APP Antibody - #AF6084

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产品: APP 抗体
货号: AF6084
描述: Rabbit polyclonal antibody to APP
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Horse, Chicken, Xenopus
蛋白号: P05067
RRID: AB_2834853

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
APP Antibody detects endogenous levels of total APP.
RRID:
AB_2834853
引用格式: Affinity Biosciences Cat# AF6084, RRID:AB_2834853.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

A4_HUMAN; AAA; ABETA; ABPP; AD1; AICD-50; AICD-57; AICD-59; AID(50); AID(57); AID(59); Alzheimer disease amyloid protein; amyloid beta A4 protein; Amyloid intracellular domain 50; Amyloid intracellular domain 57; Amyloid intracellular domain 59; amyloid of aging and alzheimer disease; APP; APPI; beta-amyloid peptide; Beta-APP40; Beta-APP42; C31; Cerebral vascular amyloid peptide; CTFgamma; CVAP; Gamma-CTF(50); Gamma-CTF(57); Gamma-CTF(59); peptidase nexin-II; PN-II; PreA4; Protease nexin-II; S-APP-alpha; S-APP-beta;

抗原和靶标

免疫原:

A synthesized peptide derived from human APP, corresponding to a region within C-terminal amino acids.

基因/基因ID:
APP(351)
描述:
APP a cell surface receptor that influences neurite growth, neuronal adhesion and axonogenesis. Cleaved by secretases to form a number of peptides, some of which bind to the acetyltransferase complex Fe65/TIP60 to promote transcriptional activation.

研究领域

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Organismal Systems > Nervous system > Serotonergic synapse.

文献引用

1). NAD+-boosting agent nicotinamide mononucleotide potently improves mitochondria stress response in Alzheimer's disease via ATF4-dependent mitochondrial UPR. Cell death & disease, 2024 (PubMed: 39394148) [IF=8.1]
2). Cerebroprotein hydrolysate attenuates neurodegenerative changes in Alzheimer’s mice model via ferroptosis pathway. Frontiers in Pharmacology, 2023 [IF=5.6]
3). 17β-Trenbolone increases the release of lipocalin 2 via the brain‒liver axis and causes Alzheimer's disease-like symptoms in CSDS-induced mice. MOLECULAR NEUROBIOLOGY, 2025 [IF=5.1]
4). Tetrahydroxy stilbene glycoside ameliorates Alzheimer's disease in APP/PS1 mice via glutathione peroxidase related ferroptosis. International Immunopharmacology, 2021 (PubMed: 34333354) [IF=4.8]

Application: IF/ICC    Species: Mouse    Sample: brain

Fig. 8. TSG regulates the ferroptosis in various glial cells in APP/PS1 and WT mouse brains. Western blotting assay was performed to detect the expression of related proteins. (A) The protein expression levels of GPX4, GPX1, FTH1, xCT, CD98, DMT1, ACSL4, NCOA4, Ferritin Light Chain and Ferritin Heavy Chain were assessed. The levels were normalized to β-actin. (B) GPX4, GFAP and DAPI fluorescence staining co-localization of WT, APP/PS1 and TSG 60 mg/kg group. (C) GPX4, Iba1 and DAPI fluorescence staining co-localization of WT, APP/PS1 and TSG 60 mg/kg group. P# < 0.05, P## < 0.01, P### < 0.001 vs WT group. P* < 0.05, P** < 0.01, P*** < 0.001 vs APP/PS1 group. 2×, 100 × magnification, scare bar: 500 and 100 μm, n = 5/group.
5). PLC-CN-NFAT1 signaling-mediated Aβ and IL-1β crosstalk synergistically promotes hippocampal neuronal damage. International immunopharmacology, 2024 (PubMed: 38749336) [IF=4.8]
6). Carnitine palmitoyltransferase 1 (CPT1) alleviates oxidative stress and apoptosis of hippocampal neuron in response to beta-Amyloid peptide fragment Aβ25-35. Bioengineered, 2021 (PubMed: 34424821) [IF=4.2]

Application: WB    Species: Mice    Sample: Aβ25-35-induced HT22 cells

Figure 5. CPT1C overexpression decreased the deposition of AD marker proteins in Aβ25-35-induced HT22 cells. Following transfection of Ov-CPT1C or Ov-NC for 24 h, HT22 cells were treated with Aβ25–35 for another 24 h (a) The mRNA expressions of App, p-Tau and Bace-1 were evaluated using RT-qPCR. (b) The protein expressions of App, p-Tau and Bace-1 were evaluated using western blot. ***P < 0.001 vs. Control group, ###P < 0.001 vs Aβ25-35 + Ov-NC.
7). LongShengZhi Capsule attenuates Alzheimer-like pathology in APP/PS1 double transgenic mice by reducing neuronal oxidative stress and inflammation. Frontiers in Aging Neuroscience, 2020 (PubMed: 33328962) [IF=4.1]
8). Long-Term Administration of Triterpenoids From Ganoderma lucidum Mitigates Age-Associated Brain Physiological Decline via Regulating Sphingolipid Metabolism and Enhancing Autophagy in Mice. Frontiers in Aging Neuroscience, 2021 (PubMed: 34025387) [IF=4.1]

Application: WB    Species: mouse    Sample: brain

FIGURE 9 | Ameliorate effects of Ganodenic acid A by regulating sphingolipid metabolism in 3 × Tg-AD mice. (A) Experimental procedure in 3 × Tg-AD mice;(B) The AD biomarkers of p-Tau, Aβ, APOE, TREM2, CD33, the inflammatory cytokines of TNF-α and NF-κB p65 and the autophagy level of LC3A/B were measured in brain tissues
9). CENPE is a diagnostic and prognostic biomarker for cervical cancer. Heliyon, 2024 (PubMed: 39759304) [IF=4.0]
10). Polarization of Microglia to the M2 Phenotype in a Peroxisome Proliferator-Activated Receptor Gamma–Dependent Manner Attenuates Axonal Injury Induced by Traumatic Brain Injury in Mice. Journal of Neurotrauma, 2018 (PubMed: 29649924) [IF=3.9]

Application: IHC    Species: mouse    Sample: cerebral cortex

Fig. 2:| Immunohistochemical staining for beta-amyloid precursor protein (β-APP) in cerebral cortex after TBI in mice (A). Scale bar = 20 μm. Immunoreactivity for β-APP determined by average optical density (AOD), was attenuated by rosiglitazone and enhanced by GW992 (B). * p < 0.05, ** p < 0.01.
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