产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13060, RRID:AB_2846021.
展开/折叠
3 hydroxyisobutyryl Coenzyme A hydrolase; 3 hydroxyisobutyryl Coenzyme A hydrolase, mitochondrial; 3-hydroxyisobutyryl-CoA hydrolase; 3-hydroxyisobutyryl-coenzyme A hydrolase; BETA HYDROXYISOBUTYRYL COENZYME A HYDROLASE; HIB CoA hydrolase; HIB-CoA hydrolase; HIBCH; HIBCH_HUMAN; HIBCoA hydrolase; HIBYL CoA H; HIBYL CoAH; HIBYL-CoA-H; HIBYLCoA H; HIBYLCoAH; mitochondrial;
抗原和靶标
Highly expressed in liver and kidney, also detected in heart, muscle and brain (at protein level). Not detected in lung.
- Q6NVY1 HIBCH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGQREMWRLMSRFNAFKRTNTILHHLRMSKHTDAAEEVLLEKKGCTGVITLNRPKFLNALTLNMIRQIYPQLKKWEQDPETFLIIIKGAGGKAFCAGGDIRVISEAEKAKQKIAPVFFREEYMLNNAVGSCQKPYVALIHGITMGGGVGLSVHGQFRVATEKCLFAMPETAIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVYRAGIATHFVDSEKLAMLEEDLLALKSPSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEIIENLQQDGSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACMRGHDFHEGVRAVLIDKDQSPKWKPADLKEVTEEDLNNHFKSLGSSDLKF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q6NVY1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K42 | Ubiquitination | Uniprot | |
| K73 | Acetylation | Uniprot | |
| K92 | Acetylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| Y330 | Phosphorylation | Uniprot | |
| K353 | Acetylation | Uniprot | |
| S356 | Phosphorylation | Uniprot |
研究背景
Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA.
Mitochondrion.
Highly expressed in liver and kidney, also detected in heart, muscle and brain (at protein level). Not detected in lung.
Belongs to the enoyl-CoA hydratase/isomerase family.
研究领域
· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.
· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.
· Metabolism > Carbohydrate metabolism > Propanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
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