| 产品: | AKAP8L 抗体 |
| 货号: | DF12818 |
| 描述: | Rabbit polyclonal antibody to AKAP8L |
| 应用: | WB |
| 反应: | Human |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 72 kDa; 72kD(Calculated). |
| 蛋白号: | Q9ULX6 |
| RRID: | AB_2845779 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12818, RRID:AB_2845779.
展开/折叠
A kinase (PRKA) anchor protein 8 like; A kinase anchor protein 8 like; AKAP 8L; AKAP8 like protein; DKFZp434L0650; HA95; HAP95; Helicase A binding protein 95; Homologous to AKAP95 protein; NAKAP; NAKAP95; Neighbor of A kinase anchoring protein 95; Neighbor of AKAP95;
抗原和靶标
- Q9ULX6 AKP8L_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSYTGFVQGSETTLQSTYSDTSAQPTCDYGYGTWNSGTNRGYEGYGYGYGYGQDNTTNYGYGMATSHSWEMPSSDTNANTSASGSASADSVLSRINQRLDMVPHLETDMMQGGVYGSGGERYDSYESCDSRAVLSERDLYRSGYDYSELDPEMEMAYEGQYDAYRDQFRMRGNDTFGPRAQGWARDARSGRPMASGYGRMWEDPMGARGQCMSGASRLPSLFSQNIIPEYGMFQGMRGGGAFPGGSRFGFGFGNGMKQMRRTWKTWTTADFRTKKKKRKQGGSPDEPDSKATRTDCSDNSDSDNDEGTEGEATEGLEGTEAVEKGSRVDGEDEEGKEDGREEGKEDPEKGALTTQDENGQTKRKLQAGKKSQDKQKKRQRDRMVERIQFVCSLCKYRTFYEDEMASHLDSKFHKEHFKYVGTKLPKQTADFLQEYVTNKTKKTEELRKTVEDLDGLIQQIYRDQDLTQEIAMEHFVKKVEAAHCAACDLFIPMQFGIIQKHLKTMDHNRNRRLMMEQSKKSSLMVARSILNNKLISKKLERYLKGENPFTDSPEEEKEQEEAEGGALDEGAQGEAAGISEGAEGVPAQPPVPPEPAPGAVSPPPPPPPEEEEEGAVPLLGGALQRQIRGIPGLDVEDDEEGGGGAP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9ULX6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y29 | Phosphorylation | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| Y125 | Phosphorylation | Uniprot | |
| C128 | S-Nitrosylation | Uniprot | |
| R131 | Methylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| R171 | Methylation | Uniprot | |
| R179 | Methylation | Uniprot | |
| R185 | Methylation | Uniprot | |
| R188 | Methylation | Uniprot | |
| R191 | Methylation | Uniprot | |
| Y197 | Phosphorylation | Uniprot | |
| R199 | Methylation | Uniprot | |
| R208 | Methylation | Uniprot | |
| R217 | Methylation | Uniprot | |
| R237 | Methylation | Uniprot | |
| R247 | Methylation | Uniprot | |
| K257 | Acetylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| T267 | Phosphorylation | Uniprot | |
| T268 | Phosphorylation | Uniprot | |
| R272 | Methylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| K290 | Ubiquitination | Uniprot | |
| T292 | Phosphorylation | Uniprot | |
| T294 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| T313 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| R397 | Methylation | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| K423 | Ubiquitination | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| Y435 | Phosphorylation | Uniprot | |
| K439 | Ubiquitination | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| K477 | Ubiquitination | Uniprot | |
| K500 | Acetylation | Uniprot | |
| K503 | Ubiquitination | Uniprot | |
| T504 | Phosphorylation | Uniprot | |
| K520 | Acetylation | Uniprot | |
| K533 | Ubiquitination | Uniprot | |
| K544 | Sumoylation | Uniprot | |
| K544 | Ubiquitination | Uniprot | |
| T550 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| S601 | Phosphorylation | Uniprot |
研究背景
Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export. Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis. May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta. Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8. May be involved in regulation of pre-mRNA splicing.
(Microbial infection) In case of EBV infection, may target PRKACA to EBNA-LP-containing nuclear sites to modulate transcription from specific promoters.
(Microbial infection) Can synergize with DHX9 to activate the CTE-mediated gene expression of type D retroviruses.
(Microbial infection) In case of HIV-1 infection, involved in the DHX9-promoted annealing of host tRNA(Lys3) to viral genomic RNA as a primer in reverse transcription; in vitro negatively regulates DHX9 annealing activity.
Phosphorylated on serine or threonine residues possibly by PKA; probably modulating the interaction with TMPO isoform Beta.
Nucleus. Nucleus matrix. Nucleus speckle. Nucleus>PML body. Cytoplasm.
Note: Colocalizes with PRPF40A in the nuclear matrix (PubMed:16391387). Nuclear at steady state but shuttles between the nucleus and cytoplasm (PubMed:10748171). The shuttling property has been questioned (PubMed:11034899). Colocalizes with EBNA-LP in PML bodies (PubMed:11884601).
Ubiquitously expressed. Expressed in the brain cortex (at protein level).
Interacts (via N-terminus) with DHX9 (via RGG region). Interacts with TMPO isoform Beta, PRPF40A, RNF43, lamin-B. Interacts with HDAC3; increased during mitosis. Interacts with EBV EBNA-LP. Interacts with HIV-1 reverse transcriptase/ribonuclease H.
Belongs to the AKAP95 family.
研究领域
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
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