(1)
(4)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13211, RRID:AB_2846230.
展开/折叠
200 kDa neurofilament protein; CMT2CC; Nefh; Neurofilament heavy polypeptide 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; NF H; NF-H; NFH; NFH_HUMAN;
抗原和靶标
- P12036 NFH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGAASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRSAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARVDLQKKAQALQEECGYLRRHHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPIPFSLPEGLPKIPSVSTHIKVKSEEKIKVVEKSEKETVIVEEQTEETQVTEEVTEEEEKEAKEEEGKEEEGGEEEEAEGGEEETKSPPAEEAASPEKEAKSPVKEEAKSPAEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPPEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPAEAKSPEKAKSPVKEEAKSPAEAKSPVKEEAKSPAEVKSPEKAKSPTKEEAKSPEKAKSPEKAKSPEKEEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKTPEKAKSPVKEEAKSPEKAKSPEKAKTLDVKSPEAKTPAKEEARSPADKFPEKAKSPVKEEVKSPEKAKSPLKEDAKAPEKEIPKKEEVKSPVKEEEKPQEVKVKEPPKKAEEEKAPATPKTEEKKDSKKEEAPKKEAPKPKVEEKKEPAVEKPKESKVEAKKEEAEDKKKVPTPEKEAPAKVEVKEDAKPKEKTEVAKKEPDDAKAKEPSKPAEKKEAAPEKKDTKEEKAKKPEEKPKTEAKAKEDDKTLSKEPSKPKAEKAEKSSSTDQKDSKPPEKATEDKAAKGK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P12036 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K31 | Ubiquitination | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| S59 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| Y385 | Phosphorylation | Uniprot | |
| R412 | Methylation | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| T501 | Phosphorylation | Uniprot | |
| S503 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S511 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S526 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| K531 | Ubiquitination | Uniprot | |
| S532 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S540 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| S560 | Phosphorylation | Uniprot | |
| K565 | Ubiquitination | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| S574 | Phosphorylation | Uniprot | |
| S580 | Phosphorylation | Uniprot | |
| S594 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S606 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| S620 | Phosphorylation | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| S634 | Phosphorylation | Uniprot | |
| S640 | Phosphorylation | Uniprot | |
| S648 | Phosphorylation | Uniprot | |
| S654 | Phosphorylation | Uniprot | |
| K657 | Acetylation | Uniprot | |
| K659 | Acetylation | Uniprot | |
| K659 | Ubiquitination | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| K663 | Acetylation | Uniprot | |
| S668 | Phosphorylation | Uniprot | |
| S674 | Phosphorylation | Uniprot | |
| S682 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| S702 | Phosphorylation | Uniprot | |
| S710 | Phosphorylation | Uniprot | |
| S716 | Phosphorylation | Uniprot | |
| S724 | Phosphorylation | Uniprot | |
| S730 | Phosphorylation | Uniprot | |
| T738 | Phosphorylation | Uniprot | |
| S744 | Phosphorylation | Uniprot | |
| S752 | Phosphorylation | Uniprot | |
| S758 | Phosphorylation | Uniprot | |
| S769 | Phosphorylation | Uniprot | |
| S782 | Phosphorylation | Uniprot | |
| S793 | Phosphorylation | Uniprot | |
| S801 | Phosphorylation | Uniprot | |
| S807 | Phosphorylation | Uniprot | |
| S828 | Phosphorylation | Uniprot | |
| K895 | Ubiquitination | Uniprot | |
| K907 | Acetylation | Uniprot | |
| K908 | Acetylation | Uniprot | |
| T911 | Phosphorylation | Uniprot | |
| S948 | Phosphorylation | Uniprot | |
| S993 | Phosphorylation | Uniprot |
研究背景
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Cytoplasm.
Belongs to the intermediate filament family.
研究领域
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
文献引用
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