产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13027, RRID:AB_2845988.
展开/折叠
CDC20 like 1b; CDC20 like protein 1; CDC20-like protein 1; CDC20C; CDH1; Cdh1/Hct1 homolog; Fizzy related protein 1; Fizzy related protein; Fizzy related protein homolog; Fizzy-related protein homolog; Fizzy/cell division cycle 20 related 1; FYR; FZR 1; FZR 2; Fzr; FZR_HUMAN; Fzr1; Fzr1 protein; FZR2; HCDH 1; HCDH; hCDH1; KIAA1242;
抗原和靶标
Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues.
- Q9UM11 FZR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDQDYERRLLRQIVIQNENTMPRVTEMRRTLTPASSPVSSPSKHGDRFIPSRAGANWSVNFHRINENEKSPSQNRKAKDATSDNGKDGLAYSALLKNELLGAGIEKVQDPQTEDRRLQPSTPEKKGLFTYSLSTKRSSPDDGNDVSPYSLSPVSNKSQKLLRSPRKPTRKISKIPFKVLDAPELQDDFYLNLVDWSSLNVLSVGLGTCVYLWSACTSQVTRLCDLSVEGDSVTSVGWSERGNLVAVGTHKGFVQIWDAAAGKKLSMLEGHTARVGALAWNAEQLSSGSRDRMILQRDIRTPPLQSERRLQGHRQEVCGLKWSTDHQLLASGGNDNKLLVWNHSSLSPVQQYTEHLAAVKAIAWSPHQHGLLASGGGTADRCIRFWNTLTGQPLQCIDTGSQVCNLAWSKHANELVSTHGYSQNQILVWKYPSLTQVAKLTGHSYRVLYLAMSPDGEAIVTGAGDETLRFWNVFSKTRSTKVKWESVSVLNLFTRIR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UM11 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| Y5 | Phosphorylation | Uniprot | |
| T30 | Phosphorylation | Uniprot | |
| T32 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S39 | O-Glycosylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| S40 | O-Glycosylation | Uniprot | |
| S40 | Phosphorylation | P24941 (CDK2) , Q00535 (CDK5) | Uniprot |
| S42 | O-Glycosylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| K69 | Acetylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| Y91 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| T121 | Phosphorylation | P24941 (CDK2) , Q00535 (CDK5) | Uniprot |
| K125 | Sumoylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S154 | Phosphorylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| K159 | Acetylation | Uniprot | |
| S163 | Phosphorylation | Q00535 (CDK5) , P24941 (CDK2) | Uniprot |
| K173 | Ubiquitination | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| T300 | Phosphorylation | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| K475 | Ubiquitination | Uniprot |
研究背景
Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR).
Acetylated. Deacetylated by SIRT2 at Lys-69 and Lys-159; deacetylation enhances the interaction of FZR1 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C).
Phosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint. Phosphorylated by MAK.
Nucleus.
Cytoplasm.
Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues.
The unphosphorylated form interacts with APC/C during mitosis. Interacts with NINL. Interacts (in complex with the anaphase promoting complex APC) with MAD2L2; inhibits FZR1-mediated APC/C activation. Interacts with SIRT2 and USP37. Interacts (via WD repeats) with MAK. Interacts with RBBP8/CtIP; this interaction leads to RBBP8 proteasomal degradation. Interacts with HECW2.
Belongs to the WD repeat CDC20/Fizzy family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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