| 产品: | IDH3A 抗体 |
| 货号: | DF12641 |
| 描述: | Rabbit polyclonal antibody to IDH3A |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus |
| 分子量: | 40 kDa; 40kD(Calculated). |
| 蛋白号: | P50213 |
| RRID: | AB_2845603 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12641, RRID:AB_2845603.
展开/折叠
H IDH alpha; HIDH alpha; IDH3A; IDH3A_HUMAN; Isocitrate dehydrogenase (NAD+) alpha chain precursor; Isocitrate dehydrogenase [NAD] subunit alpha; Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial; Isocitrate dehydrogenase 3 (NAD+) alpha; Isocitrate dehydrogenase; Isocitric dehydrogenase; Isocitric dehydrogenase subunit alpha; mitochondrial; NAD; NAD(+)-specific ICDH subunit alpha; NAD(H) specific isocitrate dehydrogenase alpha subunit precursor; NAD+ specific ICDH; NADsubunit alpha mitochondrial precursor; OTTHUMP00000184906;
抗原和靶标
- P50213 IDH3A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGPAWISKVSRLLGAFHNPKQVTRGFTGGVQTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P50213 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| R66 | Methylation | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| K77 | Acetylation | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| K90 | Acetylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| K177 | Acetylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| K214 | Acetylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| K343 | Acetylation | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| K350 | Ubiquitination | Uniprot |
研究背景
Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.
Mitochondrion.
Heterooligomer of subunits alpha (IDH3A), beta (IDH3B), and gamma (IDH3G) in the apparent ratio of 2:1:1. The heterodimer containing one IDH3A and one IDH3B subunit and the heterodimer containing one IDH3A and one IDH3G subunit assemble into a heterotetramer (which contains two subunits of IDH3A, one of IDH3B and one of IDH3G) and further into the heterooctamer.
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
研究领域
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
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