| 产品: | Nurr1/NR4A2 抗体 |
| 货号: | DF12678 |
| 描述: | Rabbit polyclonal antibody to Nurr1/NR4A2 |
| 应用: | WB |
| 反应: | Human |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 66 kDa; 67kD(Calculated). |
| 蛋白号: | P43354 |
| RRID: | AB_2845640 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12678, RRID:AB_2845640.
展开/折叠
HZF 3; HZF3; Immediate-early response protein NOT; Intermediate early receptor protein; NGFI B/nur77 beta type transcription factor homolog; NOT; Nr4a2; NR4A2_HUMAN; nuclear receptor of T cells; nuclear receptor related 1; Nuclear receptor subfamily 4 group A member 2; Nur related protein 1 homolog; nur related protein-1, human homolog of; Nurr 1; Orphan nuclear receptor NR4A2; Orphan nuclear receptor NURR1; RNR 1; RNR1; T cell nuclear receptor NOT; T-cell nuclear receptor NOT; TINUR; Transcriptionally inducible nuclear receptor; Transcriptionally inducible nuclear receptor related; Transcriptionally inducible nuclear receptor related 1; Transcriptionally-inducible nuclear receptor;
抗原和靶标
Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.
- P43354 NR4A2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPCVQAQYGSSPQGASPASQSYSYHSSGEYSSDFLTPEFVKFSMDLTNTEITATTSLPSFSTFMDNYSTGYDVKPPCLYQMPLSGQQSSIKVEDIQMHNYQQHSHLPPQSEEMMPHSGSVYYKPSSPPTPTTPGFQVQHSPMWDDPGSLHNFHQNYVATTHMIEQRKTPVSRLSLFSFKQSPPGTPVSSCQMRFDGPLHVPMNPEPAGSHHVVDGQTFAVPNPIRKPASMGFPGLQIGHASQLLDTQVPSPPSRGSPSNEGLCAVCGDNAACQHYGVRTCEGCKGFFKRTVQKNAKYVCLANKNCPVDKRRRNRCQYCRFQKCLAVGMVKEVVRTDSLKGRRGRLPSKPKSPQEPSPPSPPVSLISALVRAHVDSNPAMTSLDYSRFQANPDYQMSGDDTQHIQQFYDLLTGSMEIIRGWAEKIPGFADLPKADQDLLFESAFLELFVLRLAYRSNPVEGKLIFCNGVVLHRLQCVRGFGEWIDSIVEFSSNLQNMNIDISAFSCIAALAMVTERHGLKEPKRVEELQNKIVNCLKDHVTFNNGGLNRPNYLSKLLGKLPELRTLCTQGLQRIFYLKLEDLVPPPAIIDKLFLDTLPF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P43354 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y71 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| T129 | Phosphorylation | Uniprot | |
| T132 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S177 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| T185 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| Y275 | Phosphorylation | Uniprot | |
| T279 | Phosphorylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| T335 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S347 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| K530 | Ubiquitination | Uniprot | |
| K558 | Sumoylation | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| K577 | Sumoylation | Uniprot |
研究背景
Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons (By similarity).
Cytoplasm. Nucleus.
Note: Mostly nuclear; oxidative stress promotes cytoplasmic localization.
Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.
Interacts with SFPQ, NCOR2, SIN3A and HADC1. The interaction with NCOR2 increases in the absence of PITX3. Interacts with PER2 (By similarity).
the ligand-binding domain (LBD) contains no cavity as a result of the tight packing of side chains from several bulky hydrophobic residues in the region normally occupied by ligands. NR4A2 lacks a 'classical' binding site for coactivators (PubMed:12774125).
Belongs to the nuclear hormone receptor family. NR4 subfamily.
研究领域
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
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