; HPK1 interacting protein of 55 kDa; HPK1-interacting protein of 55 kDa; SH3 domain containing protein 7; SH3 domain-containing protein 7; SH3P7; src homology 3 domain containing protein HIP 55;
抗原和靶标
- Q9UJU6 DBNL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAANLSRNGPALQEAYVRVVTEKSPTDWALFTYEGNSNDIRVAGTGEGGLEEMVEELNSGKVMYAFCRVKDPNSGLPKFVLINWTGEGVNDVRKGACASHVSTMASFLKGAHVTINARAEEDVEPECIMEKVAKASGANYSFHKESGRFQDVGPQAPVGSVYQKTNAVSEIKRVGKDSFWAKAEKEEENRRLEEKRRAEEAQRQLEQERRERELREAARREQRYQEQGGEASPQRTWEQQQEVVSRNRNEQESAVHPREIFKQKERAMSTTSISSPQPGKLRSPFLQKQLTQPETHFGREPAAAISRPRADLPAEEPAPSTPPCLVQAEEEAVYEEPPEQETFYEQPPLVQQQGAGSEHIDHHIQGQGLSGQGLCARALYDYQAADDTEISFDPENLITGIEVIDEGWWRGYGPDGHFGMFPANYVELIE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UJU6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| Y16 | Phosphorylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| T45 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| C127 | S-Nitrosylation | Uniprot | |
| K131 | Acetylation | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| K144 | Methylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| Y162 | Phosphorylation | Uniprot | |
| K164 | Methylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| T165 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| K172 | Methylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K176 | Acetylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| Y224 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| T270 | Phosphorylation | Uniprot | |
| T271 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S275 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K280 | Acetylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| K288 | Acetylation | Uniprot | |
| K288 | Methylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| T291 | Phosphorylation | Uniprot | |
| T295 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| T321 | Phosphorylation | Uniprot | |
| Y334 | Phosphorylation | P43403 (ZAP70) | Uniprot |
| Y344 | Phosphorylation | P43403 (ZAP70) | Uniprot |
研究背景
Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes (By similarity). May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes.
Degraded by caspases during apoptosis.
Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium. Cell projection>Ruffle. Cytoplasm>Cell cortex. Cytoplasm>Cytosol. Cell junction>Synapse. Perikaryon. Cell projection>Neuron projection. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle>Clathrin-coated vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Golgi apparatus membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Podosome. Early endosome. Cell projection>Dendrite. Cell junction>Synapse>Postsynaptic density.
Note: Associates with lamellipodial actin and membrane ruffles. Colocalizes with actin and cortactin at podosome dots and podosome rosettes.
Interacts with SHANK2, SHANK3 and SYN1. Interacts with FGD1 and DNM1. Interacts with ANKRD54. Interacts with COBL. Interacts with WASL and WIPF1 (By similarity). Interacts with MAP4K1 and PRAM1.
The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
Belongs to the ABP1 family.
限制条款
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