| 产品: | MLN64 抗体 |
| 货号: | DF13153 |
| 描述: | Rabbit polyclonal antibody to MLN64 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 50 kDa; 51kD(Calculated). |
| 蛋白号: | Q14849 |
| RRID: | AB_2846113 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13153, RRID:AB_2846113.
展开/折叠
CAB 1; CAB1; Es 64; Es64; FLJ41370; Metastatic lymph node gene 64 protein; Metastatic lymph node protein 64; MLN 64; Mln64; Protein CAB 1; Protein CAB1; Protein MLN 64; Protein MLN64; StAR related lipid transfer protein 3; StAR-related lipid transfer protein 3; STAR3_HUMAN; STARD 3; StARD3; START domain containing 3; START domain-containing protein 3; Steroidogenic acute regulatory protein related;
抗原和靶标
- Q14849 STAR3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSKLPRELTRDLERSLPAVASLGSSLSHSQSLSSHLLPPPEKRRAISDVRRTFCLFVTFDLLFISLLWIIELNTNTGIRKNLEQEIIQYNFKTSFFDIFVLAFFRFSGLLLGYAVLRLRHWWVIAVTTLVSSAFLIVKVILSELLSKGAFGYLLPIVSFVLAWLETWFLDFKVLPQEAEEERWYLAAQVAVARGPLLFSGALSEGQFYSPPESFAGSDNESDEEVAGKKSFSAQEREYIRQGKEATAVVDQILAQEENWKFEKNNEYGDTVYTIEVPFHGKTFILKTFLPCPAELVYQEVILQPERMVLWNKTVTACQILQRVEDNTLISYDVSAGAAGGVVSPRDFVNVRRIERRRDRYLSSGIATSHSAKPPTHKYVRGENGPGGFIVLKSASNPRVCTFVWILNTDLKGRLPRYLIHQSLAATMFEFAFHLRQRISELGARA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14849 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S15 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| K281 | Ubiquitination | Uniprot |
研究背景
Sterol-binding protein that mediates cholesterol transport from the endoplasmic reticulum to endosomes. Creates contact site between the endoplasmic reticulum and late endosomes: localizes to late endosome membranes and contacts the endoplasmic reticulum via interaction with VAPA and VAPB. Acts as a lipid transfer protein that redirects sterol to the endosome at the expense of the cell membrane and favors membrane formation inside endosomes. May also mediate cholesterol transport between other membranes, such as mitochondria membrane or cell membrane. However, such results need additional experimental evidences; probably mainly mediates cholesterol transport from the endoplasmic reticulum to endosomes. Does not activate transcriptional cholesterol sensing. Able to bind other lipids, such as lutein, a xanthophyll carotenoids that form the macular pigment of the retina.
Late endosome membrane>Multi-pass membrane protein.
Note: Localizes to contact sites between the endoplasmic reticulum and late endosomes: associates with the endoplasmic reticulum membrane via interaction with VAPA and VAPB (PubMed:24105263).
Expressed in retina.
Homodimer. Interacts (via the MENTAL domain) with STARD3NL. Interacts (via FFAT motif) with VAPA. Interacts (via FFAT motif) with VAPB.
The FFAT motif mediates interaction with VAPA and VAPB.
The START domain mediates lipid-transfer between membranes. It contains a hydrophobic cavity able to accommodate one lipid molecule, thereby serving as a 'hydrophobic bridge' across the aqueous gap between donor and acceptor organelle membranes.
The MENTAL domain anchors the protein in endosome membranes and exposes the START domain in the cytosol (PubMed:11053434). It binds cholesterol and mediates homotypic as well as heterotypic interactions between STARD3 and STARD3NL (PubMed:15718238, PubMed:16709157).
Belongs to the STARD3 family.
研究领域
· Organismal Systems > Digestive system > Cholesterol metabolism.
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