| 产品: | INVS 抗体 |
| 货号: | DF12644 |
| 描述: | Rabbit polyclonal antibody to INVS |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 97 kDa; 118kD(Calculated). |
| 蛋白号: | Q9Y283 |
| RRID: | AB_2845606 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12644, RRID:AB_2845606.
展开/折叠
INV; Inversion of embryo turning homolog; inversion of embryonic turning; INVS; Nephrocystin 2; Nephrocystin-2; Nephrocystin2; nephronophthisis 2 (infantile); NPH2; NPHP2;
抗原和靶标
- Q9Y283 INVS_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNKSENLLFAGSSLASQVHAAAVNGDKGALQRLIVGNSALKDKEDQFGRTPLMYCVLADRLDCADALLKAGADVNKTDHSQRTALHLAAQKGNYRFMKLLLTRRANWMQKDLEEMTPLHLTTRHRSPKCLALLLKFMAPGEVDTQDKNKQTALHWSAYYNNPEHVKLLIKHDSNIGIPDVEGKIPLHWAANHKDPSAVHTVRCILDAAPTESLLNWQDYEGRTPLHFAVADGNVTVVDVLTSYESCNITSYDNLFRTPLHWAALLGHAQIVHLLLERNKSGTIPSDSQGATPLHYAAQSNFAETVKVFLKHPSVKDDSDLEGRTSFMWAAGKGSDDVLRTMLSLKSDIDINMADKYGGTALHAAALSGHVSTVKLLLENNAQVDATDVMKHTPLFRACEMGHKDVIQTLIKGGARVDLVDQDGHSLLHWAALGGNADVCQILIENKINPNVQDYAGRTPLQCAAYGGYINCMAVLMENNADPNIQDKEGRTALHWSCNNGYLDAIKLLLDFAAFPNQMENNEERYTPLDYALLGERHEVIQFMLEHGALSIAAIQDIAAFKIQAVYKGYKVRKAFRDRKNLLMKHEQLRKDAAAKKREEENKRKEAEQQKGRRSPDSCRPQALPCLPSTQDVPSRQSRAPSKQPPAGNVAQGPEPRDSRGSPGGSLGGALQKEQHVSSDLQGTNSRRPNETAREHSKGQSACVHFRPNEGSDGSRHPGVPSVEKSRGETAGDERCAKGKGFVKQPSCIRVAGPDEKGEDSRRAAASLPPHDSHWKPSRRHDTEPKAKCAPQKRRTQELRGGRCSPAGSSRPGSARGEAVHAGQNPPHHRTPRNKVTQAKLTGGLYSHLPQSTEELRSGARRLETSTLSEDFQVSKETDPAPGPLSGQSVNIDLLPVELRLQIIQRERRRKELFRKKNKAAAVIQRAWRSYQLRKHLSHLRHMKQLGAGDVDRWRQESTALLLQVWRKELELKFPQTTAVSKAPKSPSKGTSGTKSTKHSVLKQIYGCSHEGKIHHPTRSVKASSVLRLNSVSNLQCIHLLENSGRSKNFSYNLQSATQPKNKTKP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y283 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K41 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| T200 | Phosphorylation | Uniprot | |
| K310 | Ubiquitination | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| K579 | Ubiquitination | Uniprot | |
| K584 | Ubiquitination | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| S658 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| R726 | Methylation | Uniprot | |
| R734 | Methylation | Uniprot | |
| S746 | Phosphorylation | Uniprot | |
| R799 | Methylation | Uniprot | |
| T864 | Phosphorylation | Uniprot | |
| S865 | Phosphorylation | Uniprot | |
| T866 | Phosphorylation | Uniprot | |
| S980 | Phosphorylation | Uniprot | |
| K984 | Acetylation | Uniprot | |
| S985 | Phosphorylation | Uniprot | |
| K988 | Acetylation | Uniprot | |
| K1012 | Ubiquitination | Uniprot | |
| S1019 | Phosphorylation | Uniprot | |
| S1030 | Phosphorylation | Uniprot | |
| S1032 | Phosphorylation | Uniprot | |
| K1062 | Acetylation | Uniprot |
研究背景
Required for normal renal development and establishment of left-right axis. Probably acts as a molecular switch between different Wnt signaling pathways. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. Involved in the organization of apical junctions in kidney cells together with NPHP1, NPHP4 and RPGRIP1L/NPHP8 (By similarity). Does not seem to be strictly required for ciliogenesis (By similarity).
May be ubiquitinated via its interaction with APC2.
Hydroxylated at Asn-75, most probably by HIF1AN.
Cytoplasm. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Spindle. Membrane>Peripheral membrane protein. Nucleus. Cell projection>Cilium.
Note: Associates with several components of the cytoskeleton including ciliary, random and polarized microtubules. During mitosis, it is recruited to mitotic spindle. Frequently membrane-associated, membrane localization is dependent upon cell-cell contacts and is redistributed when cell adhesion is disrupted after incubation of the cell monolayer with low-calcium/EGTA medium.
Widely expressed. Strongly expressed in the primary cilia of renal tubular cells.
Binds calmodulin via its IQ domains. Interacts with APC2. Interacts with alpha-, beta-, and gamma-catenin. Interacts with N-cadherin (CDH2). Interacts with microtubules (By similarity). Interacts with NPHP1. Interacts with DVL1, PRICKLE (PRICKLE1 or PRICKLE2) and Strabismus (VANGL1 or VANGL2). Interacts with IQCB1; the interaction likely requires additional interactors. Component of a complex containing at least ANKS6, INVS, NEK8 and NPHP3. ANKS6 may organize complex assembly by linking INVS and NPHP3 to NEK8 and INVS may target the complex to the proximal ciliary axoneme.
The D-box 1 (destruction box 1) mediates the interaction with APC2, and may act as a recognition signal for degradation via the ubiquitin-proteasome pathway.
研究领域
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
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