| 产品: | 磷酸化 p62 Dok (Tyr362) 抗体 |
| 货号: | AF3478 |
| 描述: | Rabbit polyclonal antibody to Phospho-p62 Dok (Tyr362) |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 62kDa; 52kD(Calculated). |
| 蛋白号: | Q99704 |
| RRID: | AB_2834916 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3478, RRID:AB_2834916.
展开/折叠
Docking protein 1 62kD; Docking protein 1; DOK 1; DOK1; DOK1_HUMAN; Downstream of tyrosine kinase 1; p62(dok); P62DOK; pp62;
抗原和靶标
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.
- Q99704 DOK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDGAVMEGPLFLQSQRFGTKRWRKTWAVLYPASPHGVARLEFFDHKGSSSGGGRGSSRRLDCKVIRLAECVSVAPVTVETPPEPGATAFRLDTAQRSHLLAADAPSSAAWVQTLCRNAFPKGSWTLAPTDNPPKLSALEMLENSLYSPTWEGSQFWVTVQRTEAAERCGLHGSYVLRVEAERLTLLTVGAQSQILEPLLSWPYTLLRRYGRDKVMFSFEAGRRCPSGPGTFTFQTAQGNDIFQAVETAIHRQKAQGKAGQGHDVLRADSHEGEVAEGKLPSPPGPQELLDSPPALYAEPLDSLRIAPCPSQDSLYSDPLDSTSAQAGEGVQRKKPLYWDLYEHAQQQLLKAKLTDPKEDPIYDEPEGLAPVPPQGLYDLPREPKDAWWCQARVKEEGYELPYNPATDDYAVPPPRSTKPLLAPKPQGPAFPEPGTATGSGIKSHNSALYSQVQKSGASGSWDCGLSRVGTDKTGVKSEGST
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99704 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| Y30 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| R39 | Methylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| T93 | Phosphorylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| T125 | Phosphorylation | Uniprot | |
| Y146 | Phosphorylation | Uniprot | |
| Y203 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| Y296 | Phosphorylation | Uniprot | |
| C308 | S-Nitrosylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| Y315 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| Y337 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| Y362 | Phosphorylation | P00519 (ABL1) , P06213 (INSR) , Q13882 (PTK6) | Uniprot |
| Y377 | Phosphorylation | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| Y398 | Phosphorylation | P07949 (RET) , P06213 (INSR) | Uniprot |
| Y402 | Phosphorylation | Uniprot | |
| T406 | Phosphorylation | Uniprot | |
| Y409 | Phosphorylation | Uniprot | |
| T435 | Phosphorylation | Uniprot | |
| S439 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S443 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S446 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| Y449 | Phosphorylation | Uniprot | |
| S450 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S458 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| T470 | Phosphorylation | Uniprot | |
| T481 | Phosphorylation | Uniprot |
研究背景
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Constitutively tyrosine-phosphorylated. Phosphorylated by TEC (By similarity). Phosphorylated by LYN (By similarity). Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. Phosphorylated on tyrosine residues by SRMS.
Cytoplasm. Nucleus.
Cytoplasm>Perinuclear region.
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.
Interacts with ABL1 (By similarity). Interacts with RasGAP and INPP5D/SHIP1. Interacts directly with phosphorylated ITGB3. Interacts with SRMS (via the SH2 and SH3 domains).
The PTB domain mediates receptor interaction.
Belongs to the DOK family. Type A subfamily.
研究领域
· Human Diseases > Infectious diseases: Viral > Measles.
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