| 产品: | NIT2 抗体 |
| 货号: | DF13186 |
| 描述: | Rabbit polyclonal antibody to NIT2 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 30 kDa; 31kD(Calculated). |
| 蛋白号: | Q9NQR4 |
| RRID: | AB_2846146 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13186, RRID:AB_2846146.
展开/折叠
CUA002; MGC111199; NIT 2; Nit protein 2; NIT2; NIT2_HUMAN; Nitrilase family member 2; Nitrilase homolog 2; Omega amidase NIT2; Omega-amidase NIT2;
抗原和靶标
Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.
- Q9NQR4 NIT2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEKIPGESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKAGTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLYAVEMKKP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NQR4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S26 | Phosphorylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| Y49 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K68 | Acetylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| Y145 | Phosphorylation | Uniprot | |
| C146 | S-Nitrosylation | Uniprot | |
| Y165 | Phosphorylation | Uniprot | |
| Y202 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| T237 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| K249 | Acetylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| K250 | Acetylation | Uniprot | |
| R265 | Methylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| Y269 | Phosphorylation | Uniprot |
研究背景
Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.
Cytoplasm.
Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.
Homodimer.
Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
研究领域
· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.
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