产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13138, RRID:AB_2846098.
展开/折叠
cytoplasmic; FLJ35667; Mars; Methionine tRNA ligase 1; Methionine tRNA ligase 1 cytoplasmic; Methionine tRNA ligase; Methionine tRNA synthetase; Methionine--tRNA ligase; Methionyl tRNA synthetase cytoplasmic; Methionyl-tRNA synthetase; MetRS; MRS; MTRNS; SYMC_HUMAN;
抗原和靶标
- P56192 SYMC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRLFVSDGVPGCLPVLAAAGRARGRAEVLISTVGPEDCVVPFLTRPKVPVLQLDSGNYLFSTSAICRYFFLLSGWEQDDLTNQWLEWEATELQPALSAALYYLVVQGKKGEDVLGSVRRALTHIDHSLSRQNCPFLAGETESLADIVLWGALYPLLQDPAYLPEELSALHSWFQTLSTQEPCQRAAETVLKQQGVLALRPYLQKQPQPSPAEGRAVTNEPEEEELATLSEEEIAMAVTAWEKGLESLPPLRPQQNPVLPVAGERNVLITSALPYVNNVPHLGNIIGCVLSADVFARYSRLRQWNTLYLCGTDEYGTATETKALEEGLTPQEICDKYHIIHADIYRWFNISFDIFGRTTTPQQTKITQDIFQQLLKRGFVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAVELKKPQCKVCRSCPVVQSSQHLFLDLPKLEKRLEEWLGRTLPGSDWTPNAQFITRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFDATIGYLSITANYTDQWERWWKNPEQVDLYQFMAKDNVPFHSLVFPCSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLLKNNSELLNNLGNFINRAGMFVSKFFGGYVPEMVLTPDDQRLLAHVTLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGSEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCTLPAGHQIGTVSPLFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKKK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P56192 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| Y153 | Phosphorylation | Uniprot | |
| Y161 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Q9P2K8 (EIF2AK4) | Uniprot |
| K335 | Ubiquitination | Uniprot | |
| Y336 | Phosphorylation | Uniprot | |
| Y344 | Phosphorylation | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| K375 | Acetylation | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| R376 | Methylation | Uniprot | |
| S472 | Phosphorylation | Q9P2K8 (EIF2AK4) | Uniprot |
| T482 | Phosphorylation | Uniprot | |
| K500 | Ubiquitination | Uniprot | |
| Y549 | Phosphorylation | Uniprot | |
| S644 | Phosphorylation | Uniprot | |
| S662 | Phosphorylation | Q9P2K8 (EIF2AK4) | Uniprot |
| K663 | Ubiquitination | Uniprot | |
| T675 | Phosphorylation | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| K726 | Acetylation | Uniprot | |
| K726 | Ubiquitination | Uniprot | |
| K729 | Ubiquitination | Uniprot | |
| K823 | Ubiquitination | Uniprot | |
| T824 | Phosphorylation | Uniprot | |
| S825 | Phosphorylation | Uniprot | |
| T833 | Phosphorylation | Uniprot | |
| K838 | Ubiquitination | Uniprot | |
| K875 | Ubiquitination | Uniprot | |
| K881 | Ubiquitination | Uniprot |
研究背景
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Plays a role in the synthesis of ribosomal RNA in the nucleolus.
Cytoplasm>Cytosol. Nucleus>Nucleolus.
Note: Localizes to the nucleolus in proliferative cells but disappears in quiescent cells.
Monomer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 Ref.24,. Forms a linear complex that contains MARS1, EEF1E1, EPRS1 and AIMP2 that is at the core of the multisubunit complex.
Belongs to the class-I aminoacyl-tRNA synthetase family.
研究领域
· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.
· Metabolism > Metabolism of other amino acids > Selenocompound metabolism.
限制条款
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