Ribophorin I Antibody | Affinity Biosciences LTD|亲科生物官网

WB
IF/ICC

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Western blot analysis of extracts from K-562 cells, using RPN1 Antibody.

Western blot analysis of extracts from COS-7, using RPN1 Antibody.

产品:	Ribophorin I 抗体
货号:	DF12727
描述:	Rabbit polyclonal antibody to Ribophorin I
应用:	WB IF/ICC
反应:	Human, Mouse, Rat, Monkey
预测:	Pig, Bovine, Horse, Rabbit, Dog, Xenopus
分子量:	69 kDa; 69kD(Calculated).
蛋白号:	P04843
RRID:	AB_2845688

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat,Monkey

预测:

Pig(92%), Bovine(92%), Horse(92%), Rabbit(92%), Dog(92%), Xenopus(83%)

克隆:

Polyclonal

特异性:

Ribophorin I Antibody detects endogenous levels of total Ribophorin I.

RRID:

AB_2845688
引用格式: Affinity Biosciences Cat# DF12727, RRID:AB_2845688.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

D6Wsu137e; Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit; Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; EC 2.4.1.119; Oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); OST1; RBPH1; Ribophorin I; Ribophorin-1; RPN-I; Rpn1; RPN1_HUMAN;

抗原和靶标

免疫原:

Uniprot:

P04843(RPN1_HUMAN) >>Visit HPA database.

基因/基因ID:

RPN1(6184)

表达:

P04843 RPN1_HUMAN:

Expressed in all tissues tested.

序列:

P04843 RPN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEAPAAGLFLLLLLGTWAPAPGSASSEAPPLINEDVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFLLALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLHPYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYSQDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSFKTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFPLFGGWKTHYIVGYNLPSYEYLYNLGDQYALKMRFVDHVFDEQVIDSLTVKIILPEGAKNIEIDSPYEISRAPDELHYTYLDTFGRPVIVAYKKNLVEQHIQDIVVHYTFNKVLMLQEPLLVVAAFYILFFTVIIYVRLDFSITKDPAAEARMKVACITEQVLTLVNKRIGLYRHFDETVNRYKQSRDISTLNSGKKSLETEHKALTSEIALLQSRLKTEGSDLCDRVSEMQKLDAQVKELVLKSAVEAERLVAGKLKKDTYIENEKLISGKRQELVTKIDHILDAL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

Horse

Bovine

Dog

Rabbit

Xenopus

Zebrafish

Sheep

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P04843 作为底物

P04843

Site	PTM Type	Source
K37	Ubiquitination	Uniprot
T39	Phosphorylation	Uniprot
K48	Ubiquitination	Uniprot
K89	Ubiquitination	Uniprot
K116	Ubiquitination	Uniprot
Y135	Phosphorylation	Uniprot
T136	Phosphorylation	Uniprot
Y161	Phosphorylation	Uniprot
S164	Phosphorylation	Uniprot
K187	Acetylation	Uniprot
K187	Ubiquitination	Uniprot
S194	Phosphorylation	Uniprot
Y200	Phosphorylation	Uniprot
Y209	Phosphorylation	Uniprot
K215	Ubiquitination	Uniprot
S222	Phosphorylation	Uniprot
K258	Ubiquitination	Uniprot
Y264	Phosphorylation	Uniprot
Y266	Phosphorylation	Uniprot
K281	Ubiquitination	Uniprot
Y292	Phosphorylation	Uniprot
Y293	Phosphorylation	Uniprot
N299	N-Glycosylation	Uniprot
K352	Ubiquitination	Uniprot
K371	Ubiquitination	Uniprot
K379	Ubiquitination	Uniprot
S385	Phosphorylation	Uniprot
Y387	Phosphorylation	Uniprot
S390	Phosphorylation	Uniprot
Y398	Phosphorylation	Uniprot
T399	Phosphorylation	Uniprot
K413	Acetylation	Uniprot
K413	Ubiquitination	Uniprot
K414	Acetylation	Uniprot
K414	Ubiquitination	Uniprot
K465	Ubiquitination	Uniprot
K474	Ubiquitination	Uniprot
C477	S-Nitrosylation	Uniprot
K488	Ubiquitination	Uniprot
S510	Phosphorylation	Uniprot
S514	Phosphorylation	Uniprot
K516	Acetylation	Uniprot
K516	Ubiquitination	Uniprot
K517	Ubiquitination	Uniprot
S518	Phosphorylation	Uniprot
K524	Acetylation	Uniprot
K524	Ubiquitination	Uniprot
S528	Phosphorylation	Uniprot
S535	Phosphorylation	Uniprot
K538	Acetylation	Uniprot
K538	Sumoylation	Uniprot
K538	Ubiquitination	Uniprot
S542	Phosphorylation	Uniprot
R547	Methylation	Uniprot
K553	Acetylation	Uniprot
K553	Ubiquitination	Uniprot
K559	Ubiquitination	Uniprot
K564	Ubiquitination	Uniprot
K576	Ubiquitination	Uniprot
K578	Ubiquitination	Uniprot
K579	Ubiquitination	Uniprot
K587	Acetylation	Uniprot
K587	Ubiquitination	Uniprot
T598	Phosphorylation	Uniprot
K599	Ubiquitination	Uniprot

研究背景

P04843_RPN1_HUMAN

功能:

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

细胞定位:

Endoplasmic reticulum. Endoplasmic reticulum membrane>Single-pass type I membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

组织特异性:

Expressed in all tissues tested.

亚基结构:

Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).

蛋白家族:

Belongs to the OST1 family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

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Ribophorin I Antibody - #DF12727

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