产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13217, RRID:AB_2846236.
展开/折叠
Cardiac tetrodotoxin insensitive voltage dependent sodium channel alpha subunit; CDCD2; CMD1E; CMPD2; HB1; HB2; HBBD; HH1; ICCD; IVF; LQT3; PFHB1; Scn5a (gene name); Scn5a; SCN5A_HUMAN; Sodium channel protein cardiac muscle alpha subunit; Sodium channel protein cardiac muscle subunit alpha; Sodium channel protein type 5 subunit alpha; Sodium channel protein type V alpha subunit; Sodium channel protein type V subunit alpha; SSS1; VF1; Voltage gated sodium channel alpha subunit Nav1.5; Voltage-gated sodium channel subunit alpha Nav1.5;
抗原和靶标
Found in jejunal circular smooth muscle cells (at protein level). Expressed in human atrial and ventricular cardiac muscle but not in adult skeletal muscle, brain, myometrium, liver, or spleen. Isoform 4 is expressed in brain.
- Q14524 SCN5A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSTTLQESREGLPEEEAPRPQLDLQASKKLPDLYGNPPQELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPIRRAAVKILVHSLFNMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTALNGTNGSVEADGLVWESLDLYLSDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVNSHERRSKRRKRMSSGTEECGEDRLPKSDSEDGPRAMNHLSLTRGLSRTSMKPRSSRGSIFTFRRRDLGSEADFADDENSTAGESESHHTSLLVPWPLRRTSAQGQPSPGTSAPGHALHGKKNSTVDCNGVVSLLGAGDPEATSPGSHLLRPVMLEHPPDTTTPSEEPGGPQMLTSQAPCVDGFEEPGARQRALSAVSVLTSALEELEESRHKCPPCWNRLAQRYLIWECCPLWMSIKQGVKLVVMDPFTDLTITMCIVLNTLFMALEHYNMTSEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMSNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRDSDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDREMNNLQLALARIQRGLRFVKRTTWDFCCGLLRQRPQKPAALAAQGQLPSCIATPYSPPPPETEKVPPTRKETRFEEGEQPGQGTPGDPEPVCVPIAVAESDTDDQEEDEENSLGTEEESSKQQESQPVSGGPEAPPDSRTWSQVSATASSEAEASASQADWRQQWKAEPQAPGCGETPEDSCSEGSTADMTNTAELLEQIPDLGQDVKDPEDCFTEGCVRRCPCCAVDTTQAPGKVWWRLRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSQCESLNLTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKINILAKINLLFVAIFTGECIVKLAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPTLPNSNGSRGDCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYSVLSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSAMVIQRAFRRHLLQRSLKHASFLFRQQAGSGLSEEDAPEREGLIAYVMSENFSRPLGPPSSSSISSTSFPPSYDSVTRATSDNLQVRGSDYSHSEDLADFPPSPDRDRESIV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14524 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S11 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| T17 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| T38 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| K343 | Acetylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| T486 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| R513 | Methylation | Uniprot | |
| S516 | Phosphorylation | Q13557 (CAMK2D) | Uniprot |
| S519 | Phosphorylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| R526 | Methylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| T531 | Phosphorylation | Uniprot | |
| S539 | Phosphorylation | Uniprot | |
| T570 | Phosphorylation | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| S577 | Phosphorylation | Uniprot | |
| K590 | Ubiquitination | Uniprot | |
| T594 | Phosphorylation | Q13557 (CAMK2D) | Uniprot |
| S664 | Phosphorylation | Uniprot | |
| S667 | Phosphorylation | Uniprot | |
| R680 | Methylation | Uniprot | |
| Y694 | Phosphorylation | Uniprot | |
| S1003 | Phosphorylation | Uniprot | |
| T1094 | Phosphorylation | Uniprot | |
| S1103 | Phosphorylation | Uniprot | |
| S1104 | Phosphorylation | Uniprot | |
| K1479 | Acetylation | Uniprot | |
| K1493 | Acetylation | Uniprot | |
| Y1494 | Phosphorylation | P06241 (FYN) | Uniprot |
| Y1495 | Phosphorylation | P06241 (FYN) | Uniprot |
| S1503 | Phosphorylation | Uniprot | |
| S1865 | Phosphorylation | Uniprot | |
| S1885 | Phosphorylation | Uniprot | |
| T1895 | Phosphorylation | Uniprot | |
| S1920 | Phosphorylation | Uniprot | |
| S1925 | Phosphorylation | Uniprot | |
| S1934 | Phosphorylation | Uniprot | |
| S1937 | Phosphorylation | Uniprot | |
| S1985 | Phosphorylation | Uniprot | |
| S1998 | Phosphorylation | Uniprot | |
| S2007 | Phosphorylation | Uniprot |
研究背景
This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant Na(+) channel isoform. This channel is responsible for the initial upstroke of the action potential. Channel inactivation is regulated by intracellular calcium levels.
Ubiquitinated by NEDD4L; which promotes its endocytosis. Does not seem to be ubiquitinated by NEDD4 or WWP2.
Phosphorylation at Ser-1503 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents (Probable). Regulated through phosphorylation by CaMK2D (By similarity).
Lacks the cysteine which covalently binds the conotoxin GVIIJ. This cysteine (position 868) is speculated in other sodium channel subunits alpha to be implied in covalent binding with the sodium channel subunit beta-2 or beta-4.
Cell membrane>Multi-pass membrane protein. Cytoplasm>Perinuclear region. Cell membrane>Sarcolemma>T-tubule.
Note: RANGRF promotes trafficking to the cell membrane.
Found in jejunal circular smooth muscle cells (at protein level). Expressed in human atrial and ventricular cardiac muscle but not in adult skeletal muscle, brain, myometrium, liver, or spleen. Isoform 4 is expressed in brain.
Interacts with the PDZ domain of the syntrophin SNTA1, SNTB1 and SNTB2 (By similarity). Interacts with NEDD4, NEDD4L, WWP2 and GPD1L. Interacts with CALM. Interacts with FGF13; the interaction is direct and FGF13 may regulate SNC5A density at membranes and function. May also interact with FGF12 and FGF14. Interacts with the spider Jingzhaotoxin-I (AC P83974, AC B1P1B7, AC B1P1B8). Interacts with ANK3.
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1, S2, S3, S5, S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
The IQ domain mediates association with calmodulin.
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.5/SCN5A subfamily.
研究领域
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
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