产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13219, RRID:AB_2846238.
展开/折叠
35 kDa; arginine/serine-rich 2; PR264; Protein PR264; SC 35; SC-35; SC35; Serine/arginine-rich splicing factor 2; SFRS 2; SFRS2; SFRS2A; Splicing component 35 kDa; Splicing component; Splicing factor; Splicing factor arginine/serine rich 2; Splicing factor SC35; Splicing speckle; Splicing speckles; SR splicing factor 2; SRp30b; SRSF2; SRSF2_HUMAN;
抗原和靶标
- Q01130 SRSF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSYGRPPPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRSPRRRRRSRSRSRSRSRSRSRSRYSRSKSRSRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRSPPPVSKRESKSRSRSKSPPKSPEEEGAVSS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q01130 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| R5 | Methylation | Uniprot | |
| T22 | Phosphorylation | Uniprot | |
| Y23 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| T29 | Phosphorylation | Uniprot | |
| R31 | Methylation | Uniprot | |
| K36 | Acetylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| R39 | Methylation | Uniprot | |
| Y44 | Phosphorylation | Uniprot | |
| K52 | Acetylation | Uniprot | |
| R55 | Methylation | Uniprot | |
| R66 | Methylation | Uniprot | |
| R83 | Methylation | Uniprot | |
| Y92 | Phosphorylation | Uniprot | |
| R94 | Methylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| R103 | Methylation | Uniprot | |
| R109 | Methylation | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S128 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S130 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S142 | Phosphorylation | Uniprot | |
| Y144 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| S157 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K172 | Methylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot |
研究背景
Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment.
Extensively phosphorylated on serine residues in the RS domain. Phosphorylated by SRPK2 and this causes its redistribution from the nuclear speckle to nucleoplasm and controls cell fate decision in response to cisplatin treatment. KAT5/TIP60 inhibits its phosphorylation by preventing SRPK2 nuclear translocation.
Acetylation on Lys-52 by KAT5/TIP60 promotes its proteasomal degradation. This effect is counterbalanced by HDAC6, which positively controls SRSF2 protein level by deacetylating it and preventing its proteasomal degradation.
Nucleus. Nucleus>Nucleoplasm. Nucleus speckle.
Note: Phosphorylation by SRPK2 provokes its redistribution from the nuclear specke to nucleoplasm.
Interacts with BRDT (By similarity). In vitro, self-associates and binds SRSF1/SFRS1 (ASF/SF2), SNRNP70 and U2AF1 but not U2AF2. Binds SREK1/SFRS12. Interacts with CCNL1 and CCNL2. Interacts with SCAF11. Interacts with ZRSR2/U2AF1-RS2. Interacts with CCDC55 (via C-terminus).
Belongs to the splicing factor SR family.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.