| 产品: | GNAI2 抗体 |
| 货号: | DF13045 |
| 描述: | Rabbit polyclonal antibody to GNAI2 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 40 kDa; 40kD(Calculated). |
| 蛋白号: | P04899 |
| RRID: | AB_2846006 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13045, RRID:AB_2846006.
展开/折叠
Adenylate cyclase inhibiting G alpha protein; Adenylate cyclase-inhibiting G alpha protein; GIP; GNAI 2; Gnai2; GNAI2_HUMAN; GNAI2B; GTP binding regulatory protein Gi alpha 2 chain; Guanine nucleotide binding protein G protein alpha inhibiting activity polypeptide 2; Guanine nucleotide-binding protein G(i) subunit alpha-2; H LUCA15.1; H LUCA16.1; WUGSC:H LUCA15.1; WUGSC:H LUCA16.1;
抗原和靶标
- P04899 GNAI2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P04899 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Myristoylation | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| K46 | Ubiquitination | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| Y61 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| Y69 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| Y147 | Phosphorylation | Uniprot | |
| Y155 | Phosphorylation | Uniprot | |
| Y168 | Phosphorylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| S302 | Phosphorylation | Q02156 (PRKCE) | Uniprot |
| Y303 | Phosphorylation | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| Y321 | Phosphorylation | Uniprot | |
| K350 | Ubiquitination | Uniprot |
研究背景
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.
Regulates the cell surface density of dopamine receptors DRD2 by sequestrating them as an intracellular pool.
(Microbial infection) Deamidated at Gln-205 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.
Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cell membrane. Membrane>Lipid-anchor.
Note: Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.
G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1 (By similarity). Interacts with RGS12 and RGS14 (By similarity). Interacts with UNC5B. Interacts (inactive GDP-bound form) with NUCB1 (via GBA motif); the interaction leads to activation of GNAI3 (By similarity). Interacts (inactive GDP-bound form) with CCDC88C/DAPLE (via GBA motif). Interacts (inactive GDP-bound form) with CCDC8A/GIV (via GBA motif).
Belongs to the G-alpha family. G(i/o/t/z) subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
· Human Diseases > Substance dependence > Cocaine addiction.
· Human Diseases > Substance dependence > Morphine addiction.
· Human Diseases > Substance dependence > Alcoholism.
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Environmental adaptation > Circadian entrainment.
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)
· Organismal Systems > Nervous system > Glutamatergic synapse.
· Organismal Systems > Nervous system > Cholinergic synapse.
· Organismal Systems > Nervous system > Serotonergic synapse.
· Organismal Systems > Nervous system > GABAergic synapse.
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Nervous system > Long-term depression.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Melanogenesis.
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.
· Organismal Systems > Endocrine system > Renin secretion.
· Organismal Systems > Endocrine system > Relaxin signaling pathway.
· Organismal Systems > Digestive system > Gastric acid secretion.
限制条款
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