产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13215, RRID:AB_2846234.
展开/折叠
Adapter related protein complex 1 beta 1 subunit; Adaptor Protein Complex AP1 Beta 1 Subunit; ADTB1; AP 1 complex subunit beta 1; AP105A; AP1B1; BAM22; Beta Adaptin 1; Beta Prime Adaptin; CLAPB2; Clathrin Assembly Protein Complex 1 Beta Large Chain; Clathrin heavy chain 1; CLH 17; CLH17; CLTC; CLTCL2; Golgi Adaptor HA1/AP1 Adaptin Beta Subunit; KIAA0034; Plasma Membrane Adaptor HA2/AP2 Adaptor Beta Subunit;
抗原和靶标
- Q00610 CLH1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANTSGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYRAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q00610 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T31 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| T105 | Phosphorylation | Uniprot | |
| R144 | Methylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| C151 | S-Nitrosylation | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| K227 | Acetylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| T235 | Phosphorylation | Uniprot | |
| T238 | Phosphorylation | Uniprot | |
| K245 | Acetylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K367 | Acetylation | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| Y377 | Phosphorylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| T397 | Phosphorylation | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| T411 | Phosphorylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| Y430 | Phosphorylation | Uniprot | |
| K450 | Acetylation | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| K453 | Ubiquitination | Uniprot | |
| K456 | Acetylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| T473 | Phosphorylation | Uniprot | |
| C491 | S-Nitrosylation | Uniprot | |
| K500 | Ubiquitination | Uniprot | |
| K506 | Acetylation | Uniprot | |
| K507 | Ubiquitination | Uniprot | |
| Y510 | Phosphorylation | Uniprot | |
| Y556 | Phosphorylation | Uniprot | |
| K619 | Methylation | Uniprot | |
| K619 | Ubiquitination | Uniprot | |
| Y634 | Phosphorylation | Uniprot | |
| K637 | Acetylation | Uniprot | |
| K637 | Ubiquitination | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| C736 | S-Nitrosylation | Uniprot | |
| K737 | Ubiquitination | Uniprot | |
| K742 | Acetylation | Uniprot | |
| K742 | Ubiquitination | Uniprot | |
| S751 | Phosphorylation | Uniprot | |
| K760 | Ubiquitination | Uniprot | |
| K764 | Acetylation | Uniprot | |
| K764 | Ubiquitination | Uniprot | |
| K798 | Ubiquitination | Uniprot | |
| Y799 | Phosphorylation | Uniprot | |
| Y803 | Phosphorylation | Uniprot | |
| K806 | Ubiquitination | Uniprot | |
| S825 | Phosphorylation | Uniprot | |
| S841 | Phosphorylation | Uniprot | |
| T842 | Phosphorylation | Uniprot | |
| K851 | Ubiquitination | Uniprot | |
| K856 | Acetylation | Uniprot | |
| K856 | Ubiquitination | Uniprot | |
| K881 | Ubiquitination | Uniprot | |
| Y883 | Phosphorylation | Uniprot | |
| Y899 | Phosphorylation | Uniprot | |
| Y900 | Phosphorylation | Uniprot | |
| S902 | Phosphorylation | Uniprot | |
| R903 | Methylation | Uniprot | |
| K907 | Acetylation | Uniprot | |
| K907 | Ubiquitination | Uniprot | |
| K911 | Ubiquitination | Uniprot | |
| C918 | S-Nitrosylation | Uniprot | |
| C926 | S-Nitrosylation | Uniprot | |
| C934 | S-Nitrosylation | Uniprot | |
| S938 | Phosphorylation | Uniprot | |
| K941 | Ubiquitination | Uniprot | |
| S1016 | Phosphorylation | Uniprot | |
| S1019 | Phosphorylation | Uniprot | |
| K1034 | Ubiquitination | Uniprot | |
| K1074 | Ubiquitination | Uniprot | |
| Y1096 | Phosphorylation | Uniprot | |
| C1102 | S-Nitrosylation | Uniprot | |
| S1109 | Phosphorylation | Uniprot | |
| K1118 | Ubiquitination | Uniprot | |
| K1122 | Ubiquitination | Uniprot | |
| K1130 | Ubiquitination | Uniprot | |
| Y1137 | Phosphorylation | Uniprot | |
| K1155 | Acetylation | Uniprot | |
| S1167 | Phosphorylation | Uniprot | |
| K1179 | Ubiquitination | Uniprot | |
| T1180 | Phosphorylation | Uniprot | |
| Y1206 | Phosphorylation | Uniprot | |
| K1209 | Acetylation | Uniprot | |
| K1209 | Ubiquitination | Uniprot | |
| Y1211 | Phosphorylation | Uniprot | |
| K1215 | Ubiquitination | Uniprot | |
| Y1218 | Phosphorylation | Uniprot | |
| S1222 | Phosphorylation | Uniprot | |
| R1226 | Methylation | Uniprot | |
| S1229 | Phosphorylation | Uniprot | |
| T1230 | Phosphorylation | Uniprot | |
| Y1237 | Phosphorylation | Uniprot | |
| K1254 | Ubiquitination | Uniprot | |
| K1264 | Acetylation | Uniprot | |
| K1264 | Ubiquitination | Uniprot | |
| T1318 | Phosphorylation | Uniprot | |
| S1341 | Phosphorylation | Uniprot | |
| K1347 | Ubiquitination | Uniprot | |
| K1387 | Ubiquitination | Uniprot | |
| K1392 | Ubiquitination | Uniprot | |
| T1396 | Phosphorylation | Uniprot | |
| K1397 | Ubiquitination | Uniprot | |
| Y1404 | Phosphorylation | Uniprot | |
| Y1405 | Phosphorylation | Uniprot | |
| Y1438 | Phosphorylation | Uniprot | |
| S1440 | Phosphorylation | Uniprot | |
| K1441 | Acetylation | Uniprot | |
| K1441 | Methylation | Uniprot | |
| K1441 | Ubiquitination | Uniprot | |
| K1443 | Ubiquitination | Uniprot | |
| K1449 | Acetylation | Uniprot | |
| K1449 | Ubiquitination | Uniprot | |
| K1461 | Ubiquitination | Uniprot | |
| S1462 | Phosphorylation | Uniprot | |
| S1466 | Phosphorylation | Uniprot | |
| Y1477 | Phosphorylation | P12931 (SRC) | Uniprot |
| T1482 | Phosphorylation | Uniprot | |
| Y1487 | Phosphorylation | Uniprot | |
| S1494 | Phosphorylation | Uniprot | |
| K1501 | Acetylation | Uniprot | |
| K1501 | Ubiquitination | Uniprot | |
| Y1513 | Phosphorylation | Uniprot | |
| K1516 | Acetylation | Uniprot | |
| K1522 | Ubiquitination | Uniprot | |
| S1524 | Phosphorylation | Uniprot | |
| K1529 | Ubiquitination | Uniprot | |
| K1535 | Acetylation | Uniprot | |
| K1535 | Ubiquitination | Uniprot | |
| S1544 | Phosphorylation | Uniprot | |
| T1608 | Phosphorylation | Uniprot | |
| K1609 | Ubiquitination | Uniprot | |
| K1612 | Ubiquitination | Uniprot | |
| S1618 | Phosphorylation | Uniprot | |
| K1621 | Acetylation | Uniprot | |
| Y1634 | Phosphorylation | Uniprot | |
| Y1657 | Phosphorylation | Uniprot | |
| Y1659 | Phosphorylation | Uniprot | |
| T1660 | Phosphorylation | Uniprot | |
| Y1664 | Phosphorylation | Uniprot | |
| Y1673 | Phosphorylation | Uniprot | |
| S1674 | Phosphorylation | Uniprot |
研究背景
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation.
Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Coated pit>Peripheral membrane protein>Cytoplasmic side. Melanosome. Cytoplasm>Cytoskeleton>Spindle.
Note: Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In complex with TACC3 and CKAP5 (forming the TACC3/ch-TOG/clathrin complex) localized to inter-microtubule bridges in mitotic spindles.
Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. Interacts with HIP1. Interacts with DENND1A, DENND1B and DENND1C (By similarity). May interact with OCRL (By similarity). Interacts with ERBB2. Interacts with FKBP6. Interacts with CKAP5 and TACC3 forming the TACC3/ch-TOG/clathrin complex located at spindle inter-microtubules bridges; the complex implicates clathrin triskelions; TACC3 and CLTC are proposed to form a composite microtubule interaction surface. Interacts with ATG16L1 (via N-terminus). Interacts with RFTN1; the interaction occurs in response to pathogens. Interacts with USP2 isoform 4.
The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It constitutes a major protein-protein interaction node.
Belongs to the clathrin heavy chain family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Organismal Systems > Nervous system > Synaptic vesicle cycle.
· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.
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