| 产品: | ERO1L 抗体 |
| 货号: | DF12984 |
| 描述: | Rabbit polyclonal antibody to ERO1L |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 54 kDa; 54kD(Calculated). |
| 蛋白号: | Q96HE7 |
| RRID: | AB_2845945 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12984, RRID:AB_2845945.
展开/折叠
Endoplasmic oxidoreductin 1 like protein; Endoplasmic oxidoreductin-1-like protein; Endoplasmic reticulum oxidoreductase 1 alpha; Endoplasmic reticulum oxidoreductin 1-like; ERO1 alpha; ERO1 L; ERO1 Lalpha; ERO1 like protein alpha; ERO1-alpha; ERO1-L; ERO1-L-alpha; ERO1-like (S. cerevisiae); ERO1-like alpha; ERO1-like protein alpha; ERO1-like, S. cerevisiae, homolog of, alpha; ERO1A; ERO1A_HUMAN; ERO1L; ERO1LA; Oxidoreductin 1 Lalpha; Oxidoreductin-1-L-alpha; PRO865; UNQ434;
抗原和靶标
Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
- Q96HE7 ERO1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGRGWGFLFGLLGAVWLLSSGHGEEQPPETAAQRCFCQVSGYLDDCTCDVETIDRFNNYRLFPRLQKLLESDYFRYYKVNLKRPCPFWNDISQCGRRDCAVKPCQSDEVPDGIKSASYKYSEEANNLIEECEQAERLGAVDESLSEETQKAVLQWTKHDDSSDNFCEADDIQSPEAEYVDLLLNPERYTGYKGPDAWKIWNVIYEENCFKPQTIKRPLNPLASGQGTSEENTFYSWLEGLCVEKRAFYRLISGLHASINVHLSARYLLQETWLEKKWGHNITEFQQRFDGILTEGEGPRRLKNLYFLYLIELRALSKVLPFFERPDFQLFTGNKIQDEENKMLLLEILHEIKSFPLHFDENSFFAGDKKEAHKLKEDFRLHFRNISRIMDCVGCFKCRLWGKLQTQGLGTALKILFSEKLIANMPESGPSYEFHLTRQEIVSLFNAFGRISTSVKELENFRNLLQNIH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96HE7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y59 | Phosphorylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| R75 | Methylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| C131 | S-Nitrosylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| Y248 | Phosphorylation | Uniprot | |
| Y266 | Phosphorylation | Uniprot | |
| N280 | N-Glycosylation | Uniprot | |
| T293 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| T405 | Phosphorylation | Uniprot | |
| Y431 | Phosphorylation | Uniprot |
研究背景
Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1.
N-glycosylated.
The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397 (By similarity). The regulatory Cys-99/Cys-104 disulfide bond stabilizes the other regulatory bond Cys-94/Cys-131.
Endoplasmic reticulum membrane>Peripheral membrane protein>Lumenal side.
Note: The association with ERP44 is essential for its retention in the endoplasmic reticulum.
Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
Predominantly monomer. May function both as a monomer and a homodimer. Interacts with PDILT.
Belongs to the EROs family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.
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