Collagen X Antibody | Affinity Biosciences LTD|亲科生物官网

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Western blot analysis of extracts from various samples, using Collagen X Antibody.

Western blot analysis of extracts from K562, using Collagen X Antibody.

DF13214 at 1/100 staining Human colorectal cancer by IHC-P.

Figure 2 Paracrine effect of SDSCs toward CCs in indirect coculture.

Figure 2.

Fig.

Figure 4.

FIGURE 3 Phenotypic expression of AUCs during in vitro expansion.

Figure 1 TMF exerts protective effects against OA development.

Figure 2 Effects of TMF on chondrocyte hypertrophy in vitro.

Fig.

产品:	Collagen X 抗体
货号:	DF13214
描述:	Rabbit polyclonal antibody to Collagen X
应用:	WB IHC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken
分子量:	66kDa; 66kD(Calculated).
蛋白号:	Q03692
RRID:	AB_2846233

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
50ul	RMB¥ 1500	现货
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(88%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)

克隆:

Polyclonal

特异性:

Collagen X Antibody detects endogenous levels of total Collagen X.

RRID:

AB_2846233
引用格式: Affinity Biosciences Cat# DF13214, RRID:AB_2846233.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

COAA1_HUMAN; Col10a 1; COL10A1; Collagen alpha 1(X) chain; Collagen alpha-1(X) chain; Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia); Collagen type X alpha 1; Collagen X alpha 1 polypeptide; CollagenX; fa66d11; fb10c08; OTTHUMP00000040411; Procollagen type X alpha 1; Schmid metaphyseal chondrodysplasia; wu:fa66d11; wu:fb10c08;

抗原和靶标

免疫原:

Uniprot:

Q03692(COAA1_HUMAN) >>Visit HPA database.

基因/基因ID:

COL10A1(1300)

序列:

Q03692 COAA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLPQIPFLLLVSLNLVHGVFYAERYQMPTGIKGPLPNTKTQFFIPYTIKSKGIAVRGEQGTPGPPGPAGPRGHPGPSGPPGKPGYGSPGLQGEPGLPGPPGPSAVGKPGVPGLPGKPGERGPYGPKGDVGPAGLPGPRGPPGPPGIPGPAGISVPGKPGQQGPTGAPGPRGFPGEKGAPGVPGMNGQKGEMGYGAPGRPGERGLPGPQGPTGPSGPPGVGKRGENGVPGQPGIKGDRGFPGEMGPIGPPGPQGPPGERGPEGIGKPGAAGAPGQPGIPGTKGLPGAPGIAGPPGPPGFGKPGLPGLKGERGPAGLPGGPGAKGEQGPAGLPGKPGLTGPPGNMGPQGPKGIPGSHGLPGPKGETGPAGPAGYPGAKGERGSPGSDGKPGYPGKPGLDGPKGNPGLPGPKGDPGVGGPPGLPGPVGPAGAKGMPGHNGEAGPRGAPGIPGTRGPIGPPGIPGFPGSKGDPGSPGPPGPAGIATKGLNGPTGPPGPPGPRGHSGEPGLPGPPGPPGPPGQAVMPEGFIKAGQRPSLSGTPLVSANQGVTGMPVSAFTVILSKAYPAIGTPIPFDKILYNRQQHYDPRTGIFTCQIPGIYYFSYHVHVKGTHVWVGLYKNGTPVMYTYDEYTKGYLDQASGSAIIDLTENDQVWLQLPNAESNGLYSSEYVHSSFSGFLVAPM

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Chicken

100

Rabbit

100

Bovine

Xenopus

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

Q03692_COAA1_HUMAN

功能:

Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.

翻译修饰:

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

细胞定位:

Secreted>Extracellular space>Extracellular matrix.

亚基结构:

Homotrimer.

研究领域

· Organismal Systems > Digestive system > Protein digestion and absorption.

文献引用

1). Co-culture pellet of human Wharton’s jelly mesenchymal stem cells and rat costal chondrocytes as a candidate for articular cartilage regeneration: in vitro and in vivo study. Stem Cell Research & Therapy, 2022 (PubMed: 35907866) [IF=7.5]

Application: IHC Species: Rat Sample:

Fig. 3Histological and immunochemistry staining of pellets with semiquantitative analysis. A H–E and Safranin-O staining of pellets and partial enlargement in five groups. B Immunochemistry staining of COLII and COLX of pellets and partial enlargement in five groups. C–E Semiquantitative analysis of AOI of each staining (n = 4). Significant difference symbols: *p < 0.05, **p < 0.01 compared to CCs group, #p < 0.05, ##p < 0.01 compared to hWJMSCs group

2). Anti-hypertrophic effect of synovium-derived stromal cells on costal chondrocytes promotes cartilage repairs. Journal of Orthopaedic Translation, 2022 (PubMed: 34934627) [IF=6.6]

Application: IHC Species: Rat Sample: SDSCs

Figure 2 Paracrine effect of SDSCs toward CCs in indirect coculture. A) Schematic of conditioned medium coculture experimental outline. In light blue: CCs; In yellow: SDSCs; In dark blue: CCs pellet. B) Macroscopic appearance of pellets. C) RT-PCR analysis for chondrogenesis and hypertrophic-related gene expression after 3 weeks of culture. D) H&E staining. E) Alcian blue staining. F, G) Safranin-O staining. H) Biochemical evaluation of GAG content and GAG/DNA ratio of pellets. I, J) Immunohistochemistry analysis of type II collagen. K, L) Immunohistochemistry analysis of type X collagen. M) Western blot analysis of type X collagen. Crtl: Control group. CM: SDSCs-conditioned medium group. (For interpretation of the references to color/colour in this figure legend, the reader is referred to the Web version of this article.)

Application: WB Species: Rat Sample: SDSCs

Application: WB Species: rat Sample: Costal chondrocytes

Figure 2.| Paracrine effect of SDSCs toward CCs in indirect coculture.M) Western blot analysis of type X collagen. Crtl: Control group. CM: SDSCs-conditioned medium group. (For interpretation of the references to color/colour in this figure legend, the reader is referred to the Web version of this article.)

3). Enhanced articular cartilage regeneration using costal chondrocyte-derived scaffold-free tissue engineered constructs with ascorbic acid treatment. Journal of orthopaedic translation, 2024 (PubMed: 38559899) [IF=6.6]

4). Oleic and linoleic acids promote chondrocyte apoptosis by inhibiting autophagy via downregulation of SIRT1/FOXO1 signaling. Biochimica et biophysica acta. Molecular basis of disease, 2024 (PubMed: 38378085) [IF=6.2]

Application: WB Species: Rat Sample:

Fig. 6. Regulation of the expression of extracellular matrix components in chondrocytes by SIRT1 and FOXO1. (A-E) Chondrocytes were transfected with siRNAs against FOXO1 and SIRT1 or with FOXO1- and SIRT1-expressing adenoviruses. (A) Western blot analysis of ADAMTS5, collagen II, and collagen X expression. (B) Quantitative analysis of the protein levels in (A) (n = 3). (C) Real-time PCR analysis of ADAMTS5, collagen II, and collagen X expression. (D) Confocal images of collagen II in chondrocytes (scale bar, 50 μm). (E) Fluorescence intensities of collagen II in (D). Error bars present mean ± SD. * p < 0.05, ** p < 0.01, and *** p < 0.001.

5). Cartilage Regeneration Characteristics of Human and Goat Auricular Chondrocytes. Frontiers in Bioengineering and Biotechnology, 2021 (PubMed: 34993186) [IF=5.7]

Application: IF/ICC Species: human and goat Sample:

FIGURE 3 Phenotypic expression of AUCs during in vitro expansion. (A) Alcian staining, and (B) COL II immunofluorescence staining of human and goats at P1 and P3. Gene quantitative analyses of (C) SOX9, (D) Aggrecan, and (E) Col II in human and goat at P1 and P3. (F–I) Analysis of protein and gene expression levels of COL I and COL X in human and goat at P1 and P3. Data were analyzed using t tests (the relative mRNA levels were compared within the species, instead of comparing between the species). Columns with different letters indicate statistical significance (p < 0.05). Abbreviations: AUCs, auricular chondrocytes; Col II, type II collage; Col I, type I collage; Col X, type X collage. Scale bar = 50 μm.

6). Asiatic acid attenuates hypertrophic and fibrotic differentiation of articular chondrocytes via AMPK/PI3K/AKT signaling pathway. ARTHRITIS RESEARCH & THERAPY, 2020 (PubMed: 32398124) [IF=4.9]

7). Region-specific effects of blocking estrogen receptors on longitudinal bone growth. JOURNAL OF ENDOCRINOLOGY, 2021 (PubMed: 34014834) [IF=4.0]

8). Biomechanism of abnormal stress on promoting osteoarthritis of temporomandibular joint through Piezo1 ion channel. Journal of oral rehabilitation, 2024 (PubMed: 38873703) [IF=2.9]

9). TMF suppresses chondrocyte hypertrophy in osteoarthritic cartilage by mediating the FOXO3a/BMPER pathway. Experimental and therapeutic medicine, 2024 (PubMed: 38800044) [IF=2.7]

Application: IHC Species: Rat Sample:

Figure 1 TMF exerts protective effects against OA development. (A) Overall observation of joint cartilage in rats. (B) H&E staining of cartilage. Immunohistochemical analysis of (C) Col X and (E) Runx2 expression levels. Immunofluorescent intensity of (D) collagen X and (F) Runx2. The arrows indicate positive staining. *P

Application: WB Species: Rat Sample:

Figure 2 Effects of TMF on chondrocyte hypertrophy in vitro. Protein expression levels of (A and B) Col X, (A and C) Runx2, (A and D) BMPER, (A and E) BMP4 and (A and F) p-Smad1/Smad1. Immunofluorescent intensity of (G) Col X and (H) Runx2. *P

10). Wnt/PCP-YAP-BIRC2 axis maintains cartilage stem/progenitor cell homeostasis in osteoarthritis. Research Square, 2022

Application: WB Species: Human Sample: CSPCs

Figure 4. YAP maintains the function of CSPCs a the protein levels of FAK, Lubricin, and YAP in OA-CSPCs (left) and Col II, Col I, and Col X in OA-chondrocytes (right) that co-cultured for 7 days. OA-CSPCs overexpressing or silencing YAP were evaluated by EdU proliferation assay (b), transwell migration assay (c), and SA-β-Gal staining (d). The cells were counted in five random fields per well. Bars = 100 μm.

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Collagen X Antibody - #DF13214

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