MFAP5 Antibody - #DF13146

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产品: MFAP5 抗体
货号: DF13146
描述: Rabbit polyclonal antibody to MFAP5
应用: WB IHC IF/ICC
文献应用: WB
反应: Human, Mouse, Rat
文献反应: Mouse
预测: Pig
分子量: 20 kDa; 20kD(Calculated).
蛋白号: Q13361
RRID: AB_2846106

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(88%)
克隆:
Polyclonal
特异性:
MFAP5 Antibody detects endogenous levels of total MFAP5.
RRID:
AB_2846106
引用格式: Affinity Biosciences Cat# DF13146, RRID:AB_2846106.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AAT9; MAGP 2; MAGP-2; MAGP2; MFAP 5; MFAP-5; MFAP5; MFAP5_HUMAN; Microfibril associated glycoprotein 2; Microfibril-associated glycoprotein 2; microfibrillar associated protein 5; Microfibrillar associated protein 5 precursor; Microfibrillar-associated protein 5; MP25;

抗原和靶标

免疫原:
Uniprot:

Q13361(MFAP5_HUMAN) >>Visit HPA database.

基因/基因ID:
MFAP5(8076)
序列:
MSLLGPKVLLFLAAFIITSDWIPLGVNSQRGDDVTQATPETFTEDPNLVNDPATDETVLAVLADIAPSTDDLASLSEKNTTAECWDEKFTCTRLYSVHRPVKQCIHQLCFTSLRRMYIVNKEICSRLVCKEHEAMKDELCRQMAGLPPRRLRRSNYFRLPPCENVDLQRPNGL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
88
Bovine
75
Sheep
75
Rabbit
75
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13361 作为底物

Site PTM Type Enzyme
T54 O-Glycosylation
N79 N-Glycosylation

研究背景

功能:

May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity (By similarity). Component of the elastin-associated microfibrils.

翻译修饰:

Forms intermolecular disulfide bonds either with other MAGP-2 molecules or with other components of the microfibrils.

N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. O-glycan heterogeneity at Thr-54: HexHexNAc (major) and HexHexNAc + sulfate (minor).

细胞定位:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with TGFB2. Interacts with BMP2. Interacts with FBN1 (via N-terminal domain) and FBN2.

蛋白家族:

Belongs to the MFAP family.

文献引用

1). Microfibrillar-associated protein 5 suppresses adipogenesis by inhibiting essential coactivator of PPARγ. Scientific reports, 2023 (PubMed: 37020143) [IF=3.8]

Application: WB    Species: Mouse    Sample: C3H10 and 3T3-L1 cells

Figure 1 Association of MFAP5 with adipogenic differentiation. (A) Fifty-seven genes were at the intersection of three sequencing datasets before and after adipogenic differentiation. (B) Radar chart of 25 genes with the highest differential expression. Blue and red, down- and up-regulated during adipogenesis, respectively. Multiples of gene expression in each database are indicated by different colors. (C) Oil red O staining of C3H10 and 3T3-L1 cells. (D) Endogenous expression and expression pattern during adipogenesis of MFAP5 in C3H10 and 3T3-L1 cells. (E–G) Relative mRNA levels of MFAP5, CEBPα, FABP4, and SREB1 during adipogenesis. n = 3, the experiment was repeated 3 times. Values are means ± SD. *P 

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