(1)
(4)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12752, RRID:AB_2845713.
展开/折叠
HSAN; HSAN1; HSN1; LBC1; LCB 1; LCB1; Long chain base biosynthesis protein 1; MGC14645; Serine C palmitoyltransferase; Serine palmitoyl CoA transferase 1; Serine palmitoyltransferase 1; Serine palmitoyltransferase long chain base subunit 1; Serine palmitoyltransferase subunit 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1; SPTC1_HUMAN; SPTI; SPTLC 1; SPTLC1;
抗原和靶标
- O15269 SPTC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATATEQWVLVEMVQALYEAPAYHLILEGILILWIIRLLFSKTYKLQERSDLTVKEKEELIEEWQPEPLVPPVPKDHPALNYNIVSGPPSHKTVVNGKECINFASFNFLGLLDNPRVKAAALASLKKYGVGTCGPRGFYGTFDVHLDLEDRLAKFMKTEEAIIYSYGFATIASAIPAYSKRGDIVFVDRAACFAIQKGLQASRSDIKLFKHNDMADLERLLKEQEIEDQKNPRKARVTRRFIVVEGLYMNTGTICPLPELVKLKYKYKARIFLEESLSFGVLGEHGRGVTEHYGINIDDIDLISANMENALASIGGFCCGRSFVIDHQRLSGQGYCFSASLPPLLAAAAIEALNIMEENPGIFAVLKEKCGQIHKALQGISGLKVVGESLSPAFHLQLEESTGSREQDVRLLQEIVDQCMNRSIALTQARYLEKEEKCLPPPSIRVVVTVEQTEEELERAASTIKEVAQAVLL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O15269 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K45 | Ubiquitination | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| T53 | Phosphorylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| Y164 | Phosphorylation | P00519 (ABL1) | Uniprot |
| K197 | Ubiquitination | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K210 | Methylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| K434 | Acetylation | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| C438 | S-Nitrosylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| K465 | Ubiquitination | Uniprot |
研究背景
Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Required for adipocyte cell viability and metabolic homeostasis (By similarity).
Phosphorylation at Tyr-164 inhibits activity and promotes cell survival.
Endoplasmic reticulum membrane>Single-pass membrane protein.
Widely expressed. Not detected in small intestine.
Heterodimer with SPTLC2 or SPTLC3. Component of the serine palmitoyltransferase (SPT) complex, composed of SPTLC1, either SPTLC2 or SPTLC3, and either SPTSSA or SPTSSB. The composition of the complex will define the substrate specificity. Interacts with SPTSSA and SPTSSB; the interaction is direct. Interacts with ORMDL3. Interacts with RTN4 (isoform B) (By similarity).
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
研究领域
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Metabolism > Lipid metabolism > Sphingolipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
文献引用
Application: WB Species: Mice Sample: H9C2 cells
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