eIF2 alpha Antibody - #AF6087

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产品: eIF2 alpha 抗体
货号: AF6087
描述: Rabbit polyclonal antibody to eIF2 alpha
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat, Pig
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 38kDa; 36kD(Calculated).
蛋白号: P05198
RRID: AB_2834856

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Pig
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
eIF2 alpha Antibody detects endogenous levels of total eIF2 alpha.
RRID:
AB_2834856
引用格式: Affinity Biosciences Cat# AF6087, RRID:AB_2834856.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EIF 2 alpha; EIF 2; EIF 2A; EIF 2alpha; eIF-2-alpha; eIF-2A; EIF-2alpha; EIF2 alpha; EIF2; EIF2A; EIF2S1; Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa; Eukaryotic translation initiation factor 2 subunit 1 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; IF2A_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P05198(IF2A_HUMAN) >>Visit HPA database.

基因/基因ID:
EIF2S1(1965)
描述:
eIF2A a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex.
序列:
MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEVDGDDDAEEMEAKAED

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P05198 作为底物

Site PTM Type Enzyme
S5 Phosphorylation
K12 Ubiquitination
S26 Phosphorylation
S49 Phosphorylation Q9NZJ5 (EIF2AK3) , Q9BQI3 (EIF2AK1) , P19525 (EIF2AK2) , Q75MR0 (HRI)
S52 Phosphorylation B4DS64 (PRKRIR) , P51812 (RPS6KA3) , P19525 (EIF2AK2) , P28482 (MAPK1) , Q75MR0 (HRI) , Q9BQI3 (EIF2AK1) , Q9NZJ5 (EIF2AK3) , Q05655 (PRKCD) , Q16539 (MAPK14) , Q9P2K8 (EIF2AK4)
S58 Phosphorylation
K61 Ubiquitination
K80 Ubiquitination
Y82 Phosphorylation
S86 Phosphorylation
K87 Ubiquitination
S91 Phosphorylation
K97 Ubiquitination
K101 Acetylation
K101 Ubiquitination
K104 Ubiquitination
K106 Ubiquitination
S110 Phosphorylation
K123 Ubiquitination
R133 Methylation
K141 Acetylation
K141 Ubiquitination
K143 Acetylation
K143 Ubiquitination
Y147 Phosphorylation
Y150 Phosphorylation
K154 Methylation
K154 Ubiquitination
S158 Phosphorylation
T185 Phosphorylation
K190 Ubiquitination
Y200 Phosphorylation
Y202 Phosphorylation
K209 Ubiquitination
S219 Phosphorylation
K226 Ubiquitination
Y235 Phosphorylation
T245 Phosphorylation
K259 Ubiquitination
K265 Ubiquitination
R266 Methylation
K276 Ubiquitination
T279 Phosphorylation
T281 Phosphorylation
T284 Phosphorylation
K312 Ubiquitination

研究背景

功能:

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

翻译修饰:

Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation (By similarity).

细胞定位:

Cytoplasm>Stress granule.
Note: Colocalizes with NANOS3 in the stress granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interaction with METAP2 protects EIF2S1 from inhibitory phosphorylation (By similarity). Interacts with ABCF1 isoform 2. Associates with ribosomes. Interacts with DDX3X in an RNA-independent manner.

蛋白家族:

Belongs to the eIF-2-alpha family.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

文献引用

1). Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. NUCLEIC ACIDS RESEARCH, 2020 (PubMed: 32710621) [IF=14.9]

Application: WB    Species: mouse    Sample: liver

Figure 7. Effect of A22 on ameliorating apoptosis, ER stress, inflammation, metabolic syndrome, and fibrogenesis in HF diet-fed mice. (A) Effect of A22 on BCL-2 gene transcription. (B) Effect of A22 on BAX gene transcription. (C) Effect of A22 on expressions of apoptosis-related proteins in liver. The extracted proteins from the liver were immunoblotted with specific antibodies, and quantified based on the loading control of ACTIN. (D) Effect of A22 on ER stress. The UPR proteins (IRE-1, PERK, elF-2 and CHOP) were analyzed by using western Blot. (E) Effect of A22 on expressions of inflammatory factors. (F) Effect of A22 on expressions of fibrogenic proteins.
2). Betulinic acid chemosensitizes breast cancer by triggering ER stress-mediated apoptosis by directly targeting GRP78. Cell Death & Disease, 2018 (PubMed: 29802332) [IF=9.0]

Application: WB    Species: human    Sample: MCF-7,MDA-MB-231

Fig. 5 BA triggers breast cancer cells apoptosis via ER stress-mediated pathway. a MCF-7 and MDA-MB-231 cells were treated with the indicated concentrations of BA for 24 h, and the protein levels of ER stress-associated signals were stimulated by BA in a dose-dependent manner, including GRP78, p-PERK/PERK, p-eIF2α/eIF2α, CHOP, and caspase-12. b MCF-7 and MDA-MB-231 cells were treated with BA alone or in combination with taxol for 24 h, the expression levels of GRP78, p-PERK/PERK, p-eIF2α/eIF2α, CHOP, and caspase-12 were also significantly upregulated following drug administration, especially in the co-treatment group, indicating the ER stress-mediated apoptosis pathway was aggravatedly activated by drug combination.
3). Cyanidin-3-O-glucoside and protocatechuic acid alleviate heat stress-induced testicular damage. Food & Function, 2023 (PubMed: 36756975) [IF=6.1]
4). -bambuterol and its enantiomers: Potential improvement of (R)-bambuterol in mice with colitis. International Immunopharmacology, 2022 (PubMed: 34974400) [IF=5.6]
5). Protective Effect of Patchouli Alcohol Against High-Fat Diet Induced Hepatic Steatosis by Alleviating Endoplasmic Reticulum Stress and Regulating VLDL Metabolism in Rats. Frontiers in Pharmacology, 2019 (PubMed: 31632274) [IF=5.6]

Application: WB    Species: rat    Sample: liver

FIGURE 5 | PA treatment attenuated HFD-induced VLDLR expression in rats. (A) Representative immunoreactive bands of eIF2α, p-eIF2α, ATF4, and VLDLR
6). Inhibition of High-Temperature Requirement Protein A2 Protease Activity Represses Myogenic Differentiation via UPRmt. International journal of molecular sciences, 2022 (PubMed: 36233059) [IF=5.6]

Application: WB    Species: Mouse    Sample:

Figure 7 HtrA2/Omi protease deficiency activates UPRmt signaling during myogenic differentiation. (a) Western blot of IMS proteases OMA1, YME1L1, and HtrA2 on D1, D3, and D5 treated with UCF101 (20 μM) or untreated and quantification of western blots relative to GM (n = 3). (b,c) Western blot of protein expression and mRNA transcript levels of the UPRmt upstream signals CHOP, eIF2α (and its phosphorylation), related molecular chaperones HSP10, 60, and 75, and matrix protease LONP1 at D1, D3, and D5 in C2C12 myoblasts treated with UCF101 (20 μM) or untreated, and quantification of WB assays of CHOP and p-eIF2α relative to GM (n = 3). LONP1, lon peptidase 1; CHOP (Ddit3), DNA-damage-inducible transcript 3; HSP, heat shock protein; eIF2α, eukaryotic translation initiation factor 2 subunit alpha; UPRmt, mitochondrial unfold protein response; Clpx, caseinolytic mitochondrial matrix peptidase chaperone subunit X; Clpp, aseinolytic mitochondrial matrix peptidase proteolytic subunit. Student’s t-tests were used. Statistical significance: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001.
7). CTRP1 attenuates cerebral ischemia/reperfusion injury via the PERK signaling pathway. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 34422821) [IF=5.5]

Application: WB    Species: Rat    Sample: cortex

FIGURE 7 The function of CTRP1 on apoptosis and PERK signal pathway was inhibited by CCT020312. (A) Apoptosis was analyzed by TUNEL, n = 3 per group. The representative images were acquired under × 200 magnification, scale bars = 100 μm. (B) Western blot analyzed the expression CTRP1, BAX, CHOP, GRP78, ATF4, p-PERK, PERK, p-eIF2α, and eIF2α in the cortex. n = 6 per group. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05 vs. sham group, ####p < 0.0001, ##p < 0.01, #p < 0.05 vs. MCAO/R + LV-NC group, @@p < 0.01, @p < 0.05 vs. MCAO/R + LV-CTRP1 group.
8). ATP citrate lyase inhibitor triggers endoplasmic reticulum stress to induce hepatocellular carcinoma cell apoptosis via p‐eIF2α/ATF4/CHOP axis. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021 (PubMed: 33393219) [IF=5.3]

Application: WB    Species: Human    Sample: HepG2 cells

FIGURE 5 ACLY inhibitor triggers ER stress and activates p‐eIF2α/ATF4/CHOP axis in vitro. Western blot analysis of (A) ER stress‐related proteins (p‐eIF2α, eIF2α, ATF4 and CHOP) and (B) UPR signal transduction molecules (p‐PERK, PERK, p‐IRE1α, IRE1α and sXBP1) in HepG2 cells after administration of BMS‐303141. ATF4p‐eIF2α, eIF2α were activated 3 h post‐treatment; CHOP was activated 8 h post‐treatment. (* P < .05, ** P < .01 and *** P < .001, compared with control group) (C) Western blot analysis of protein expression after ATF4 knockdown. (D) Annexin V‐FITC/PI double staining was performed to determine the apoptosis rate of HepG2 cells after ATF4 knockdown via flow cytometry. (* P < .05, ** P < .01 and *** P < .001, compared with con siRNA group). All experiments were repeated 3 times
9). MiR-185-5p ameliorates endoplasmic reticulum stress and renal fibrosis by downregulation of ATF6. LABORATORY INVESTIGATION, 2020 (PubMed: 32514126) [IF=5.0]

Application: WB    Species: human    Sample: HK2 cells

Supplementary figure 2. |miR-185-5p suppresses the activation of the IRE1/XBP1 and PERK/eIF2α/ATF4 branches in TGF-β1-induced HK2 cells.(C, D) Western blot analysis of p-PERK, PERK, p-eIF2α, eIF2α, and ATF4 proteins.Data are presented as the mean ± SD (n = 3) calculated from one-way ANOVA with Tukey’s test.
10). Protective autophagy alleviates neurotoxin-gelsenicine induced apoptosis through PERK signaling pathway in Neuro-2a cells. TOXICOLOGY, 2022 (PubMed: 35588915) [IF=4.5]
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