| 产品: | HERC5 抗体 |
| 货号: | DF13058 |
| 描述: | Rabbit polyclonal antibody to HERC5 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Horse, Sheep |
| 分子量: | 117 kDa; 117kD(Calculated). |
| 蛋白号: | Q9UII4 |
| RRID: | AB_2846019 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13058, RRID:AB_2846019.
展开/折叠
CEB1; CEBP1; Cyclin E binding protein 1; Cyclin-E-binding protein 1; E3 ISG15--protein ligase HERC5; HECT domain and RCC1 like domain containing protein 5; HECT domain and RCC1-like domain-containing protein 5; Hect domain and RLD 5; HECT E3 ubiquitin ligase; HERC5; HERC5_HUMAN; Herc6;
抗原和靶标
Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.
- Q9UII4 HERC5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERRSRRKSRRNGRSTAGKAAATQPAKSPGAQLWLFPSAAGLHRALLRRVEVTRQLCCSPGRLAVLERGGAGVQVHQLLAGSGGARTPKCIKLGKNMKIHSVDQGAEHMLILSSDGKPFEYDNYSMKHLRFESILQEKKIIQITCGDYHSLALSKGGELFAWGQNLHGQLGVGRKFPSTTTPQIVEHLAGVPLAQISAGEAHSMALSMSGNIYSWGKNECGQLGLGHTESKDDPSLIEGLDNQKVEFVACGGSHSALLTQDGLLFTFGAGKHGQLGHNSTQNELRPCLVAELVGYRVTQIACGRWHTLAYVSDLGKVFSFGSGKDGQLGNGGTRDQLMPLPVKVSSSEELKLESHTSEKELIMIAGGNQSILLWIKKENSYVNLKRTIPTLNEGTVKRWIADVETKRWQSTKREIQEIFSSPACLTGSFLRKRRTTEMMPVYLDLNKARNIFKELTQKDWITNMITTCLKDNLLKRLPFHSPPQEALEIFFLLPECPMMHISNNWESLVVPFAKVVCKMSDQSSLVLEEYWATLQESTFSKLVQMFKTAVICQLDYWDESAEENGNVQALLEMLKKLHRVNQVKCQLPESIFQVDELLHRLNFFVEVCRRYLWKMTVDASENVQCCVIFSHFPFIFNNLSKIKLLHTDTLLKIESKKHKAYLRSAAIEEERESEFALRPTFDLTVRRNHLIEDVLNQLSQFENEDLRKELWVSFSGEIGYDLGGVKKEFFYCLFAEMIQPEYGMFMYPEGASCMWFPVKPKFEKKRYFFFGVLCGLSLFNCNVANLPFPLALFKKLLDQMPSLEDLKELSPDLGKNLQTLLDDEGDNFEEVFYIHFNVHWDRNDTNLIPNGSSITVNQTNKRDYVSKYINYIFNDSVKAVYEEFRRGFYKMCDEDIIKLFHPEELKDVIVGNTDYDWKTFEKNARYEPGYNSSHPTIVMFWKAFHKLTLEEKKKFLVFLTGTDRLQMKDLNNMKITFCCPESWNERDPIRALTCFSVLFLPKYSTMETVEEALQEAINNNRGFG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UII4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K19 | Ubiquitination | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| K453 | Ubiquitination | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| K541 | Sumoylation | Uniprot | |
| K643 | Ubiquitination | Uniprot | |
| K652 | Ubiquitination | Uniprot | |
| Y661 | Phosphorylation | Uniprot | |
| K795 | Ubiquitination | Uniprot | |
| K807 | Ubiquitination | Uniprot | |
| S810 | Phosphorylation | Uniprot | |
| K867 | Ubiquitination | Uniprot | |
| Y868 | Phosphorylation | Uniprot | |
| Y871 | Phosphorylation | Uniprot | |
| K890 | Ubiquitination | Uniprot | |
| K898 | Ubiquitination | Uniprot | |
| K906 | Ubiquitination | Uniprot | |
| K918 | Ubiquitination | Uniprot | |
| K946 | Ubiquitination | Uniprot |
研究背景
Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.
ISGylated.
Cytoplasm>Perinuclear region.
Note: Associated with the polyribosomes, probably via the 60S subunit.
Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.
Binds to CCNA1, CCNB1, CCND1 and CCNE1. Interacts with UBE2L6. Interacts with IRF3, this interaction is marginal in resting cells but enhanced upon viral infection. Interacts with influenza A virus NS1.
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