产品: Carbonic Anhydrase IX 抗体
货号: DF13218
描述: Rabbit polyclonal antibody to Carbonic Anhydrase IX
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 49kDa; 50kD(Calculated).
蛋白号: Q16790
RRID: AB_2846237

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
克隆:
Polyclonal
特异性:
Carbonic Anhydrase IX Antibody detects endogenous levels of total Carbonic Anhydrase IX.
RRID:
AB_2846237
引用格式: Affinity Biosciences Cat# DF13218, RRID:AB_2846237.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CA-IX; CA9; CAH9_HUMAN; CAIX; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; Carbonic dehydratase; G250; Membrane antigen MN; MN; P54/58N; pMW1; RCC associated protein G250; RCC-associated antigen G250; Renal cell carcinoma-associated antigen G250;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q16790 CAH9_HUMAN:

Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

序列:
MAPLCPSPWLPLLIPAPAPGLTVQLLLSLLLLVPVHPQRLPRMQEDSPLGGGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNSCLAAGDILALVFGLLFAVTSVAFLVQMRRQHRRGTKGGVSYRPAEVAETGA

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q16790 作为底物

Site PTM Type Enzyme
T115 O-Glycosylation
Y289 Phosphorylation
T333 Phosphorylation
N346 N-Glycosylation
T443 Phosphorylation P17612 (PRKACA)
S448 Phosphorylation
Y449 Phosphorylation

研究背景

功能:

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

翻译修饰:

Asn-346 bears high-mannose type glycan structures.

细胞定位:

Nucleus. Nucleus>Nucleolus. Cell membrane>Single-pass type I membrane protein. Cell projection>Microvillus membrane>Single-pass type I membrane protein.
Note: Found on the surface microvilli and in the nucleus, particularly in nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

亚基结构:

Forms oligomers linked by disulfide bonds.

蛋白家族:

Belongs to the alpha-carbonic anhydrase family.

研究领域

· Metabolism > Energy metabolism > Nitrogen metabolism.

文献引用

1). Platelets are recruited to hepatocellular carcinoma tissues in a CX3CL1‐CX3CR1 dependent manner and induce tumour cell apoptosis. Molecular Oncology (PubMed: 32799418) [IF=6.6]

Application: IHC    Species: human    Sample: liver

Fig. 4. |Hypoxia promotes platelet recruitment by upregulating CX3CL1 expression.(C) Expression of CX3CL1 and CA9 in human HCC and NT liver tissues. Representative images of two consecutive slices of the same sample embedded in paraffin were taken, and each was stained with CX3CL1 and CA9 (n = 3). Bar, 200 lm.

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