产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13223, RRID:AB_2846242.
抗原和靶标
Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues.
- P53675 CLH2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQILPVRFQEHFQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVTIIDMSDPMAPIRRPISAESAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMAEEVIFWKWVSVNTVALVTETAVYHWSMEGDSQPMKMFDRHTSLVGCQVIHYRTDEYQKWLLLVGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFAEFKMEGNAKPATLFCFAVRNPTGGKLHIIEVGQPAAGNQPFVKKAVDVFFPPEAQNDFPVAMQIGAKHGVIYLITKYGYLHLYDLESGVCICMNRISADTIFVTAPHKPTSGIIGVNKKGQVLSVCVEEDNIVNYATNVLQNPDLGLRLAVRSNLAGAEKLFVRKFNTLFAQGSYAEAAKVAASAPKGILRTRETVQKFQSIPAQSGQASPLLQYFGILLDQGQLNKLESLELCHLVLQQGRKQLLEKWLKEDKLECSEELGDLVKTTDPMLALSVYLRANVPSKVIQCFAETGQFQKIVLYAKKVGYTPDWIFLLRGVMKISPEQGLQFSRMLVQDEEPLANISQIVDIFMENSLIQQCTSFLLDALKNNRPAEGLLQTWLLEMNLVHAPQVADAILGNKMFTHYDRAHIAQLCEKAGLLQQALEHYTDLYDIKRAVVHTHLLNPEWLVNFFGSLSVEDSVECLHAMLSANIRQNLQLCVQVASKYHEQLGTQALVELFESFKSYKGLFYFLGSIVNFSQDPDVHLKYIQAACKTGQIKEVERICRESSCYNPERVKNFLKEAKLTDQLPLIIVCDRFGFVHDLVLYLYRNNLQRYIEIYVQKVNPSRTPAVIGGLLDVDCSEEVIKHLIMAVRGQFSTDELVAEVEKRNRLKLLLPWLESQIQEGCEEPATHNALAKIYIDSNNSPECFLRENAYYDSSVVGRYCEKRDPHLACVAYERGQCDLELIKVCNENSLFKSEARYLVCRKDPELWAHVLEETNPSRRQLIDQVVQTALSETRDPEEISVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYISRLDNYDALDIASIAVSSALYEEAFTVFHKFDMNASAIQVLIEHIGNLDRAYEFAERCNEPAVWSQLAQAQLQKDLVKEAINSYIRGDDPSSYLEVVQSASRSNNWEDLVKFLQMARKKGRESYIETELIFALAKTSRVSELEDFINGPNNAHIQQVGDRCYEEGMYEAAKLLYSNVSNFARLASTLVHLGEYQAAVDNSRKASSTRTWKEVCFACMDGQEFRFAQLCGLHIVIHADELEELMCYYQDRGYFEELILLLEAALGLERAHMGMFTELAILYSKFKPQKMLEHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAVLTMMSHPTEAWKEGQFKDIITKVANVELCYRALQFYLDYKPLLINDLLLVLSPRLDHTWTVSFFSKAGQLPLVKPYLRSVQSHNNKSVNEALNHLLTEEEDYQGLRASIDAYDNFDNISLAQQLEKHQLMEFRCIAAYLYKGNNWWAQSVELCKKDHLYKDAMQHAAESRDAELAQKLLQWFLEEGKRECFAACLFTCYDLLRPDMVLELAWRHNLVDLAMPYFIQVMREYLSKVDKLDALESLRKQEEHVTEPAPLVFDFDGHE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P53675 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K78 | Ubiquitination | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| T103 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| T214 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| K450 | Acetylation | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| K453 | Ubiquitination | Uniprot | |
| K456 | Acetylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| S477 | Phosphorylation | Uniprot | |
| K506 | Acetylation | Uniprot | |
| K507 | Ubiquitination | Uniprot | |
| Y510 | Phosphorylation | Uniprot | |
| K619 | Ubiquitination | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| K737 | Ubiquitination | Uniprot | |
| K742 | Acetylation | Uniprot | |
| K742 | Ubiquitination | Uniprot | |
| Y754 | Phosphorylation | Uniprot | |
| K764 | Acetylation | Uniprot | |
| K764 | Ubiquitination | Uniprot | |
| Y799 | Phosphorylation | Uniprot | |
| Y803 | Phosphorylation | Uniprot | |
| K806 | Ubiquitination | Uniprot | |
| S841 | Phosphorylation | Uniprot | |
| T842 | Phosphorylation | Uniprot | |
| K851 | Ubiquitination | Uniprot | |
| K856 | Ubiquitination | Uniprot | |
| S889 | Phosphorylation | Uniprot | |
| K911 | Ubiquitination | Uniprot | |
| K932 | Ubiquitination | Uniprot | |
| K941 | Ubiquitination | Uniprot | |
| K951 | Ubiquitination | Uniprot | |
| T963 | Phosphorylation | Uniprot | |
| S966 | Phosphorylation | Uniprot | |
| S989 | Phosphorylation | Uniprot | |
| S1016 | Phosphorylation | Uniprot | |
| S1019 | Phosphorylation | Uniprot | |
| K1034 | Ubiquitination | Uniprot | |
| T1038 | Phosphorylation | Uniprot | |
| Y1043 | Phosphorylation | Uniprot | |
| S1045 | Phosphorylation | Uniprot | |
| Y1096 | Phosphorylation | Uniprot | |
| K1122 | Ubiquitination | Uniprot | |
| Y1168 | Phosphorylation | Uniprot | |
| T1171 | Phosphorylation | Uniprot | |
| S1181 | Phosphorylation | Uniprot | |
| T1318 | Phosphorylation | Uniprot | |
| S1341 | Phosphorylation | Uniprot | |
| K1347 | Ubiquitination | Uniprot | |
| K1387 | Ubiquitination | Uniprot | |
| K1392 | Ubiquitination | Uniprot | |
| T1396 | Phosphorylation | Uniprot | |
| K1449 | Acetylation | Uniprot | |
| K1449 | Ubiquitination | Uniprot | |
| K1461 | Ubiquitination | Uniprot | |
| S1462 | Phosphorylation | Uniprot | |
| K1529 | Ubiquitination | Uniprot | |
| K1535 | Ubiquitination | Uniprot | |
| K1612 | Ubiquitination | Uniprot | |
| K1621 | Ubiquitination | Uniprot |
研究背景
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network (By similarity).
Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Coated pit>Peripheral membrane protein>Cytoplasmic side.
Note: Cytoplasmic face of coated pits and vesicles.
Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues.
Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium (By similarity). May interact with OCRL (By similarity).
The C-terminal third of the heavy chains forms the hub of the triskelion. This region contains the trimerization domain and the light-chain binding domain involved in the assembly of the clathrin lattice.
The N-terminal seven-bladed beta-propeller is formed by WD40-like repeats, and projects inward from the polyhedral outer clathrin coat. It constitutes a major protein-protein interaction node (By similarity).
Belongs to the clathrin heavy chain family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Organismal Systems > Nervous system > Synaptic vesicle cycle.
· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.
限制条款
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