产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13228, RRID:AB_2846247.
展开/折叠
dJ355L5.1 (prolyl endopeptidase); HGNC:9358; MGC16060; OTTHUMP00000040498; PE; PEP; Post proline cleaving enzyme; Post-proline cleaving enzyme; PPCE_HUMAN; Prep; Prolyl endopeptidase; Prolyl oligopeptidase;
抗原和靶标
- P48147 PPCE_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLSLQYPDVYRDETAVQDYHGHKICDPYAWLEDPDSEQTKAFVEAQNKITVPFLEQCPIRGLYKERMTELYDYPKYSCHFKKGKRYFYFYNTGLQNQRVLYVQDSLEGEARVFLDPNILSDDGTVALRGYAFSEDGEYFAYGLSASGSDWVTIKFMKVDGAKELPDVLERVKFSCMAWTHDGKGMFYNSYPQQDGKSDGTETSTNLHQKLYYHVLGTDQSEDILCAEFPDEPKWMGGAELSDDGRYVLLSIREGCDPVNRLWYCDLQQESSGIAGILKWVKLIDNFEGEYDYVTNEGTVFTFKTNRQSPNYRVINIDFRDPEESKWKVLVPEHEKDVLEWIACVRSNFLVLCYLHDVKNILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRHMGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTSPKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAWTTDYGCSDSKQHFEWLVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNVDWIP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P48147 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| Y19 | Phosphorylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| Y28 | Phosphorylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| Y71 | Phosphorylation | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| Y90 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| K157 | Acetylation | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| Y246 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| Y435 | Phosphorylation | Uniprot | |
| Y442 | Phosphorylation | Uniprot | |
| Y510 | Phosphorylation | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| K539 | Ubiquitination | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| T596 | Phosphorylation | Uniprot | |
| S615 | Phosphorylation | Uniprot | |
| K621 | Ubiquitination | Uniprot | |
| R643 | Methylation | Uniprot | |
| Y658 | Phosphorylation | Uniprot | |
| S667 | Phosphorylation | Uniprot |
研究背景
Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.
The N-terminus is blocked.
Cytoplasm.
Monomer.
Belongs to the peptidase S9A family.
研究领域
· Organismal Systems > Endocrine system > Renin-angiotensin system. (View pathway)
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