ARD1A Antibody - #DF12828

Catalytic subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity. Acetylates amino termini that are devoid of initiator methionine. The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development. Acetylates HSPA1A and HSPA1B at 'Lys-77' which enhances its chaperone activity and leads to preferential binding to co-chaperone HOPX. Acetylates HIST1H4A. Acts as a negative regulator of sister chromatid cohesion during mitosis.

Cleaved by caspases during apoptosis.

Phosphorylation by IKBKB/IKKB at Ser-209 promotes its proteasome-mediated degradation.

Autoacetylated at Lys-136 which stimulates its catalytic activity.

Cytoplasm. Nucleus.
Note: Also present in the free cytosolic and cytoskeleton-bound polysomes.

Ubiquitous.

Component of the N-terminal acetyltransferase A (NatA) complex composed of NAA10 and NAA15 or NAA16. Interacts with HIF1A (via its ODD domain); the interaction increases HIF1A protein stability during normoxia, an down-regulates it when induced by hypoxia. Interacts with NAA50 and with the ribosome. Binds to MYLK. Interacts with NAA15. Associates with HYPK when in complex with NAA15. Interacts with NAA16. Interacts (via its C-terminal domain) with TSC2, leading to its acetylation. Interacts with IKBKB. Interacts with HSPA1A and HSPA1B leading to its acetylation.

Belongs to the acetyltransferase family. ARD1 subfamily.