G6PC Antibody - #DF12610

Fig. 1. Celastrol reduces blood glucose levels in db/db mice, promotes insulin secretion, and improves hepatic glucose homeostasis metabolism. (A) The chemical structure of celastrol.(B–D) Effects of celastrol on blood glucose level (B), body weight (C), and food intake (D) in db/db mice. (E and F) Effect of celastrol on glucose tolerance test (GTT) results in db/db mice groups (E), area under the curve (AUC) for each group stated in (E) calculated using Graphpad Prism software v8.0.2 and plotted (F). All the data were expressed as mean ± SD (n = 6 – 7). §P < 0.05 or §§P < 0.01 vs. WT group. *P < 0.05 or **P < 0.01 vs. db/db group. (G) Effects of celastrol on insulin secretion in pancreas of db/db mice detected via immunohistochemical assay. (H) Effects of celastrol on insulin secretion in INS-1 cells. Student's t-test: §P < 0.05 vs. vehicle control group, **P < 0.01 vs. HG (high glucose) group. (I) INS-1 cells pretreated with 33.3 mM glucose (HG) for 2 h followed by celastrol (0.16, 0.5, or 1.5 μM) treatment for 24 h. Next, the cells were collected and subjected to western blot analysis. (J–K) HepG2 cells were pretreated with 33.3 mM glucose (HG) for 2 h followed by treatment with celastrol (0.1, 0.3, 1, or 3 μM) for 24 h. After treatment, the cells were incubated with 2-NBDG for 20 min, and these images were obtained using a fluorescence microscope (original magnification, 200 ×) (J). Quantitative 2NBDG uptake from each simple (K). Student's t-test:§§P < 0.01 vs. control group; **P < 0.01 vs. HG (high glucose) group. (L) HepG2 cells were pretreated with 33.3 mM glucose (HG) for 2 h and then treated with celastrol (1, 2, or 4 μM) for 24 h. The treated cells were collected and examined via western blotting.