GNAI2 Antibody - #DF13045

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产品: GNAI2 抗体
货号: DF13045
描述: Rabbit polyclonal antibody to GNAI2
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 40 kDa; 40kD(Calculated).
蛋白号: P04899
RRID: AB_2846006

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 100ul RMB¥ 2300 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(90%), Chicken(100%), Xenopus(80%)
克隆:
Polyclonal
特异性:
GNAI2 Antibody detects endogenous levels of total GNAI2.
RRID:
AB_2846006
引用格式: Affinity Biosciences Cat# DF13045, RRID:AB_2846006.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Adenylate cyclase inhibiting G alpha protein; Adenylate cyclase-inhibiting G alpha protein; GIP; GNAI 2; Gnai2; GNAI2_HUMAN; GNAI2B; GTP binding regulatory protein Gi alpha 2 chain; Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2; Guanine nucleotide-binding protein G(i) subunit alpha-2; H LUCA15.1; H LUCA16.1; WUGSC:H LUCA15.1; WUGSC:H LUCA16.1;

抗原和靶标

免疫原:
Uniprot:

P04899(GNAI2_HUMAN) >>Visit HPA database.

基因/基因ID:
GNAI2(2771)
序列:
MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Dog
90
Xenopus
80
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P04899 作为底物

Site PTM Type Enzyme
G2 Myristoylation
K17 Ubiquitination
K21 Ubiquitination
K29 Ubiquitination
K35 Ubiquitination
S44 Phosphorylation Q02156 (PRKCE)
K46 Ubiquitination
K51 Ubiquitination
K54 Ubiquitination
Y61 Phosphorylation
S62 Phosphorylation
Y69 Phosphorylation
S144 Phosphorylation Q02156 (PRKCE)
Y147 Phosphorylation
Y155 Phosphorylation
Y168 Phosphorylation
K193 Ubiquitination
K198 Ubiquitination
S207 Phosphorylation
K249 Ubiquitination
S253 Phosphorylation
K272 Ubiquitination
K278 Ubiquitination
K296 Ubiquitination
S302 Phosphorylation Q02156 (PRKCE)
Y303 Phosphorylation
K307 Ubiquitination
K313 Ubiquitination
K318 Ubiquitination
Y321 Phosphorylation
K350 Ubiquitination

研究背景

功能:

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.

Regulates the cell surface density of dopamine receptors DRD2 by sequestrating them as an intracellular pool.

翻译修饰:

(Microbial infection) Deamidated at Gln-205 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.

细胞定位:

Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cell membrane. Membrane>Lipid-anchor.
Note: Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1 (By similarity). Interacts with RGS12 and RGS14 (By similarity). Interacts with UNC5B. Interacts (inactive GDP-bound form) with NUCB1 (via GBA motif); the interaction leads to activation of GNAI3 (By similarity). Interacts (inactive GDP-bound form) with CCDC88C/DAPLE (via GBA motif). Interacts (inactive GDP-bound form) with CCDC8A/GIV (via GBA motif).

蛋白家族:

Belongs to the G-alpha family. G(i/o/t/z) subfamily.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Morphine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > GABAergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

· Organismal Systems > Digestive system > Gastric acid secretion.

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