| 产品: | NUDT16 抗体 |
| 货号: | DF13198 |
| 描述: | Rabbit polyclonal antibody to NUDT16 |
| 应用: | WB |
| 反应: | Human |
| 预测: | Pig, Bovine, Horse, Sheep |
| 分子量: | 21 kDa; 21kD(Calculated). |
| 蛋白号: | Q96DE0 |
| RRID: | AB_2846158 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13198, RRID:AB_2846158.
展开/折叠
FLJ31265; FLJ34034; FLJ36248; Nucleoside diphosphate-linked moiety X motif 16; NUD16_HUMAN; Nudix (nucleoside diphosphate linked moiety X) type motif 16; Nudix motif 16; NUDT 16; NUDT16; U8 snoRNA-binding protein H29K; U8 snoRNA-decapping enzyme;
抗原和靶标
- Q96DE0 NUD16_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGARRLELGEALALGSGWRHACHALLYAPDPGMLFGRIPLRYAILMQMRFDGRLGFPGGFVDTQDRSLEDGLNRELREELGEAAAAFRVERTDYRSSHVGSGPRVVAHFYAKRLTLEELLAVEAGATRAKDHGLEVLGLVRVPLYTLRDGVGGLPTFLENSFIGSAREQLLEALQDLGLLQSGSISGLKIPAHH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96DE0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y111 | Phosphorylation | Uniprot | |
| T116 | Phosphorylation | Uniprot | |
| K190 | Ubiquitination | Uniprot |
研究背景
RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms. The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP. Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
Nucleus. Nucleus>Nucleoplasm. Nucleus>Nucleolus. Cytoplasm.
Note: Localized predominantly in the cytoplasm (PubMed:21070968). Localized in nucleolus, and in a minor proportion in distinct foci in the nucleoplasm (By similarity).
Expressed strongly in lung, kidney, adrenal gland, testis, heart and brain.
Homodimer.
Belongs to the Nudix hydrolase family. NUDT16 subfamily.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
· Metabolism > Nucleotide metabolism > Purine metabolism.
限制条款
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