NLRP1 Antibody - #DF13187

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产品: NLRP1 抗体
货号: DF13187
描述: Rabbit polyclonal antibody to NLRP1
应用: WB IF/ICC
文献验证: WB
反应: Human
蛋白号: Q9C000
RRID: AB_2846147

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   规格 价格 库存
 50ul RMB¥ 1500 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
NLRP1 Antibody detects endogenous levels of total NLRP1.
RRID:
AB_2846147
引用格式: Affinity Biosciences Cat# DF13187, RRID:AB_2846147.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CARD 7; CARD7; Caspase recruitment domain protein 7; Caspase recruitment domain-containing protein 7; CLR17.1; Death effector filament forming Ced 4 like apoptosis protein; Death effector filament-forming ced-4-like apoptosis protein; DEFCAP; DEFCAP L/S; DKFZp586O1822; KIAA0926; LRR and PYD domains-containing protein 1; NAC alpha/beta/gamma/delta; NAC; NACHT; NACHT leucine rich repeat and PYD containing 1; NACHT leucine rich repeat and PYD pyrin domain containing 1; NACHT leucine rich repeat and pyrin domain containing 1; NACHT LRR and PYD containing protein 1; NALP 1; NALP1; NALP1_HUMAN; NLR family pyrin domain containing 1; NLRP 1; NLRP1; NLRP1 protein; Nucleotide binding domain and caspase recruitment domain; Nucleotide binding oligomerization domain leucine rich repeat and pyrin domain containing 1; Nucleotide-binding domain and caspase recruitment domain; PP 1044; PP1044;

抗原和靶标

免疫原:

A synthesized peptide derived from human NLRP1, corresponding to a region within N-terminal amino acids.

基因/基因ID:
NLRP1(22861)

研究领域

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

文献引用

1). NEMO-Binding Domain/IKKγ Inhibitory Peptide Alleviates Neuronal Pyroptosis in Spinal Cord Injury by Inhibiting ASMase-Induced Lysosome Membrane Permeabilization. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 39225315) [IF=15.1]
2). Cyclic helix B peptide alleviates proinflammatory cell death and improves functional recovery after traumatic spinal cord injury. Redox Biology, 2023 (PubMed: 37290302) [IF=10.7]
3). Elamipretide alleviates pyroptosis in traumatically injured spinal cord by inhibiting cPLA2-induced lysosomal membrane permeabilization. Journal of Neuroinflammation, 2023 (PubMed: 36609266) [IF=9.3]
4). Ginsenoside-Rh2 Promotes Functional Recovery after Spinal Cord Injury by Enhancing TFEB-Mediated Autophagy. Journal of agricultural and food chemistry, 2024 (PubMed: 38907713) [IF=5.7]
5). Sesamin-mediated high expression of BECN2 ameliorates cartilage endplate degeneration by reducing autophagy and inflammation. Aging, 2024 (PubMed: 38284902) [IF=3.9]
6). GDF-11 Protects the Traumatically Injured Spinal Cord by Suppressing Pyroptosis and Necroptosis via TFE3-Mediated Autophagy Augmentation. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34712387)

Application: WB    Species: Mice    Sample: spinal cords

Figure 2 GDF-11 attenuates pyroptosis following spinal cord injury. (a) Immunofluorescence staining for Caspase-1 and NeuN colocalization in the spinal cords of the GDF-11, SCI, and sham groups (scale bar = 25 μm). (b) The quantitative mean optical density of Caspase-1 in neurons of spinal cord lesions. (c) Immunofluorescence staining for GSDMD and NeuN colocalization in the spinal cords of the GDF-11, SCI, and sham groups (scale bar = 25 μm). (d) The quantitative average optical density of GSDMD within neurons of spinal cord lesions. (e) Western blot assay for IL-18, IL-1β, GSDMD, Caspase-1, ASC, NLRP3, and NLRP1 expression levels in the three groups. Gels were subjected to identical experimental conditions, with cropped blots presented. (f) Optical densities of the IL-18, IL-1β, GSDMD, Caspase-1, ASC, NLRP3, and NLRP1 expression levels were quantified and investigated in the respective groups. Data are expressed as the mean ± SEM, n = 6 per group. ∗∗p < 0.01 vs. the sham group. #p < 0.05 and ##p < 0.01 vs. the SCI group.

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