| 产品: | LARS 抗体 |
| 货号: | DF13121 |
| 描述: | Rabbit polyclonal antibody to LARS |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 140 kDa; 134kD(Calculated). |
| 蛋白号: | Q9P2J5 |
| RRID: | AB_2846081 |
产品描述
来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
LARS Antibody detects endogenous levels of total LARS.
RRID:
AB_2846081
引用格式: Affinity Biosciences Cat# DF13121, RRID:AB_2846081.
引用格式: Affinity Biosciences Cat# DF13121, RRID:AB_2846081.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
cytoplasmic; Cytoplasmic leucyl tRNA synthetase; Cytosolic leucyl tRNA synthetase; FLJ10595; FLJ21788; hr025Cl; HSPC 192; HSPC192; KIAA1352; LARS 1; Lars; LARS1; Leucine translase; Leucine tRNA ligase; Leucine--tRNA ligase; Leucyl tRNA synthetase cytoplasmic; Leucyl-tRNA synthetase; LeuRS; LEUS; LRS; PIG 44; PIG44; Proliferation inducing gene 44; RNTLS; SYLC_HUMAN;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
序列:
- Q9P2J5 SYLC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAERKGTAKVDFLKKIEKEIQQKWDTERVFEVNASNLEKQTSKGKYFVTFPYPYMNGRLHLGHTFSLSKCEFAVGYQRLKGKCCLFPFGLHCTGMPIKACADKLKREIELYGCPPDFPDEEEEEEETSVKTEDIIIKDKAKGKKSKAAAKAGSSKYQWGIMKSLGLSDEEIVKFSEAEHWLDYFPPLAIQDLKRMGLKVDWRRSFITTDVNPYYDSFVRWQFLTLRERNKIKFGKRYTIYSPKDGQPCMDHDRQTGEGVGPQEYTLLKLKVLEPYPSKLSGLKGKNIFLVAATLRPETMFGQTNCWVRPDMKYIGFETVNGDIFICTQKAARNMSYQGFTKDNGVVPVVKELMGEEILGASLSAPLTSYKVIYVLPMLTIKEDKGTGVVTSVPSDSPDDIAALRDLKKKQALRAKYGIRDDMVLPFEPVPVIEIPGFGNLSAVTICDELKIQSQNDREKLAEAKEKIYLKGFYEGIMLVDGFKGQKVQDVKKTIQKKMIDAGDALIYMEPEKQVMSRSSDECVVALCDQWYLDYGEENWKKQTSQCLKNLETFCEETRRNFEATLGWLQEHACSRTYGLGTHLPWDEQWLIESLSDSTIYMAFYTVAHLLQGGNLHGQAESPLGIRPQQMTKEVWDYVFFKEAPFPKTQIAKEKLDQLKQEFEFWYPVDLRVSGKDLVPNHLSYYLYNHVAMWPEQSDKWPTAVRANGHLLLNSEKMSKSTGNFLTLTQAIDKFSADGMRLALADAGDTVEDANFVEAMADAGILRLYTWVEWVKEMVANWDSLRSGPASTFNDRVFASELNAGIIKTDQNYEKMMFKEALKTGFFEFQAAKDKYRELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH
种属预测
种属预测:
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9P2J5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Ubiquitination | Uniprot | |
| K9 | Ubiquitination | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K45 | Acetylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K193 | Acetylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| Y214 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| K235 | Acetylation | Uniprot | |
| K243 | Acetylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| Y264 | Phosphorylation | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| Y275 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| T367 | Phosphorylation | Uniprot | |
| K381 | Acetylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| K459 | Ubiquitination | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| Y468 | Phosphorylation | Uniprot | |
| Y473 | Phosphorylation | Uniprot | |
| K497 | Ubiquitination | Uniprot | |
| Y507 | Phosphorylation | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| K540 | Acetylation | Uniprot | |
| K541 | Ubiquitination | Uniprot | |
| K548 | Ubiquitination | Uniprot | |
| Y637 | Phosphorylation | Uniprot | |
| K641 | Ubiquitination | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| T648 | Phosphorylation | Uniprot | |
| K659 | Ubiquitination | Uniprot | |
| Y666 | Phosphorylation | Uniprot | |
| T702 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| K716 | Ubiquitination | Uniprot | |
| S718 | Phosphorylation | Uniprot | |
| K719 | Ubiquitination | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| T721 | Phosphorylation | Uniprot | |
| T728 | Phosphorylation | Uniprot | |
| K733 | Ubiquitination | Uniprot | |
| S735 | Phosphorylation | Uniprot | |
| R795 | Methylation | Uniprot | |
| K807 | Ubiquitination | Uniprot | |
| K814 | Ubiquitination | Uniprot | |
| K822 | Ubiquitination | Uniprot | |
| K832 | Ubiquitination | Uniprot | |
| K914 | Ubiquitination | Uniprot | |
| K921 | Acetylation | Uniprot | |
| K923 | Acetylation | Uniprot | |
| K924 | Acetylation | Uniprot | |
| K927 | Ubiquitination | Uniprot | |
| K932 | Ubiquitination | Uniprot | |
| Y944 | Phosphorylation | Uniprot | |
| K965 | Ubiquitination | Uniprot | |
| K970 | Ubiquitination | Uniprot | |
| K983 | Ubiquitination | Uniprot | |
| Y985 | Phosphorylation | Uniprot | |
| K988 | Ubiquitination | Uniprot | |
| K1002 | Ubiquitination | Uniprot | |
| S1042 | Phosphorylation | Uniprot | |
| K1047 | Ubiquitination | Uniprot | |
| K1056 | Ubiquitination | Uniprot | |
| K1133 | Ubiquitination | Uniprot | |
| Y1135 | Phosphorylation | Uniprot | |
| K1138 | Ubiquitination | Uniprot | |
| K1154 | Ubiquitination | Uniprot | |
| Y1173 | Phosphorylation | Uniprot |
研究背景
功能:
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.
细胞定位:
Cytoplasm.
蛋白家族:
Belongs to the class-I aminoacyl-tRNA synthetase family.
研究领域
· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.
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