产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13139, RRID:AB_2846099.
展开/折叠
AdoMet synthase 2; AdoMet synthetase 2; AdoMet synthetase; AMS 2; AMS2; MAT 2; MAT 2A; MAT II; MAT-II; Mat2a; MATA 2; MATA2; MATII; Methionine adenosyltransferase 2; Methionine adenosyltransferase; Methionine adenosyltransferase II alpha; Methionine adenosyltransferase II; METK2_HUMAN; S adenosylmethionine synthetase gamma form; S adenosylmethionine synthetase isoform type 2; S-adenosylmethionine synthase isoform type-2; SAMS 2; SAMS2;
抗原和靶标
- P31153 METK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNGQLNGFHEAFIEEGTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P31153 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K53 | Ubiquitination | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K81 | Acetylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K88 | Acetylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| Y101 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| C104 | S-Nitrosylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| R192 | Methylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K234 | Acetylation | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| Y235 | Phosphorylation | Uniprot | |
| Y242 | Phosphorylation | Uniprot | |
| T262 | Phosphorylation | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| Y320 | Phosphorylation | Uniprot | |
| K340 | Sumoylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| K368 | Sumoylation | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| Y377 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| K394 | Sumoylation | Uniprot |
研究背景
Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
Detected in kidney.
Heterotrimer; composed of a catalytic MAT2A homodimer that binds one regulatory MAT2B chain. Heterohexamer; composed of a central, catalytic MAT2A homotetramer flanked on either side by a regulatory MAT2B chain.
Belongs to the AdoMet synthase family.
研究领域
· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
限制条款
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