(7)
(8)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13247, RRID:AB_2846266.
展开/折叠
7nAChR; a7 nicotinic acetylcholine receptor; acetylcholine receptor, neuronal nicotinic, alpha-7 subunit; ACHA7_HUMAN; AChR; Acra7; alpha 7 neuronal nicotinic acetylcholine receptor; alpha-7 nicotinic cholinergic receptor subunit; alpha7; cholinergic receptor, neuronal nicotinic, alpha polypeptide 7; cholinergic receptor, nicotinic, alpha 7 (neuronal); cholinergic receptor, nicotinic, alpha 7; cholinergic receptor, nicotinic, alpha polypeptide 7; CHRNA7; CHRNA7-2; NACHRA7; neuronal acetylcholine receptor protein, alpha-7 chain; Neuronal acetylcholine receptor subunit alpha-7;
抗原和靶标
- P36544 ACHA7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRCSPGGVWLALAASLLHVSLQGEFQRKLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNVSEYPGVKTVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDVQHCKLKFGSWSYGGWSLDLQMQEADISGYIPNGEWDLVGIPGKRSERFYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPDGGKMPKWTRVILLNWCAWFLRMKRPGEDKVRPACQHKQRRCSLASVEMSAVAPPPASNGNLLYIGFRGLDGVHCVPTPDSGVVCGRMACSPTHDEHLLHGGQPPEGDPDLAKILEEVRYIANRFRCQDESEAVCSEWKFAACVVDRLCLMAFSVFTIICTIGILMSAPNFVEAVSKDFA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P36544 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y30 | Phosphorylation | Uniprot | |
| T83 | Phosphorylation | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| Y217 | Phosphorylation | Uniprot | |
| T221 | Phosphorylation | Uniprot | |
| T225 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| Y386 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y442 | Phosphorylation | P12931 (SRC) | Uniprot |
研究背景
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Homopentamer (By similarity). Interacts with RIC3; which is required for proper folding and assembly. Interacts with LYPD6. Interacts with the alpha-conotoxin RgIA (By similarity). Interacts with alpha-conotoxins ImI and ImII.
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.
研究领域
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
· Human Diseases > Cancers: Overview > Chemical carcinogenesis.
· Organismal Systems > Nervous system > Cholinergic synapse.
文献引用
Application: WB Species: Rat Sample: RAW264.7 Cells
限制条款
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